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Solid Matrix-Supported Supercritical CO2 Enhances Extraction of -Linolenic Acid from the Cyanobacterium Arthrospira (Spirulina) platensis and Bioactivity Evaluation of the Molecule in Zebrafish
Yang, Xiaohong1,5; Li, Yi2,6; Li, Yanhua1; Ye, Ding2,6; Yuan, Li1; Sun, Yonghua2,6; Han, Danxiang1,4; Hu, Qiang1,2,3,4,7
Corresponding AuthorHan, Danxiang(danxianghan@ihb.ac.cn) ; Hu, Qiang(huqiang@ihb.ac.cn)
2019-04-01
Source PublicationMARINE DRUGS
ISSN1660-3397
Volume17Issue:4Pages:20
AbstractMarine cyanobacteria represent a large untapped source of functional glycolipids enriched with polyunsaturated fatty acids (PUFAs) for human health. However, advanced methods for scalable isolation of diverse species containing high-purity PUFA-rich glycolipids will have to be developed and their possible pharmaceutical and nutraceutical functions identified. This paper introduces a novel solid matrix-supported supercritical CO2 extraction method for scalable isolation of the PUFA -linolenic acid (GLA)-enriched glycolipids from the cyanobacterium Arthrospira (Spirulina) platensis, which has been the most widely used among microalgae in the nutraceutical and pharmaceutical industries. Of various porous materials studied, diatomite was the best to facilitate extraction of GLA-rich glycolipids, resulting in an extraction efficiency of 98%. Gamma-linolenic acid made up 35% of total fatty acids (TFAs) in the extracts, which was considerably greater than that obtained with ethanol (26%), Bligh and Dyer (24%), and in situ transesterification (24%) methods, respectively. Lipidomics analysis revealed that GLA was exclusively associated with galactolipids. Pharmaceutical functions of GLA-rich galactolipids were investigated on a zebrafish caudal fin regeneration model. The results suggested that GLA extracted from A. platensis possessed anti-oxidative, anti-inflammatory, and anti-allergic activities, which acted in a concerted manner to promote post-injury regeneration of zebrafish.
KeywordArthrospira (Spirulina) platensis porous materials diatomite solid matrix-supported supercritical CO2 (SMSSC-CO2) -linolenic acid (GLA) bioactivity zebrafish
DOI10.3390/md17040203
Funding OrganizationState Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass
Indexed BySCI ; SCI
Language英语
Funding ProjectState Development & Investment Corporation (SDIC) ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences[2016FBZ06] ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences[2016FBZ03] ; One Hundred Talents Program of Chinese Academy of Sciences[Y623031Z01] ; Beijing Key Laboratory of Algae Biomass[SDICMBC-00003]
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal
WOS IDWOS:000467307100010
WOS KeywordCARBON-DIOXIDE EXTRACTION ; ELECTROSPRAY IONIZATION ; RHEUMATOID-ARTHRITIS ; FUNCTIONAL COMPOUNDS ; MASS-SPECTROMETRY ; FLUID EXTRACTION ; OIL ; BIOMASS ; SUPPLEMENTATION ; PURIFICATION
PublisherMDPI
Funding OrganizationState Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Development & Investment Corporation (SDIC) ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; One Hundred Talents Program of Chinese Academy of Sciences ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass ; Beijing Key Laboratory of Algae Biomass
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27607
Collection藻类生物技术和生物能源研发中心
Corresponding AuthorHan, Danxiang; Hu, Qiang
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
5.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
6.Chinese Acad Sci, Univ Chinese Acad Sci, Innovat Acad Seed Design, Coll Adv Agr Sci, Beijing 100049, Peoples R China
7.State Dev & Investment Corp, SDIC Biotech Investment Co LTD, Microalgae Biotechnol Ctr, Beijing Key Lab Algae Biomass, Beijing 100142, Peoples R China
Recommended Citation
GB/T 7714
Yang, Xiaohong,Li, Yi,Li, Yanhua,et al. Solid Matrix-Supported Supercritical CO2 Enhances Extraction of -Linolenic Acid from the Cyanobacterium Arthrospira (Spirulina) platensis and Bioactivity Evaluation of the Molecule in Zebrafish[J]. MARINE DRUGS,2019,17(4):20.
APA Yang, Xiaohong.,Li, Yi.,Li, Yanhua.,Ye, Ding.,Yuan, Li.,...&Hu, Qiang.(2019).Solid Matrix-Supported Supercritical CO2 Enhances Extraction of -Linolenic Acid from the Cyanobacterium Arthrospira (Spirulina) platensis and Bioactivity Evaluation of the Molecule in Zebrafish.MARINE DRUGS,17(4),20.
MLA Yang, Xiaohong,et al."Solid Matrix-Supported Supercritical CO2 Enhances Extraction of -Linolenic Acid from the Cyanobacterium Arthrospira (Spirulina) platensis and Bioactivity Evaluation of the Molecule in Zebrafish".MARINE DRUGS 17.4(2019):20.
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