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题名: YcgC represents a new protein deacetylase family in prokaryotes
作者: Tu, Shun1, 2; Guo, Shu-Juan1, 2; Chen, Chien-Sheng3; Liu, Cheng-Xi1, 2; Jiang, He-Wei1, 2; Ge, Feng4; Deng, Jiao-Yu5; Zhou, Yi-Ming6; Czajkowsky, Daniel M.7; Li, Yang1, 2; Qi, Bang-Ruo1, 2; Ahn, Young-Hoon8; Cole, Philip A.8; Zhu, Heng8, 9; Tao, Sheng-Ce1, 2, 7
刊名: ELIFE
发表日期: 2015-12-30
DOI: 10.7554/eLife.05322
卷: 4
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biology
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
英文摘要: Reversible lysine acetylation is one of the most important protein posttranslational modifications that plays essential roles in both prokaryotes and eukaryotes. However, only a few lysine deacetylases (KDACs) have been identified in prokaryotes, perhaps in part due to their limited sequence homology. Herein, we developed a 'clip-chip' strategy to enable unbiased, activity-based discovery of novel KDACs in the Escherichia coli proteome. In-depth biochemical characterization confirmed that YcgC is a serine hydrolase involving Ser200 as the catalytic nucleophile for lysine deacetylation and does not use NAD(+) or Zn2+ like other established KDACs. Further, in vivo characterization demonstrated that YcgC regulates transcription by catalyzing deacetylation of Lys52 and Lys62 of a transcriptional repressor RutR. Importantly, YcgC targets a distinct set of substrates from the only known E. coli KDAC CobB. Analysis of YcgC's bacterial homologs confirmed that they also exhibit KDAC activity. YcgC thus represents a novel family of prokaryotic KDACs.
关键词[WOS]: ESCHERICHIA-COLI ; LYSINE ACETYLATION ; SACCHAROMYCES-CEREVISIAE ; SIRT1 DEACETYLASE ; CELL-SURVIVAL ; TRANSCRIPTION ; ACETYLTRANSFERASE ; RESTRICTION ; REGULATOR ; MECHANISM
语种: 英语
项目资助者: National Natural Science Foundation of China(31370750 ; National Institutes of Health(GM62437 ; Flight Attendant Medical Research Institute ; Ministry of Science and Technology of the People's Republic of China(2010CB529205 ; Ministry of Health of the People's Republic of China(2013ZX10003006) ; 31370813 ; RR020839 ; 2012AA020103 ; 31000388) ; GM076102 ; 2012AA020203) ; HG006434)
WOS记录号: WOS:000383873100001
ISSN号: 2050-084X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27581
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Key Lab Syst Biomed, Shanghai, Peoples R China
2.State Key Lab Oncogenes & Related Genes, Shanghai, Peoples R China
3.Natl Cent Univ, Grad Inst Syst Biol & Bioinformat, Jhongli, Taiwan
4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China
5.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Peoples R China
6.Natl Engn Res Ctr Beijing Biochip Technol, Beijing, Peoples R China
7.Shanghai Jiao Tong Univ, Sch Biomed Engn, BioID Ctr, Shanghai, Peoples R China
8.Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 USA
9.Johns Hopkins Univ, Sch Med, High Throughput Biol Ctr, Baltimore, MD USA
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