| Functional Characterization of Dark Sleeper (Odontobutis obscura) TBK1 on IFN Regulation | |
| Chen, Jian1; Li, Zhuo Cong2,3; Lu, Long Feng2,3; Li, Pei1; Li, Xi-Yin2,3; Li, Shun2,3,4 | |
| Corresponding Author | Li, Shun(bob@ihb.ac.cn) |
| 2019-05-03 | |
| Source Publication | FRONTIERS IN IMMUNOLOGY
 |
| ISSN | 1664-3224 |
| Volume | 10Pages:11 |
| Abstract | In East Asia, the dark sleeper, Odontobutis obscura (O. obscura) is a crucial commercial species of freshwater fish; however, its molecular biology research is still undeveloped, including its innate immune system, which is pivotal to antiviral responses. In this study, we cloned and identified the characterization and kinase function of dark sleeper TANK-binding kinase 1 (TBK1), supplementing the evidence of the conservation of this classical factor in fish. First, the ORF of Odontobutis obscurus (O. obscura) TBK1 (OdTBK1) was cloned from liver tissue by RACE-PCR. Subsequent nucleic acid and amino acid sequence analysis suggested that OdTBK1 is homologous with other fish TBK1, and the N-terminal Serine/Threonine protein kinases catalytic domain (S_TKc) and C-terminal coiled coil domain (CCD) are conserved. Subsequently, the cellular distribution demonstrated that OdTBK1 was located in the cytoplasm region. With regard to the identification of functions, OdTBK1 activated several interferon (IFN) promoters' activity and induced downstream IFN-stimulated genes (ISGs) expression. In a canonical manner, wild-type OdTBK1 significantly phosphorylated interferon regulatory factor 3 (IRF3) but failed when the N-terminal region was truncated. Furthermore, overexpression of OdTBK1 decreased viral proliferation remarkably. Collectively, these data systematically analyzed the characterization and function of OdTBK1, initiating the study of the innate antiviral response of dark sleeper. |
| Keyword | TBK1 IFN RLRs antiviral Odontobutis obscura |
| DOI | 10.3389/fimmu.2019.00985 |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31502200] |
| WOS Research Area | Immunology |
| WOS Subject | Immunology |
| WOS ID | WOS:000467335700002 |
| WOS Keyword | INNATE IMMUNE-RESPONSE ; TANK-BINDING KINASE-1 ; KAPPA-B ACTIVATION ; IFN-PHI-1 PRODUCTION ; RIG-I ; FISH ; PROTEIN ; PLAYS ; GENE ; IRF3 |
| Publisher | FRONTIERS MEDIA SA |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/27595 |
| Collection | 水生生物分子与细胞生物学研究中心 |
| Corresponding Author | Li, Shun |
| Affiliation | 1.Wuhan Acad Agr Sci, Fisheries Res Inst, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China 3.Univ Chinese Acad Sci, Wuhan, Hubei, Peoples R China 4.Minist Agr, Key Lab Aquaculture Dis Control, Wuhan, Hubei, Peoples R China |
| Recommended Citation GB/T 7714 | Chen, Jian,Li, Zhuo Cong,Lu, Long Feng,et al. Functional Characterization of Dark Sleeper (Odontobutis obscura) TBK1 on IFN Regulation[J]. FRONTIERS IN IMMUNOLOGY,2019,10:11. |
| APA | Chen, Jian,Li, Zhuo Cong,Lu, Long Feng,Li, Pei,Li, Xi-Yin,&Li, Shun.(2019).Functional Characterization of Dark Sleeper (Odontobutis obscura) TBK1 on IFN Regulation.FRONTIERS IN IMMUNOLOGY,10,11. |
| MLA | Chen, Jian,et al."Functional Characterization of Dark Sleeper (Odontobutis obscura) TBK1 on IFN Regulation".FRONTIERS IN IMMUNOLOGY 10(2019):11. |
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