Dietary leucine improves flesh quality and alters mRNA expressions of Nrf2-mediated antioxidant enzymes in the muscle of grass carp (Ctenopharyngodon idella)
Deng, Yu-Ping1; Jiang, Wei-Dan1,2,3; Liu, Yang1,2,3; Qu, Biao1; Jiang, Jun1,2,3; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Wu, Pei1; Zhang, Yong-An5; Zhou, Xiao-Qiu1,2,3; Feng, Lin1,2,3
2016-02-01
Source PublicationAQUACULTURE
ISSN0044-8486
Volume452Pages:380-387
AbstractThe objective of the study was to assess the influence of dietary leucine on flesh quality and the mRNA level of Nrf2-mediated antioxidant enzymes in the muscle of grass carp (Ctenopharyngodon idella). A total of 540 grass carp (295.85 +/- 2.07 g) were fed six diets with graded levels of leucine (7.1, 8.9, 11.0, 13.3, 15.2 and 17.1 g kg(-1) diet) for eight weeks. The result indicated that muscle protein, lipid content, muscle EAA (essential amino acid) levels, shear force, and hydroxyproline concentrationwere significantly improved by 11.0-17.1, 8.9-17.1, 8.9-17.1, 11.0-13.3, 8.9-13.3 g leucine kg(-1) diet supplementations, respectively. On the other hand, cooking loss, activities of cathepsin L, malondialdehyde and protein carbonyl contents exhibited an opposite tendency. Moreover, the results indicated that compared with leucine unsupplemented group (7.1 g leucine kg(-1) diet), 8.9-17.1, 11.0-17.1, 11.0-15.2 and 8.9-15.2 g kg(-1) leucine supplementations significantly enhanced the activities of copper/zinc superoxide dismutase (SOD1), catalase (CAT) and glutathione peroxidase (GPx), and glutathione content, respectively. Furthermore, the changes in the relative mRNA expressions of SOD1, CAT, GPx, nuclear factor erythroid 2-related factor 2 (Nrf2), target of rapamycin (TOR), and ribosomal protein S6 kinase beta-1 (S6K1) induced by leucine were similar to those noted in their enzyme activities. Conversely, the mRNA levels of Kelch-like ECH-associated protein 1 (Keap1) were significantly down-regulated by leucine supplementation. In conclusion, optimum leucine improved flesh quality, partly due to the enhancement of antioxidant capacity through regulating antioxidant-related signaling molecule. Based on muscle MDA, the optimum dietary leucine requirements of young grass carp (296-690 g) for flesh quality were estimated to be 12.8 g kg(-1) diet, corresponding to 39.3 g kg(-1) of dietary protein. (C) 2015 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordGrass Carp (Ctenopharyngodon Idella) Leucine Flesh Quality Antioxidant Enzyme Gene Expression Nrf2
DOI10.1016/j.aquaculture.2015.11.007
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045)
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS KeywordSALMON SALMO-SALAR ; BREAM SPARUS-AURATA ; GENE-EXPRESSION ; GROWTH-PERFORMANCE ; OXIDATIVE STRESS ; ARGININE SUPPLEMENTATION ; RELATIVE EXPRESSION ; JUVENILE JIAN ; GRADED-LEVELS ; MEAT QUALITY
WOS IDWOS:000366205900047
Funding OrganizationNational Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045)
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27451
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, Key Lab Anim Dis Resistance Nutr, China Minist Educ, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 611130, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Deng, Yu-Ping,Jiang, Wei-Dan,Liu, Yang,et al. Dietary leucine improves flesh quality and alters mRNA expressions of Nrf2-mediated antioxidant enzymes in the muscle of grass carp (Ctenopharyngodon idella)[J]. AQUACULTURE,2016,452:380-387.
APA Deng, Yu-Ping.,Jiang, Wei-Dan.,Liu, Yang.,Qu, Biao.,Jiang, Jun.,...&Feng, Lin.(2016).Dietary leucine improves flesh quality and alters mRNA expressions of Nrf2-mediated antioxidant enzymes in the muscle of grass carp (Ctenopharyngodon idella).AQUACULTURE,452,380-387.
MLA Deng, Yu-Ping,et al."Dietary leucine improves flesh quality and alters mRNA expressions of Nrf2-mediated antioxidant enzymes in the muscle of grass carp (Ctenopharyngodon idella)".AQUACULTURE 452(2016):380-387.
Files in This Item:
File Name/Size DocType Version Access License
Dietary leucine impr(518KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Deng, Yu-Ping]'s Articles
[Jiang, Wei-Dan]'s Articles
[Liu, Yang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Deng, Yu-Ping]'s Articles
[Jiang, Wei-Dan]'s Articles
[Liu, Yang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Deng, Yu-Ping]'s Articles
[Jiang, Wei-Dan]'s Articles
[Liu, Yang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Dietary leucine improves flesh quality and alters mRNA expressions of Nrf2-mediated antioxidant enzymes in the muscle of grass carp (Ctenopharyngodon idella).pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.