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Genome analysis and gene nblA identification of Microcystis aeruginosa myovirus (MaMV-DC) reveal the evidence for horizontal gene transfer events between cyanomyovirus and host
Ou, Tong; Gao, Xiao-Chan; Li, San-Hua; Zhang, Qi-Ya
2015-12-01
Source PublicationJOURNAL OF GENERAL VIROLOGY
ISSN0022-1317
Volume96Pages:3681-3697
AbstractThe genome sequence, genetic characterization and nblA gene function of Microcystis aeruginosa myovirus isolated from Lake Dianchi in China (MaMV-DC) have been analysed. The genome DNA is 169 223 bp long, with 170 predicted protein-coding genes (001L-170L) and a tRNA gene. About one-sixth of these genes have homologues in the host cyanobacteria M. aeruginosa. The genome carries a gene homologous to host nblA, which encodes a protein involved in the degradation of cyanobacterial phycobilisome. Its expression during MaMV-DC infection was confirmed by reverse transcriptase PCR and Western blot detection and abundant expression was companied by the significant decline of phycocyanin content and massive release of progeny MaMV-DC. In addition, expressing MaMV-DC nblA reduced the phycocyanin peak and the phycocyanin to chlorophyll ratio in model cyanobacteria. These results confirm that horizontal gene transfer events have occurred between cyanobacterial host and cyanomyovirus and suggest that MaMV-DC carrying host-derived genes (such as 005L, that codes for NblA) is responsible for more efficient expression of cyanophage genes and release of progeny cyanophage. This study provides novel insight into the horizontal gene transfer in cyanophage and the interactions between cyanophage and their host.
SubtypeArticle
DOI10.1099/jgv.0.000290
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31270213 ; National Natural Science Foundation of China(31270213 ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ12) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ12) ; 31430091) ; 31430091) ; National Natural Science Foundation of China(31270213 ; National Natural Science Foundation of China(31270213 ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ12) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ12) ; 31430091) ; 31430091)
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Virology
WOS SubjectBiotechnology & Applied Microbiology ; Virology
WOS KeywordMARINE SYNECHOCOCCUS STRAINS ; PHYCOBILISOME DEGRADATION ; PHOTOSYNTHESIS GENES ; ACQUIRED-RESISTANCE ; CYANOPHAGE ; SEQUENCE ; PHAGES ; PROCHLOROCOCCUS ; DIVERSITY ; VIRUSES
WOS IDWOS:000369521800024
Funding OrganizationNational Natural Science Foundation of China(31270213 ; National Natural Science Foundation of China(31270213 ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ12) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ12) ; 31430091) ; 31430091) ; National Natural Science Foundation of China(31270213 ; National Natural Science Foundation of China(31270213 ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ12) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ12) ; 31430091) ; 31430091)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27707
Collection鱼类生物学及渔业生物技术研究中心
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Ou, Tong,Gao, Xiao-Chan,Li, San-Hua,et al. Genome analysis and gene nblA identification of Microcystis aeruginosa myovirus (MaMV-DC) reveal the evidence for horizontal gene transfer events between cyanomyovirus and host[J]. JOURNAL OF GENERAL VIROLOGY,2015,96:3681-3697.
APA Ou, Tong,Gao, Xiao-Chan,Li, San-Hua,&Zhang, Qi-Ya.(2015).Genome analysis and gene nblA identification of Microcystis aeruginosa myovirus (MaMV-DC) reveal the evidence for horizontal gene transfer events between cyanomyovirus and host.JOURNAL OF GENERAL VIROLOGY,96,3681-3697.
MLA Ou, Tong,et al."Genome analysis and gene nblA identification of Microcystis aeruginosa myovirus (MaMV-DC) reveal the evidence for horizontal gene transfer events between cyanomyovirus and host".JOURNAL OF GENERAL VIROLOGY 96(2015):3681-3697.
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