Transcriptomic profiling of chemical exposure reveals roles of Yap1 in protecting yeast cells from oxidative and other types of stresses
Zhang, Chao1,2; Li, Zhouquan1,2; Zhang, Xiaohua1; Yuan, Li1; Dai, Heping1; Xiao, Wei3,4
2016
Source PublicationYEAST
ISSN0749-503X
Volume33Issue:1Pages:5-19
AbstractTranscriptomic profiles are generated by comparing wild-type and the yeast yap1 mutant to various chemicals in an attempt to establish a correlation between this gene mutation and chemical exposure. Test chemicals include ClonNAT as a non-genotoxic agent, methyl methanesulphonate (MMS) as an alkylating agent, tert-butyl hydroperoxide (t-BHP) as an oxidative agent and the mixture of t-BHP and MMS to reflect complex natural exposure. Differentially expressed genes (DEGs) were identified and specific DEGs were obtained by excluding overlapping DEGs with the control group. In the MMS exposure group, deoxyribonucleotide biosynthetic processes were upregulated, while oxidation-reduction processes were downregulated. In the t-BHP exposure group, metabolic processes were upregulated while peroxisome and ion transport pathways were downregulated. In the mixture exposure group, the proteasome pathway was upregulated, while the aerobic respiration was downregulated. Homologue analysis of DEGs related to human diseases showed that many of DEGs were linked to cancer, ageing and neuronal degeneration. These observations confirm that the yap1 mutant is more sensitive to chemicals than wild-type cells and that the susceptible individuals carrying the YAP1-like gene defect may enhance risk to chemical exposure. Hence, this study offers a novel approach to environmental risk assessment, based on the genetic backgrounds of susceptible individuals. Copyright (c) 2015 John Wiley & Sons, Ltd.
SubtypeArticle
KeywordSaccharomyces Cerevisiae Rna-seq Chemical Exposure Risk Assessment Yap1
DOI10.1002/yea.3135
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNatural Science Foundation of China(21037004) ; Natural Science Foundation of China(21037004) ; State Key Laboratory of Fresh Water Ecology and Biotechnology(2011FBZ10) ; State Key Laboratory of Fresh Water Ecology and Biotechnology(2011FBZ10) ; Natural Science Foundation of China(21037004) ; Natural Science Foundation of China(21037004) ; State Key Laboratory of Fresh Water Ecology and Biotechnology(2011FBZ10) ; State Key Laboratory of Fresh Water Ecology and Biotechnology(2011FBZ10)
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology
WOS KeywordGENOTOXICITY TESTING SYSTEM ; DNA-DAMAGING AGENTS ; RNA-SEQ DATA ; SACCHAROMYCES-CEREVISIAE ; GENE-EXPRESSION ; ENHANCES SENSITIVITY ; ENVIRONMENTAL-HEALTH ; TOXICOGENOMICS ; TOXICOLOGY ; NETWORKS
WOS IDWOS:000367950400002
Funding OrganizationNatural Science Foundation of China(21037004) ; Natural Science Foundation of China(21037004) ; State Key Laboratory of Fresh Water Ecology and Biotechnology(2011FBZ10) ; State Key Laboratory of Fresh Water Ecology and Biotechnology(2011FBZ10) ; Natural Science Foundation of China(21037004) ; Natural Science Foundation of China(21037004) ; State Key Laboratory of Fresh Water Ecology and Biotechnology(2011FBZ10) ; State Key Laboratory of Fresh Water Ecology and Biotechnology(2011FBZ10)
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Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27592
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Capital Normal Univ, Coll Life Sci, Beijing, Peoples R China
4.Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK, Canada
Recommended Citation
GB/T 7714
Zhang, Chao,Li, Zhouquan,Zhang, Xiaohua,et al. Transcriptomic profiling of chemical exposure reveals roles of Yap1 in protecting yeast cells from oxidative and other types of stresses[J]. YEAST,2016,33(1):5-19.
APA Zhang, Chao,Li, Zhouquan,Zhang, Xiaohua,Yuan, Li,Dai, Heping,&Xiao, Wei.(2016).Transcriptomic profiling of chemical exposure reveals roles of Yap1 in protecting yeast cells from oxidative and other types of stresses.YEAST,33(1),5-19.
MLA Zhang, Chao,et al."Transcriptomic profiling of chemical exposure reveals roles of Yap1 in protecting yeast cells from oxidative and other types of stresses".YEAST 33.1(2016):5-19.
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