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Isolation and characterization of 15 microsatellite loci in an endemic Chinese cyprinid fish, Pseudogyrincheilus prochilus, and their cross-species amplification in two related species
Shi, Fang1,2,3,4; Xu, Nian1,2; Xiong, Meihua1,2; Wang, Xiang1,2; Yang, Zhong1,2; Que, Yanfu1,2; Zhu, Bin1,2; Hu, Juxiang1,2; Chang, Jianbo1,2
2009-12-01
Source PublicationCONSERVATION GENETICS RESOURCES
ISSN1877-7252
Volume1Issue:1Pages:397-399
AbstractFifteen polymorphic microsatellite loci were developed for an endemic Chinese cyprinid fish, Pseudogyrincheilus prochilus. The number of observed alleles per locus ranged from 2 to 17. The observed and expected heterozygosities ranged from 0.067 to 1.000 and from 0.066 to 0.932, respectively. No significant linkage disequilibrium was detected among the loci. Two loci showed significant deviation from Hardy-Weinberg equilibrium, and one of them had evidence for null alleles. Fourteen primer pairs cross-amplified in Garra pingi pingi, and 13 primer pairs cross-amplified in Onychostoma sima. These microsatellite loci should prove highly informative for future studies of genetic diversity conservation.
SubtypeArticle
KeywordPseudogyrincheilus Prochilus Microsatellite Cross-species Amplification
DOI10.1007/s12686-009-9092-x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiodiversity & Conservation ; Genetics & Heredity
WOS SubjectBiodiversity Conservation ; Genetics & Heredity
WOS IDWOS:000207922400090
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27496
Collection水生生物多样性与资源保护研究中心
Affiliation1.Minist Water Resources, Inst Hydroecol, Wuhan 430079, Hubei, Peoples R China
2.Chinese Acad Sci, Wuhan 430079, Hubei, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Shi, Fang,Xu, Nian,Xiong, Meihua,et al. Isolation and characterization of 15 microsatellite loci in an endemic Chinese cyprinid fish, Pseudogyrincheilus prochilus, and their cross-species amplification in two related species[J]. CONSERVATION GENETICS RESOURCES,2009,1(1):397-399.
APA Shi, Fang.,Xu, Nian.,Xiong, Meihua.,Wang, Xiang.,Yang, Zhong.,...&Chang, Jianbo.(2009).Isolation and characterization of 15 microsatellite loci in an endemic Chinese cyprinid fish, Pseudogyrincheilus prochilus, and their cross-species amplification in two related species.CONSERVATION GENETICS RESOURCES,1(1),397-399.
MLA Shi, Fang,et al."Isolation and characterization of 15 microsatellite loci in an endemic Chinese cyprinid fish, Pseudogyrincheilus prochilus, and their cross-species amplification in two related species".CONSERVATION GENETICS RESOURCES 1.1(2009):397-399.
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