IHB OpenIR  > 藻类生物学及应用研究中心
The Cytochrome b(6)f Complex Is Not Involved in Cyanobacterial State Transitions
Calzadilla, Pablo, I1; Zhan, Jiao1,2; Setif, Pierre1; Lemaire, Claire1; Solymosi, Daniel3; Battchikova, Natalia3; Wang, Qiang2,4; Kirilovsky, Diana1
Corresponding AuthorKirilovsky, Diana(diana.kirilovsky@cea.fr)
2019-04-01
Source PublicationPLANT CELL
ISSN1040-4651
Volume31Issue:4Pages:911-931
AbstractPhotosynthetic organisms must sense and respond to fluctuating environmental conditions in order to perform efficient photosynthesis and to avoid the formation of dangerous reactive oxygen species. The excitation energy arriving at each photosystem permanently changes due to variations in the intensity and spectral properties of the absorbed light. Cyanobacteria, like plants and algae, have developed a mechanism, named "state transitions," that balances photosystem activities. Here, we characterize the role of the cytochrome b(6)f complex and phosphorylation reactions in cyanobacterial state transitions using Synechococcus elongatus PCC 7942 and Synechocystis PCC 6803 as model organisms. First, large photosystem II (PSII) fluorescence quenching was observed in State II, a result that does not appear to be related to energy transfer from PSII to PSI (spillover). This membrane-associated process was inhibited by betaine, Suc, and high concentrations of phosphate. Then, using different chemicals affecting the plastoquinone pool redox state and cytochrome b(6)f activity, we demonstrate that this complex is not involved in state transitions in S. elongatus or Synechocystis PCC6803. Finally, by constructing and characterizing 21 protein kinase and phosphatase mutants and using chemical inhibitors, we demonstrate that phosphorylation reactions are not essential for cyanobacterial state transitions. Thus, signal transduction is completely different in cyanobacterial and plant (green alga) state transitions.
DOI10.1105/tpc.18.00916
Funding OrganizationAgence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; European Union ; European Union ; European Union ; European Union ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; European Union ; European Union ; European Union ; European Union ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; European Union ; European Union ; European Union ; European Union ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; European Union ; European Union ; European Union ; European Union ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI)
Indexed BySCI ; SCI
Language英语
Funding ProjectAgence Nationale de la Recherche's RECYFUEL project[ANR-16-CE05-0026] ; European Union[675006] ; Centre National de la Recherche Scientifique (CNRS) ; Commissariat a l'Energie Atomique (CEA) ; National Natural Science Foundation of China[31700107] ; National Natural Science Foundation of China[31770128] ; French Infrastructure for Integrated Structural Biology (FRISBI)[ANR-10-INBS-05]
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS IDWOS:000466314500012
WOS KeywordCHLOROPHYLL-A FLUORESCENCE ; PHOTOSYSTEM-II FLUORESCENCE ; EXCITATION-ENERGY TRANSFER ; GLNB GENE-PRODUCT ; SP PCC 6803 ; PROTEIN-KINASE ; CHLAMYDOMONAS-REINHARDTII ; REDOX STATE ; MODEL CYANOBACTERIUM ; THYLAKOID MEMBRANES
PublisherAMER SOC PLANT BIOLOGISTS
Funding OrganizationAgence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; European Union ; European Union ; European Union ; European Union ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; European Union ; European Union ; European Union ; European Union ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; European Union ; European Union ; European Union ; European Union ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; Agence Nationale de la Recherche's RECYFUEL project ; European Union ; European Union ; European Union ; European Union ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Centre National de la Recherche Scientifique (CNRS) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; Commissariat a l'Energie Atomique (CEA) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI) ; French Infrastructure for Integrated Structural Biology (FRISBI)
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27642
Collection藻类生物学及应用研究中心
Corresponding AuthorKirilovsky, Diana
Affiliation1.Univ Paris Saclay, Univ Paris Sud, CNRS, I2BC,Commissariat Energie Atom & Energies Alterna, F-91198 Gif Sur Yvette, France
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
3.Univ Turku, Fac Sci & Engn, Biochem Dept, Mol Plant Biol Lab, FI-20014 Turku, Finland
4.Henan Univ, Sch Life Sci, Key Lab Plant Stress Biol, State Key Lab Cotton Biol, Kaifeng 475004, Peoples R China
Recommended Citation
GB/T 7714
Calzadilla, Pablo, I,Zhan, Jiao,Setif, Pierre,et al. The Cytochrome b(6)f Complex Is Not Involved in Cyanobacterial State Transitions[J]. PLANT CELL,2019,31(4):911-931.
APA Calzadilla, Pablo, I.,Zhan, Jiao.,Setif, Pierre.,Lemaire, Claire.,Solymosi, Daniel.,...&Kirilovsky, Diana.(2019).The Cytochrome b(6)f Complex Is Not Involved in Cyanobacterial State Transitions.PLANT CELL,31(4),911-931.
MLA Calzadilla, Pablo, I,et al."The Cytochrome b(6)f Complex Is Not Involved in Cyanobacterial State Transitions".PLANT CELL 31.4(2019):911-931.
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