IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
Expression and localization of grass carp pkc-theta (protein kinase C theta) gene after its activation
Mehjabin, Rumana1,2; Chen, Liangming1,2; Huang, Rong1; Zhu, Denghui1,2; Yang, Cheng1,2; Li, Yongming1; Liao, Lanjie1; He, Libo1; Zhu, Zuoyan1; Wang, Yaping1
Corresponding AuthorHuang, Rong(huangrong@ihb.ac.cn) ; Wang, Yaping(wangyp.ihb@gmail.com)
2019-04-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume87Pages:788-795
AbstractHaemorrhagic disease caused by grass carp reovirus (GCRV) can result in large-scale death of young grass carp, leading to irreparable economic losses that seriously affect large-scale breeding. Protein kinase C (PKC, also known as PRKC) represents a family of serine/threonine protein kinases that includes multiple isozymes in many species. Among these, PKC-theta (PKC theta, also written as PRKCQ) is a novel isoform, mainly expressed in T cells, that is known to be involved in immune system function in mammals. To date, no research on immunological functions of fish Pkc-theta has been reported. To address this issue, we cloned the grass carp pkc-theta gene. Phylogenetic and syntenic analysis showed that this gene is the most evolutionarily conserved relative to zebrafish. Real-time quantitative PCR (RT-qPCR) indicated that pkc-theta was expressed at high levels in the gills and spleen of healthy grass carp. Infection with GCRV down regulated pkc-theta expression in the gills and spleen. Gene products that function upstream and downstream of pkc-theta were up regulated in the gill, but were down-regulated in the spleen. These results suggest that direct or indirect targeting of pkc-theta by GCRV may help the virus evade host immune defences in the spleen. Phorbol ester (PMA) treatment of Jurkat T cells induced translocation of grass carp Pkc-theta from the cytoplasm to the plasma membrane. This response to PMA suggests evolutionary conservation of an immune response function in fish Pkc-theta, as well as conservation of its sequence and structural domains. This study expanded our knowledge of the fish PKC gene family, and explored the role of pkc-theta in function of the grass carp immune system, providing new insights which may facilitate further studies of its biological functions.
KeywordGrass carp Grass carp reovirus (GCRV) Protein kinase C (PKC) Host immune defences
DOI10.1016/j.fsi.2019.01.057
Funding OrganizationStrategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Indexed BySCI ; SCI
Language英语
Funding ProjectStrategic Priority Research Program of the Chinese Academy of Sciences[XDA08030203] ; National Natural Science Foundation of China[31572614] ; National Natural Science Foundation of China[31721005]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000464086600087
WOS KeywordCTENOPHARYNGODON-IDELLUS ; MOLECULAR-CLONING ; DRUG TARGET ; T-CELLS ; BINDING ; FAMILY ; DOMAIN
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Funding OrganizationStrategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27720
Collection鱼类生物学及渔业生物技术研究中心
Corresponding AuthorHuang, Rong; Wang, Yaping
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Mehjabin, Rumana,Chen, Liangming,Huang, Rong,et al. Expression and localization of grass carp pkc-theta (protein kinase C theta) gene after its activation[J]. FISH & SHELLFISH IMMUNOLOGY,2019,87:788-795.
APA Mehjabin, Rumana.,Chen, Liangming.,Huang, Rong.,Zhu, Denghui.,Yang, Cheng.,...&Wang, Yaping.(2019).Expression and localization of grass carp pkc-theta (protein kinase C theta) gene after its activation.FISH & SHELLFISH IMMUNOLOGY,87,788-795.
MLA Mehjabin, Rumana,et al."Expression and localization of grass carp pkc-theta (protein kinase C theta) gene after its activation".FISH & SHELLFISH IMMUNOLOGY 87(2019):788-795.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Mehjabin, Rumana]'s Articles
[Chen, Liangming]'s Articles
[Huang, Rong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Mehjabin, Rumana]'s Articles
[Chen, Liangming]'s Articles
[Huang, Rong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Mehjabin, Rumana]'s Articles
[Chen, Liangming]'s Articles
[Huang, Rong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.