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题名: DNA barcoding for the identification of common economic aquatic products in Central China and its application for the supervision of the market trade
作者: Shen, Yanjun1, 2; Kang, Jingliang1, 2; Chen, Weitao1, 2; He, Shunping1
关键词: DNA barcoding ; Aquatic products ; Cytochrome c oxidase subunit I ; K2P distance ; Identification
刊名: FOOD CONTROL
发表日期: 2016-03-01
DOI: 10.1016/j.foodcont.2015.08.038
卷: 61, 页:79-91
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Food Science & Technology
研究领域[WOS]: Food Science & Technology
英文摘要: Common economic aquatic products are important contributors to the human food supply. However, with the rapid globalization of the aquatic products industry, aquatic products market has become increasingly disordered. Therefore, an accurate and convenient method for identifying common economic aquatic products is important and necessary in many areas. DNA barcoding, which constitutes the analysis of a short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) sequence, has been widely used in species identification. To discriminate common economic aquatic species using DNA barcoding, we collected 534 COI barcode sequences of 66 common species consisting of 39 fish, 9 crustaceans and 18 mollusks. The intraspecific genetic distances based on the Kimura 2-parameter (K2P) model were less than 1.37% for fish, 7.32% for crustaceans and 3.40% for mollusks, whereas the intragenus distances ranged from 3.91% to 13.82% for fish, 14.99%-16.17% for crustaceans and 14.82%-1636% for mollusks. The average intraspecific K2P distance was also compared with the average intragenus distance. The taxonomic resolution ratio values obtained for fish, crustaceans and mollusks were 58.50, 21.59 and 27.63 respectively, which are higher than the threshold of (10 x). A neighbor-joining (NJ) tree based on the K2P distance, and a maximum likelihood (ML) tree, based on the GTR + I + G model, were constructed, and all of the species could be identified unambiguously in the trees with several branches exhibiting high bootstrap values. Our results demonstrated high efficiency of DNA barcoding as an efficient molecular tool for the identification of common economic aquatic products, and 8 substitute species were successfully detected in 66 species. Our analyses also indicated that the common aquatic products trade industry could be effectively monitored and managed by DNA barcoding. Therefore, a simple identification database of common economic aquatic products was constructed. (C) 2015 Published by Elsevier Ltd.
关键词[WOS]: OXIDASE SUBUNIT-I ; FISH PRODUCTS ; BIOLOGICAL IDENTIFICATIONS ; MAXIMUM-LIKELIHOOD ; SEAFOOD PRODUCTS ; COMMERCIAL FISH ; SUBSTITUTION ; REVEALS ; SEQUENCES ; CONSERVATION
语种: 英语
项目资助者: Ministry of Science and Technology of China(2012FY111300) ; Key Lab of Freshwater Fish Germplasm and Biotechnology, Ministry of Agriculture
WOS记录号: WOS:000365360100011
ISSN号: 0956-7135
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27428
Appears in Collections:水生生物多样性与资源保护研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
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