IHB OpenIR  > 水生生物多样性与资源保护研究中心  > 期刊论文
DNA barcoding for the identification of common economic aquatic products in Central China and its application for the supervision of the market trade
Shen, Yanjun1,2; Kang, Jingliang1,2; Chen, Weitao1,2; He, Shunping1
2016-03-01
Source PublicationFOOD CONTROL
ISSN0956-7135
Volume61Pages:79-91
AbstractCommon economic aquatic products are important contributors to the human food supply. However, with the rapid globalization of the aquatic products industry, aquatic products market has become increasingly disordered. Therefore, an accurate and convenient method for identifying common economic aquatic products is important and necessary in many areas. DNA barcoding, which constitutes the analysis of a short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) sequence, has been widely used in species identification. To discriminate common economic aquatic species using DNA barcoding, we collected 534 COI barcode sequences of 66 common species consisting of 39 fish, 9 crustaceans and 18 mollusks. The intraspecific genetic distances based on the Kimura 2-parameter (K2P) model were less than 1.37% for fish, 7.32% for crustaceans and 3.40% for mollusks, whereas the intragenus distances ranged from 3.91% to 13.82% for fish, 14.99%-16.17% for crustaceans and 14.82%-1636% for mollusks. The average intraspecific K2P distance was also compared with the average intragenus distance. The taxonomic resolution ratio values obtained for fish, crustaceans and mollusks were 58.50, 21.59 and 27.63 respectively, which are higher than the threshold of (10 x). A neighbor-joining (NJ) tree based on the K2P distance, and a maximum likelihood (ML) tree, based on the GTR + I + G model, were constructed, and all of the species could be identified unambiguously in the trees with several branches exhibiting high bootstrap values. Our results demonstrated high efficiency of DNA barcoding as an efficient molecular tool for the identification of common economic aquatic products, and 8 substitute species were successfully detected in 66 species. Our analyses also indicated that the common aquatic products trade industry could be effectively monitored and managed by DNA barcoding. Therefore, a simple identification database of common economic aquatic products was constructed. (C) 2015 Published by Elsevier Ltd.
SubtypeArticle
KeywordDna Barcoding Aquatic Products Cytochrome c Oxidase Subunit i K2p Distance Identification
DOI10.1016/j.foodcont.2015.08.038
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationMinistry of Science and Technology of China(2012FY111300) ; Ministry of Science and Technology of China(2012FY111300) ; Key Lab of Freshwater Fish Germplasm and Biotechnology, Ministry of Agriculture ; Key Lab of Freshwater Fish Germplasm and Biotechnology, Ministry of Agriculture ; Ministry of Science and Technology of China(2012FY111300) ; Ministry of Science and Technology of China(2012FY111300) ; Key Lab of Freshwater Fish Germplasm and Biotechnology, Ministry of Agriculture ; Key Lab of Freshwater Fish Germplasm and Biotechnology, Ministry of Agriculture
Indexed BySCI
Language英语
WOS Research AreaFood Science & Technology
WOS SubjectFood Science & Technology
WOS IDWOS:000365360100011
WOS KeywordOXIDASE SUBUNIT-I ; FISH PRODUCTS ; BIOLOGICAL IDENTIFICATIONS ; MAXIMUM-LIKELIHOOD ; SEAFOOD PRODUCTS ; COMMERCIAL FISH ; SUBSTITUTION ; REVEALS ; SEQUENCES ; CONSERVATION
Funding OrganizationMinistry of Science and Technology of China(2012FY111300) ; Ministry of Science and Technology of China(2012FY111300) ; Key Lab of Freshwater Fish Germplasm and Biotechnology, Ministry of Agriculture ; Key Lab of Freshwater Fish Germplasm and Biotechnology, Ministry of Agriculture ; Ministry of Science and Technology of China(2012FY111300) ; Ministry of Science and Technology of China(2012FY111300) ; Key Lab of Freshwater Fish Germplasm and Biotechnology, Ministry of Agriculture ; Key Lab of Freshwater Fish Germplasm and Biotechnology, Ministry of Agriculture
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27428
Collection水生生物多样性与资源保护研究中心_期刊论文
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Shen, Yanjun,Kang, Jingliang,Chen, Weitao,et al. DNA barcoding for the identification of common economic aquatic products in Central China and its application for the supervision of the market trade[J]. FOOD CONTROL,2016,61:79-91.
APA Shen, Yanjun,Kang, Jingliang,Chen, Weitao,&He, Shunping.(2016).DNA barcoding for the identification of common economic aquatic products in Central China and its application for the supervision of the market trade.FOOD CONTROL,61,79-91.
MLA Shen, Yanjun,et al."DNA barcoding for the identification of common economic aquatic products in Central China and its application for the supervision of the market trade".FOOD CONTROL 61(2016):79-91.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Shen, Yanjun]'s Articles
[Kang, Jingliang]'s Articles
[Chen, Weitao]'s Articles
Baidu academic
Similar articles in Baidu academic
[Shen, Yanjun]'s Articles
[Kang, Jingliang]'s Articles
[Chen, Weitao]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Shen, Yanjun]'s Articles
[Kang, Jingliang]'s Articles
[Chen, Weitao]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.