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题名: Quantitatively evaluating detoxification of the hepatotoxic microcystin-LR through the glutathione (GSH) pathway in SD rats
作者: Guo, Xiaochun1, 2; Chen, Liang2, 3; Chen, Jun2; Xie, Ping1, 2; Li, Shangchun2, 3; He, Jun2; Li, Wei2, 3; Fan, Huihui1, 2; Yu, Dezhao2, 3; Zeng, Cheng1, 2
关键词: Microcystin-LR-GSH/Cys ; Detoxification ; GSH pathway ; Liver ; Rat
刊名: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
发表日期: 2015-12-01
DOI: 10.1007/s11356-015-5531-2
卷: 22, 期:23, 页:19273-19284
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Environmental Sciences
研究领域[WOS]: Environmental Sciences & Ecology
英文摘要: Glutathione (GSH) plays crucial roles in antioxidant defense and detoxification metabolism of microcystin-LR (MC-LR). However, the detoxification process of MC-LR in mammals remains largely unknown. This paper, for the first time, quantitatively analyzes MC-LR and its GSH pathway metabolites (MC-LR-GSH and MC-LR-Cys) in the liver of Sprague-Dawley (SD) rat after MC-LR exposure. Rats received intraperitoneal (i.p.) injection of 0.25 and 0.5 lethal dose 50 (LD50) of MC-LR with or without pretreatment of buthionine-(S,R)-sulfoximine (BSO), an inhibitor of GSH synthesis. The contents of MC-LR-GSH were relatively low during the experiment; however, the ratio of MC-LR-Cys to MC-LR reached as high as 6.65 in 0.5 LD50 group. These results demonstrated that MC-LR-GSH could be converted to MC-LR-Cys efficiently, and this metabolic rule was in agreement with the data of aquatic animals previously reported. MC-LR contents were much higher in BSO + MC-LR-treated groups than in the single MC-LR-treated groups. Moreover, the ratio of MC-LR-Cys to MC-LR decreased significantly after BSO pretreatment, suggesting that the depletion of GSH induced by BSO reduced the detoxification of MCs. Moreover, MC-LR remarkably induced liver damage, and the effects were more pronounced in BSO pretreatment groups. In conclusion, this study verifies the role of GSH in the detoxification of MC-LR and furthers our understanding of the biochemical mechanism for SD rats to counteract toxic cyanobacteria.
关键词[WOS]: CARP HYPOPHTHALMICHTHYS-MOLITRIX ; TANDEM MASS-SPECTROMETRY ; CYCLIC PEPTIDE TOXIN ; BIGHEAD CARP ; INHIBITORY-ACTIVITY ; OXIDATIVE STRESS ; BALB/C MICE ; IN-VIVO ; LIVER ; TOXICITY
语种: 英语
项目资助者: National Nature Science Foundation of China(31070457 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02) ; 31322013)
WOS记录号: WOS:000365816000090
ISSN号: 0944-1344
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27425
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China
2.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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