中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
题名: The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes
作者: Li, Huatao1, 5; Jiang, Weidan1, 2, 3; Liu, Yang1, 2, 3; Jiang, Jun1, 2, 3; Zhang, Yongan4; Wu, Pei1, 2, 3; Zhao, Juan1, 2, 3; Duan, Xudong1, 2, 3; Zhou, Xiaoqiu1, 2, 3; Feng, Lin1, 2, 3
关键词: Hydroxyl radicals ; Fish erythrocytes ; Apoptosis ; Glutamine ; Metabolites
刊名: FREE RADICAL BIOLOGY AND MEDICINE
发表日期: 2016-03-01
DOI: 10.1016/j.freeradbiomed.2016.01.007
卷: 92, 页:126-140
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Endocrinology & Metabolism
研究领域[WOS]: Biochemistry & Molecular Biology ; Endocrinology & Metabolism
英文摘要: The present study explored the apoptosis pathways in hydroxyl radicals ((OH)-O-center dot)-induced carp erythrocytes. Carp erythrocytes were treated with the caspase inhibitors in physiological carp saline (PCS) or Ca2+-free PCS in the presence of 40 mu M FeSO4/20 mu M H2O2. The results showed that the generation of reactive oxygen species (ROS), the release of cytochrome c and DNA fragmentation were caspase-dependent, and Ca2+ was involved in calpain activation and phosphatidylserine (PS) exposure in (OH)-O-center dot-induced carp erythrocytes. Moreover, the results suggested that caspases were involved in PS exposure, and Ca2+ was involved in DNA fragmentation in (OH)-O-center dot-induced fish erythrocytes. These results demonstrated that there might be two apoptosis pathways in fish erythrocytes, one is the caspase and cytochrome c-dependent apoptosis that is similar to that in mammal nucleated cells, the other is the Ca2+-involved apoptosis that was similar to that in mammal non-nucleated erythrocytes. So, fish erythrocytes may be used as a model for studying oxidative stress and apoptosis in mammal cells. Furthermore, the present study investigated the effects of glutamine (Gln)'s metabolites [alanine (Ala), citrulline (Cit), proline (Pro) and their combination (Ala(10)Pro(4)Cit(1))] on the pathways of apoptosis in fish erythrocytes. The results displayed that Ala, Cit, Pro and Ala(10)Pro(4)Cit(1) effectively suppressed ROS generation, cytochrome c release, activation of caspase-3, caspase-8 and caspase-9 at the physiological concentrations, prevented Ca2+ influx, calpain activation, PS exposure, DNA fragmentation and the degradation of the cytoskeleton and oxidation of membrane and hemoglobin (Hb) and increased activity of anti-hydroxyl radical (AHR) in (OH)-O-center dot-induced carp erythrocytes. Ala(10)Pro(4)Cit(1) produced a synergistic effect of inhibited oxidative stress and apoptosis in fish erythrocytes. These results demonstrated that Ala, Cit, Pro and their combination can protect mammal erythrocytes and nucleated cells against oxidative stress and apoptosis. The studies supported the use of Gln, Ala, Cit and Pro as oxidative stress and apoptosis inhibitors in mammal cells and the hypothesis that the inhibited effects of Gln on oxidative stress and apoptosis are at least partly dependent on that of its metabolites in mammalian. (C) 2016 Elsevier Inc. All rights reserved.
关键词[WOS]: RED-BLOOD-CELLS ; CYTOCHROME-C RELEASE ; N-TERMINAL KINASE ; OXIDATIVE STRESS ; HYDROGEN-PEROXIDE ; LIPID-PEROXIDATION ; AMINO-ACIDS ; CYPRINUS-CARPIO ; SMALL-INTESTINE ; L-ARGININE
语种: 英语
项目资助者: National 973 Project of China(2014CB138600) ; National Science Foundation of China(30771671 ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 30871926) ; 2013NC0045)
WOS记录号: WOS:000370645400011
ISSN号: 0891-5849
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27725
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
1-s2.0-S0891584916000083-main.pdf(2164KB)----开放获取--View Download

作者单位: 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
5.Neijiang Normal Univ, Conservat & Utilizat Fishes Resources Upper Reach, Neijiang 641000, Sichuan, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, Huatao]'s Articles
[Jiang, Weidan]'s Articles
[Liu, Yang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, Huatao]‘s Articles
[Jiang, Weidan]‘s Articles
[Liu, Yang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 1-s2.0-S0891584916000083-main.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace