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Quantitatively evaluating detoxification of the hepatotoxic microcystin-LR through the glutathione (GSH) pathway in SD rats
Guo, Xiaochun1,2; Chen, Liang2,3; Chen, Jun2; Xie, Ping1,2; Li, Shangchun2,3; He, Jun2; Li, Wei2,3; Fan, Huihui1,2; Yu, Dezhao2,3; Zeng, Cheng1,2
2015-12-01
Source PublicationENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
ISSN0944-1344
Volume22Issue:23Pages:19273-19284
Abstract

Glutathione (GSH) plays crucial roles in antioxidant defense and detoxification metabolism of microcystin-LR (MC-LR). However, the detoxification process of MC-LR in mammals remains largely unknown. This paper, for the first time, quantitatively analyzes MC-LR and its GSH pathway metabolites (MC-LR-GSH and MC-LR-Cys) in the liver of Sprague-Dawley (SD) rat after MC-LR exposure. Rats received intraperitoneal (i.p.) injection of 0.25 and 0.5 lethal dose 50 (LD50) of MC-LR with or without pretreatment of buthionine-(S,R)-sulfoximine (BSO), an inhibitor of GSH synthesis. The contents of MC-LR-GSH were relatively low during the experiment; however, the ratio of MC-LR-Cys to MC-LR reached as high as 6.65 in 0.5 LD50 group. These results demonstrated that MC-LR-GSH could be converted to MC-LR-Cys efficiently, and this metabolic rule was in agreement with the data of aquatic animals previously reported. MC-LR contents were much higher in BSO + MC-LR-treated groups than in the single MC-LR-treated groups. Moreover, the ratio of MC-LR-Cys to MC-LR decreased significantly after BSO pretreatment, suggesting that the depletion of GSH induced by BSO reduced the detoxification of MCs. Moreover, MC-LR remarkably induced liver damage, and the effects were more pronounced in BSO pretreatment groups. In conclusion, this study verifies the role of GSH in the detoxification of MC-LR and furthers our understanding of the biochemical mechanism for SD rats to counteract toxic cyanobacteria.

SubtypeArticle
KeywordMicrocystin-lr-gsh/cys Detoxification Gsh Pathway Liver Rat
DOI10.1007/s11356-015-5531-2
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; 31322013) ; 31322013) ; 31322013) ; 31322013) ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; 31322013) ; 31322013) ; 31322013) ; 31322013) ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; 31322013) ; 31322013) ; 31322013) ; 31322013) ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; 31322013) ; 31322013) ; 31322013) ; 31322013)
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000365816000090
WOS KeywordCARP HYPOPHTHALMICHTHYS-MOLITRIX ; TANDEM MASS-SPECTROMETRY ; CYCLIC PEPTIDE TOXIN ; BIGHEAD CARP ; INHIBITORY-ACTIVITY ; OXIDATIVE STRESS ; BALB/C MICE ; IN-VIVO ; LIVER ; TOXICITY
Funding OrganizationNational Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; 31322013) ; 31322013) ; 31322013) ; 31322013) ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; 31322013) ; 31322013) ; 31322013) ; 31322013) ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; 31322013) ; 31322013) ; 31322013) ; 31322013) ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; National Nature Science Foundation of China(31070457 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; 31322013) ; 31322013) ; 31322013) ; 31322013)
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27425
Collection淡水生态学研究中心_期刊论文
Affiliation1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China
2.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Guo, Xiaochun,Chen, Liang,Chen, Jun,et al. Quantitatively evaluating detoxification of the hepatotoxic microcystin-LR through the glutathione (GSH) pathway in SD rats[J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,2015,22(23):19273-19284.
APA Guo, Xiaochun.,Chen, Liang.,Chen, Jun.,Xie, Ping.,Li, Shangchun.,...&Zeng, Cheng.(2015).Quantitatively evaluating detoxification of the hepatotoxic microcystin-LR through the glutathione (GSH) pathway in SD rats.ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,22(23),19273-19284.
MLA Guo, Xiaochun,et al."Quantitatively evaluating detoxification of the hepatotoxic microcystin-LR through the glutathione (GSH) pathway in SD rats".ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 22.23(2015):19273-19284.
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