IHB OpenIR  > 水生生物分子与细胞生物学研究中心
Functional Characterization of Dark Sleeper (Odontobutis obscura) TBK1 on IFN Regulation
Chen, Jian1; Li, Zhuo Cong2,3; Lu, Long Feng2,3; Li, Pei1; Li, Xi-Yin2,3; Li, Shun2,3,4
Corresponding AuthorLi, Shun(bob@ihb.ac.cn)
2019-05-03
Source PublicationFRONTIERS IN IMMUNOLOGY
ISSN1664-3224
Volume10Pages:11
AbstractIn East Asia, the dark sleeper, Odontobutis obscura (O. obscura) is a crucial commercial species of freshwater fish; however, its molecular biology research is still undeveloped, including its innate immune system, which is pivotal to antiviral responses. In this study, we cloned and identified the characterization and kinase function of dark sleeper TANK-binding kinase 1 (TBK1), supplementing the evidence of the conservation of this classical factor in fish. First, the ORF of Odontobutis obscurus (O. obscura) TBK1 (OdTBK1) was cloned from liver tissue by RACE-PCR. Subsequent nucleic acid and amino acid sequence analysis suggested that OdTBK1 is homologous with other fish TBK1, and the N-terminal Serine/Threonine protein kinases catalytic domain (S_TKc) and C-terminal coiled coil domain (CCD) are conserved. Subsequently, the cellular distribution demonstrated that OdTBK1 was located in the cytoplasm region. With regard to the identification of functions, OdTBK1 activated several interferon (IFN) promoters' activity and induced downstream IFN-stimulated genes (ISGs) expression. In a canonical manner, wild-type OdTBK1 significantly phosphorylated interferon regulatory factor 3 (IRF3) but failed when the N-terminal region was truncated. Furthermore, overexpression of OdTBK1 decreased viral proliferation remarkably. Collectively, these data systematically analyzed the characterization and function of OdTBK1, initiating the study of the innate antiviral response of dark sleeper.
KeywordTBK1 IFN RLRs antiviral Odontobutis obscura
DOI10.3389/fimmu.2019.00985
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31502200]
WOS Research AreaImmunology
WOS SubjectImmunology
WOS IDWOS:000467335700002
WOS KeywordINNATE IMMUNE-RESPONSE ; TANK-BINDING KINASE-1 ; KAPPA-B ACTIVATION ; IFN-PHI-1 PRODUCTION ; RIG-I ; FISH ; PROTEIN ; PLAYS ; GENE ; IRF3
PublisherFRONTIERS MEDIA SA
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27595
Collection水生生物分子与细胞生物学研究中心
Corresponding AuthorLi, Shun
Affiliation1.Wuhan Acad Agr Sci, Fisheries Res Inst, Wuhan, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Wuhan, Hubei, Peoples R China
4.Minist Agr, Key Lab Aquaculture Dis Control, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Chen, Jian,Li, Zhuo Cong,Lu, Long Feng,et al. Functional Characterization of Dark Sleeper (Odontobutis obscura) TBK1 on IFN Regulation[J]. FRONTIERS IN IMMUNOLOGY,2019,10:11.
APA Chen, Jian,Li, Zhuo Cong,Lu, Long Feng,Li, Pei,Li, Xi-Yin,&Li, Shun.(2019).Functional Characterization of Dark Sleeper (Odontobutis obscura) TBK1 on IFN Regulation.FRONTIERS IN IMMUNOLOGY,10,11.
MLA Chen, Jian,et al."Functional Characterization of Dark Sleeper (Odontobutis obscura) TBK1 on IFN Regulation".FRONTIERS IN IMMUNOLOGY 10(2019):11.
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