IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Dietary pantothenic acid depressed the gill immune and physical barrier function via NF-kappa B, TOR, Nrf2, p38MAPK and MLCK signaling pathways in grass carp (Ctenopharyngodon idella)
Li, Li1; Feng, Lin1,2,3; Jiang, Wei-Dan1,2,3; Jiang, Jun1,2,3; Wu, Pei1,2,3; Zhao, Juan1; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5; Zhou, Xiao-Qiu1,2,3; Liu, Yang1,2,3
2015-11-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume47Issue:1Pages:500-510
AbstractThis study explored the effects of pantothenic acid (PA) on the immune and physical barrier function, and relative mRNA levels of signaling molecules in the gill of grass carp (Ctenophatyngodon idella). The results indicated that compared with optimal PA supplementation, PA deficiency (1.31 mg/kg diet) decreased gill interleukin 10, transforming growth factor beta 1, inhibitor of kappa B alpha (I kappa B alpha), eIF4E-binding protein 2, Claudin b and ZO-1 mRNA levels; anti-superoxide anion activity, and activities and mRNA levels of copper/zinc superoxide dismutase, manganese superoxide dismutase, glutathione peroxidase, glutathione reductase and NF-E2-related factor (P < 0.05). Additionally, PA deficiency and excess (75.08 mg/kg diet) decreased gill complement 3 and glutathione contents, lysozyme and acid phosphatase, anti-hydroxy radical, catalase and glutathione S-transferases activities, and liver-expression antimicrobial peptide 2, hepcidin, Claudin 3, Claudin c and Occludin mRNA levels (P < 0.05). Conversely, PA deficiency increased gill reactive oxygen species and protein carbonyl contents, and interferon gamma 2, interleukin 8, nuclear factor kappa B P65, Claudin 15a, Kelch-like ECH-associating protein 1a and Kelch-like ECH-associating protein 1b mRNA levels (P <0.05). Moreover, PA deficiency and excess increased gill malondialdehyde content, and tumor necrosis factor alpha, interleukin 1 beta, I kappa B kinase alpha, I kappa B kinase beta, I kappa B kinase gamma, target of rapamycin and ribosomal S6 protein kinase1 p38 mitogen-activated protein kinases and myosin light-chain kinase mRNA levels (P < 0.05). In conclusion, PA deficiency decreased immune and physical barrier function, and regulated relative mRNA levels of signaling molecules in fish gill. Based on the quadratic regression analysis of gill lysozyme activity, the optimal PA levels in grass carp (253.44-745.25 g) were estimated to be 36.97 mg/kg diet. (c) 2015 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordPantothenic Acid Grass Carp Immune Physical Barrier Antioxidant Enzyme Tight Junction Protein Mrna Level Gill
DOI10.1016/j.fsi.2015.09.038
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; 2013NC0045) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; 2013NC0045) ; 2013NC0045)
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000365053300056
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; TIGHT JUNCTION PROTEINS ; TILAPIA OREOCHROMIS-NILOTICUS ; INTESTINAL MUCOSAL IMMUNE ; SEABREAM SPARUS-AURATA ; GENE-EXPRESSION ; ANTIOXIDANT STATUS ; GROWTH-PERFORMANCE ; ENZYME-ACTIVITIES ; JUVENILE JIAN
Funding OrganizationNational Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; 2013NC0045) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; 2013NC0045) ; 2013NC0045)
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Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27403
Collection水生生物分子与细胞生物学研究中心_期刊论文
Affiliation1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Sichuan, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Li, Li,Feng, Lin,Jiang, Wei-Dan,et al. Dietary pantothenic acid depressed the gill immune and physical barrier function via NF-kappa B, TOR, Nrf2, p38MAPK and MLCK signaling pathways in grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2015,47(1):500-510.
APA Li, Li.,Feng, Lin.,Jiang, Wei-Dan.,Jiang, Jun.,Wu, Pei.,...&Liu, Yang.(2015).Dietary pantothenic acid depressed the gill immune and physical barrier function via NF-kappa B, TOR, Nrf2, p38MAPK and MLCK signaling pathways in grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,47(1),500-510.
MLA Li, Li,et al."Dietary pantothenic acid depressed the gill immune and physical barrier function via NF-kappa B, TOR, Nrf2, p38MAPK and MLCK signaling pathways in grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 47.1(2015):500-510.
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