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Establishment and field applications of real-time PCR methods for the quantification of potential MIB-producing cyanobacteria in aquatic systems
Wang, Zhongjie1,2; Song, Gaofei2; Shao, Jihai3; Tan, Wenhua4; Li, Yeguang1; Li, Renhui2
2016-02-01
Source PublicationJOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
Volume28Issue:1Pages:325-333
Abstract2-Methylisoborneol (MIB), along with geosmin, has been frequently associated with taste and odor (T&O) events in waters, and cyanobacterial species were considered the main producers in freshwater ecosystems. The detection and quantification of genes responsible for the synthesis of these odors on-site could be valuable for the prediction and treatment of T&O. In this study, SYBR Green and TaqMan real-time quantitative polymerase chain reaction (qPCR) assays were established on the basis of conserved regions in cyanobacterial MIB cyclase gene (mic) and applied to the quantification of potential MIB-producing cyanobacteria in waters. These two qPCR assays showed the prescribed values of amplification efficiency (0.976 and 1.023, respectively) with linearity (R (2) > 0.99) in the range of 10(1) to 10(7) mic copies. Examination on environmental samples and cyanobacterial cultures indicated that qPCRs were rarely inhibited by background biomass, and the limit of quantification was at the level of 10(3) mic copies L-1 in natural waters. The seasonal variations of mic abundances in Lushui Reservoir, Donghu Lake, and Qinghai Lake of China were successfully quantified using the two qPCR protocols and indicated the applicability of these assays in field monitoring. Regression analysis revealed the positive correlation between mic abundance and MIB concentration, but no significant linear correlation was found between MIB-producing cyanobacteria and total cyanobacteria in investigated waters. The qPCR assays developed in this study could rapidly and reliably detect and quantify the abundance of potential cyanobacterial MIB producers, thereby providing a valuable tool for the prediction and early detection of T&O events.
SubtypeArticle
Keyword2-methylisoborneol Taste And Odor Mib Cyclase Gene Real-time Pcr Cyanobacteria
DOI10.1007/s10811-015-0529-1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNSFC-JST(41261140337) ; NSFC-JST(41261140337) ; National Water Science and Technology Projects(2012ZX07105-004) ; National Water Science and Technology Projects(2012ZX07105-004) ; NSFC-JST(41261140337) ; NSFC-JST(41261140337) ; National Water Science and Technology Projects(2012ZX07105-004) ; National Water Science and Technology Projects(2012ZX07105-004)
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS KeywordQUANTITATIVE PCR ; MOLECULAR-DETECTION ; GREAT-LAKES ; OFF-FLAVORS ; IN-SOURCE ; GEOSMIN ; 2-METHYLISOBORNEOL ; ODOR ; IDENTIFICATION ; BIOSYNTHESIS
WOS IDWOS:000369243300033
Funding OrganizationNSFC-JST(41261140337) ; NSFC-JST(41261140337) ; National Water Science and Technology Projects(2012ZX07105-004) ; National Water Science and Technology Projects(2012ZX07105-004) ; NSFC-JST(41261140337) ; NSFC-JST(41261140337) ; National Water Science and Technology Projects(2012ZX07105-004) ; National Water Science and Technology Projects(2012ZX07105-004)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27713
Collection藻类生物学及应用研究中心
Affiliation1.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan Bot Garden, Wuhan 430074, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
3.Hunan Agr Univ, Resources & Environm Coll, Changsha 410128, Hunan, Peoples R China
4.Max Planck Inst Chem Ecol, Dept Bioorgan Chem, Hans Knoll Str 8, D-07745 Jena, Germany
Recommended Citation
GB/T 7714
Wang, Zhongjie,Song, Gaofei,Shao, Jihai,et al. Establishment and field applications of real-time PCR methods for the quantification of potential MIB-producing cyanobacteria in aquatic systems[J]. JOURNAL OF APPLIED PHYCOLOGY,2016,28(1):325-333.
APA Wang, Zhongjie,Song, Gaofei,Shao, Jihai,Tan, Wenhua,Li, Yeguang,&Li, Renhui.(2016).Establishment and field applications of real-time PCR methods for the quantification of potential MIB-producing cyanobacteria in aquatic systems.JOURNAL OF APPLIED PHYCOLOGY,28(1),325-333.
MLA Wang, Zhongjie,et al."Establishment and field applications of real-time PCR methods for the quantification of potential MIB-producing cyanobacteria in aquatic systems".JOURNAL OF APPLIED PHYCOLOGY 28.1(2016):325-333.
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