| Dietary leucine improves flesh quality and alters mRNA expressions of Nrf2-mediated antioxidant enzymes in the muscle of grass carp (Ctenopharyngodon idella) | |
| Deng, Yu-Ping1; Jiang, Wei-Dan1,2,3; Liu, Yang1,2,3; Qu, Biao1; Jiang, Jun1,2,3; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Wu, Pei1; Zhang, Yong-An5; Zhou, Xiao-Qiu1,2,3; Feng, Lin1,2,3 | |
| 2016-02-01 | |
| Source Publication | AQUACULTURE
 |
| ISSN | 0044-8486 |
| Volume | 452Pages:380-387 |
| Abstract | The objective of the study was to assess the influence of dietary leucine on flesh quality and the mRNA level of Nrf2-mediated antioxidant enzymes in the muscle of grass carp (Ctenopharyngodon idella). A total of 540 grass carp (295.85 +/- 2.07 g) were fed six diets with graded levels of leucine (7.1, 8.9, 11.0, 13.3, 15.2 and 17.1 g kg(-1) diet) for eight weeks. The result indicated that muscle protein, lipid content, muscle EAA (essential amino acid) levels, shear force, and hydroxyproline concentrationwere significantly improved by 11.0-17.1, 8.9-17.1, 8.9-17.1, 11.0-13.3, 8.9-13.3 g leucine kg(-1) diet supplementations, respectively. On the other hand, cooking loss, activities of cathepsin L, malondialdehyde and protein carbonyl contents exhibited an opposite tendency. Moreover, the results indicated that compared with leucine unsupplemented group (7.1 g leucine kg(-1) diet), 8.9-17.1, 11.0-17.1, 11.0-15.2 and 8.9-15.2 g kg(-1) leucine supplementations significantly enhanced the activities of copper/zinc superoxide dismutase (SOD1), catalase (CAT) and glutathione peroxidase (GPx), and glutathione content, respectively. Furthermore, the changes in the relative mRNA expressions of SOD1, CAT, GPx, nuclear factor erythroid 2-related factor 2 (Nrf2), target of rapamycin (TOR), and ribosomal protein S6 kinase beta-1 (S6K1) induced by leucine were similar to those noted in their enzyme activities. Conversely, the mRNA levels of Kelch-like ECH-associated protein 1 (Keap1) were significantly down-regulated by leucine supplementation. In conclusion, optimum leucine improved flesh quality, partly due to the enhancement of antioxidant capacity through regulating antioxidant-related signaling molecule. Based on muscle MDA, the optimum dietary leucine requirements of young grass carp (296-690 g) for flesh quality were estimated to be 12.8 g kg(-1) diet, corresponding to 39.3 g kg(-1) of dietary protein. (C) 2015 Elsevier B.V. All rights reserved. |
| Subtype | Article |
| Keyword | Grass Carp (Ctenopharyngodon Idella) Leucine Flesh Quality Antioxidant Enzyme Gene Expression Nrf2 |
| DOI | 10.1016/j.aquaculture.2015.11.007 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045) |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Fisheries ; Marine & Freshwater Biology |
| WOS Subject | Fisheries ; Marine & Freshwater Biology |
| WOS ID | WOS:000366205900047 |
| WOS Keyword | SALMON SALMO-SALAR ; BREAM SPARUS-AURATA ; GENE-EXPRESSION ; GROWTH-PERFORMANCE ; OXIDATIVE STRESS ; ARGININE SUPPLEMENTATION ; RELATIVE EXPRESSION ; JUVENILE JIAN ; GRADED-LEVELS ; MEAT QUALITY |
| Funding Organization | National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045) |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/27451 |
| Collection | 水生生物分子与细胞生物学研究中心_期刊论文 |
| Affiliation | 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China 2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China 3.Sichuan Agr Univ, Key Lab Anim Dis Resistance Nutr, China Minist Educ, Chengdu 611130, Sichuan, Peoples R China 4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 611130, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Deng, Yu-Ping,Jiang, Wei-Dan,Liu, Yang,et al. Dietary leucine improves flesh quality and alters mRNA expressions of Nrf2-mediated antioxidant enzymes in the muscle of grass carp (Ctenopharyngodon idella)[J]. AQUACULTURE,2016,452:380-387. |
| APA | Deng, Yu-Ping.,Jiang, Wei-Dan.,Liu, Yang.,Qu, Biao.,Jiang, Jun.,...&Feng, Lin.(2016).Dietary leucine improves flesh quality and alters mRNA expressions of Nrf2-mediated antioxidant enzymes in the muscle of grass carp (Ctenopharyngodon idella).AQUACULTURE,452,380-387. |
| MLA | Deng, Yu-Ping,et al."Dietary leucine improves flesh quality and alters mRNA expressions of Nrf2-mediated antioxidant enzymes in the muscle of grass carp (Ctenopharyngodon idella)".AQUACULTURE 452(2016):380-387. |
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