| Transcriptomic profiling of chemical exposure reveals roles of Yap1 in protecting yeast cells from oxidative and other types of stresses | |
| Zhang, Chao1,2; Li, Zhouquan1,2; Zhang, Xiaohua1; Yuan, Li1; Dai, Heping1; Xiao, Wei3,4 | |
| 2016 | |
| Source Publication | YEAST
 |
| ISSN | 0749-503X |
| Volume | 33Issue:1Pages:5-19 |
| Abstract | Transcriptomic profiles are generated by comparing wild-type and the yeast yap1 mutant to various chemicals in an attempt to establish a correlation between this gene mutation and chemical exposure. Test chemicals include ClonNAT as a non-genotoxic agent, methyl methanesulphonate (MMS) as an alkylating agent, tert-butyl hydroperoxide (t-BHP) as an oxidative agent and the mixture of t-BHP and MMS to reflect complex natural exposure. Differentially expressed genes (DEGs) were identified and specific DEGs were obtained by excluding overlapping DEGs with the control group. In the MMS exposure group, deoxyribonucleotide biosynthetic processes were upregulated, while oxidation-reduction processes were downregulated. In the t-BHP exposure group, metabolic processes were upregulated while peroxisome and ion transport pathways were downregulated. In the mixture exposure group, the proteasome pathway was upregulated, while the aerobic respiration was downregulated. Homologue analysis of DEGs related to human diseases showed that many of DEGs were linked to cancer, ageing and neuronal degeneration. These observations confirm that the yap1 mutant is more sensitive to chemicals than wild-type cells and that the susceptible individuals carrying the YAP1-like gene defect may enhance risk to chemical exposure. Hence, this study offers a novel approach to environmental risk assessment, based on the genetic backgrounds of susceptible individuals. Copyright (c) 2015 John Wiley & Sons, Ltd. |
| Subtype | Article |
| Keyword | Saccharomyces Cerevisiae Rna-seq Chemical Exposure Risk Assessment Yap1 |
| DOI | 10.1002/yea.3135 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Funding Organization | Natural Science Foundation of China(21037004) ; Natural Science Foundation of China(21037004) ; State Key Laboratory of Fresh Water Ecology and Biotechnology(2011FBZ10) ; State Key Laboratory of Fresh Water Ecology and Biotechnology(2011FBZ10) ; Natural Science Foundation of China(21037004) ; Natural Science Foundation of China(21037004) ; State Key Laboratory of Fresh Water Ecology and Biotechnology(2011FBZ10) ; State Key Laboratory of Fresh Water Ecology and Biotechnology(2011FBZ10) |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology |
| WOS Subject | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology |
| WOS Keyword | GENOTOXICITY TESTING SYSTEM ; DNA-DAMAGING AGENTS ; RNA-SEQ DATA ; SACCHAROMYCES-CEREVISIAE ; GENE-EXPRESSION ; ENHANCES SENSITIVITY ; ENVIRONMENTAL-HEALTH ; TOXICOGENOMICS ; TOXICOLOGY ; NETWORKS |
| WOS ID | WOS:000367950400002 |
| Funding Organization | Natural Science Foundation of China(21037004) ; Natural Science Foundation of China(21037004) ; State Key Laboratory of Fresh Water Ecology and Biotechnology(2011FBZ10) ; State Key Laboratory of Fresh Water Ecology and Biotechnology(2011FBZ10) ; Natural Science Foundation of China(21037004) ; Natural Science Foundation of China(21037004) ; State Key Laboratory of Fresh Water Ecology and Biotechnology(2011FBZ10) ; State Key Laboratory of Fresh Water Ecology and Biotechnology(2011FBZ10) |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/27592 |
| Collection | 藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心 |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Capital Normal Univ, Coll Life Sci, Beijing, Peoples R China 4.Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK, Canada |
| Recommended Citation GB/T 7714 | Zhang, Chao,Li, Zhouquan,Zhang, Xiaohua,et al. Transcriptomic profiling of chemical exposure reveals roles of Yap1 in protecting yeast cells from oxidative and other types of stresses[J]. YEAST,2016,33(1):5-19. |
| APA | Zhang, Chao,Li, Zhouquan,Zhang, Xiaohua,Yuan, Li,Dai, Heping,&Xiao, Wei.(2016).Transcriptomic profiling of chemical exposure reveals roles of Yap1 in protecting yeast cells from oxidative and other types of stresses.YEAST,33(1),5-19. |
| MLA | Zhang, Chao,et al."Transcriptomic profiling of chemical exposure reveals roles of Yap1 in protecting yeast cells from oxidative and other types of stresses".YEAST 33.1(2016):5-19. |
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