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题名: Dietary pantothenic acid depressed the gill immune and physical barrier function via NF-kappa B, TOR, Nrf2, p38MAPK and MLCK signaling pathways in grass carp (Ctenopharyngodon idella)
作者: Li, Li1; Feng, Lin1, 2, 3; Jiang, Wei-Dan1, 2, 3; Jiang, Jun1, 2, 3; Wu, Pei1, 2, 3; Zhao, Juan1; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5; Zhou, Xiao-Qiu1, 2, 3; Liu, Yang1, 2, 3
关键词: Pantothenic acid ; Grass carp ; Immune ; Physical barrier ; Antioxidant enzyme ; Tight junction protein ; mRNA level ; Gill
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2015-11-01
DOI: 10.1016/j.fsi.2015.09.038
卷: 47, 期:1, 页:500-510
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
英文摘要: This study explored the effects of pantothenic acid (PA) on the immune and physical barrier function, and relative mRNA levels of signaling molecules in the gill of grass carp (Ctenophatyngodon idella). The results indicated that compared with optimal PA supplementation, PA deficiency (1.31 mg/kg diet) decreased gill interleukin 10, transforming growth factor beta 1, inhibitor of kappa B alpha (I kappa B alpha), eIF4E-binding protein 2, Claudin b and ZO-1 mRNA levels; anti-superoxide anion activity, and activities and mRNA levels of copper/zinc superoxide dismutase, manganese superoxide dismutase, glutathione peroxidase, glutathione reductase and NF-E2-related factor (P < 0.05). Additionally, PA deficiency and excess (75.08 mg/kg diet) decreased gill complement 3 and glutathione contents, lysozyme and acid phosphatase, anti-hydroxy radical, catalase and glutathione S-transferases activities, and liver-expression antimicrobial peptide 2, hepcidin, Claudin 3, Claudin c and Occludin mRNA levels (P < 0.05). Conversely, PA deficiency increased gill reactive oxygen species and protein carbonyl contents, and interferon gamma 2, interleukin 8, nuclear factor kappa B P65, Claudin 15a, Kelch-like ECH-associating protein 1a and Kelch-like ECH-associating protein 1b mRNA levels (P <0.05). Moreover, PA deficiency and excess increased gill malondialdehyde content, and tumor necrosis factor alpha, interleukin 1 beta, I kappa B kinase alpha, I kappa B kinase beta, I kappa B kinase gamma, target of rapamycin and ribosomal S6 protein kinase1 p38 mitogen-activated protein kinases and myosin light-chain kinase mRNA levels (P < 0.05). In conclusion, PA deficiency decreased immune and physical barrier function, and regulated relative mRNA levels of signaling molecules in fish gill. Based on the quadratic regression analysis of gill lysozyme activity, the optimal PA levels in grass carp (253.44-745.25 g) were estimated to be 36.97 mg/kg diet. (c) 2015 Elsevier Ltd. All rights reserved.
关键词[WOS]: TROUT ONCORHYNCHUS-MYKISS ; TIGHT JUNCTION PROTEINS ; TILAPIA OREOCHROMIS-NILOTICUS ; INTESTINAL MUCOSAL IMMUNE ; SEABREAM SPARUS-AURATA ; GENE-EXPRESSION ; ANTIOXIDANT STATUS ; GROWTH-PERFORMANCE ; ENZYME-ACTIVITIES ; JUVENILE JIAN
语种: 英语
项目资助者: National Basic Research Program of China (973 Program)(2014CB138600) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; 2013NC0045)
WOS记录号: WOS:000365053300056
ISSN号: 1050-4648
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27403
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Sichuan, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
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