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Expression of organophosphorus-degradation gene (opd) in aggregating and non-aggregating filamentous nitrogen-fixing cyanobacteria
Li Qiong1,2; Tang Qing1,2; Xu Xudong1; Gao Hong1
2010-11-01
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
Volume28Issue:6Pages:1248-1253
AbstractGenetic engineering in filamentous N(2)-fixing cyanobacteria usually Involves Anabaena sp PCC 7120 and several other non-aggregating species Mass culture and harvest of such species are more energy consuming relative to aggregating species To establish a gene transfer system for aggregating species, we tested many species of Anabaena and Nostoc, and identified Nostoc muscorum FACHB244 as a species that can be genetically manipulated using the conjugative gene transfer system To promote biodegradation of organophosphorus pollutants in aquatic environments, we introduced a plasmid containing the organophosphorus degradation gene (opd) into Anabaena sp PCC 7120 and Nostoc muscorum FACHB244 by conjugation The opd gene was driven by a strong promoter, P(psbA) From both species, we obtained transgenic strains having organophosphorus-degradation activities At 25 degrees C, the whole-cell activities of the transgenic Anabaena and Nostoc strains were 0 163 +/- 0 001 and 0 289 +/- 0 042 unit/mu g Chl a, respectively However, most colonies resulting from the gene transfer showed no activity PCR and DNA sequencing revealed deletions or rearrangements in the plasmid in some of the colonies Expression of the green fluorescent protein gene from the same promoter in Anabaena sp PCC 7120 showed similar results These results suggest that there is the potential to promote the degradation of organophosphorus pollutants with transgenic cyanobacteria and that selection of high-expression transgenic colonies is Important for genetic engineering of Anabaena and Nostoc species For the first time, we established a gene transfer and expression system in an aggregating filamentous N(2)-fixing cyanobacterium The genetic manipulation system of Nostoc muscorum FACHB244 could be utilized in the elimination of pollutants and large-scale production of valuable proteins or metabolites
SubtypeArticle
KeywordCyanobacteria Aggregating Species Conjugative Gene Transfer Organophosphorus-degradation
DOI10.1007/s00343-010-9999-9
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS KeywordANABAENA SP PCC-7120 ; STRAIN PCC 7120 ; PSEUDOMONAS-DIMINUTA ; LARVICIDAL ACTIVITY ; FLAVOBACTERIUM SP ; ISRAELENSIS ; CLONING
WOS IDWOS:000285279700019
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27501
Collection藻类生物学及应用研究中心
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Li Qiong,Tang Qing,Xu Xudong,et al. Expression of organophosphorus-degradation gene (opd) in aggregating and non-aggregating filamentous nitrogen-fixing cyanobacteria[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2010,28(6):1248-1253.
APA Li Qiong,Tang Qing,Xu Xudong,&Gao Hong.(2010).Expression of organophosphorus-degradation gene (opd) in aggregating and non-aggregating filamentous nitrogen-fixing cyanobacteria.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,28(6),1248-1253.
MLA Li Qiong,et al."Expression of organophosphorus-degradation gene (opd) in aggregating and non-aggregating filamentous nitrogen-fixing cyanobacteria".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 28.6(2010):1248-1253.
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