| Transcriptomic analysis of the host response to an iridovirus infection in Chinese giant salamander, Andrias davidianus | |
| Fan, Yuding1,4; Chang, Ming Xian2; Ma, Jie1; LaPatra, Scott E.3; Hu, Yi Wei2; Huang, Lili1; Nie, Pin2; Zeng, Lingbing1 | |
| 2015-11-20 | |
| Source Publication | VETERINARY RESEARCH
 |
| ISSN | 0928-4249 |
| Volume | 46 |
| Abstract | The emergence of an infectious viral disease caused by the Chinese giant salamander iridovirus (GSIV) has led to substantial economic losses. However, no more molecular information is available for the understanding of the mechanisms associated with virus-host interaction. In this study, de novo sequencing was used to obtain abundant high-quality ESTs and investigate differentially-expressed genes in the spleen of Chinese giant salamanders that were either infected or mock infected with GSIV. Comparative expression analysis indicated that 293 genes were down-regulated and 220 genes were up-regulated. Further enrichment analysis showed that the most enriched pathway is "complement and coagulation cascades", and significantly enriched diseases include "inherited thrombophilia", "immune system diseases", "primary immunodeficiency", "complement regulatory protein defects", and "disorders of nucleotide excision repair". Additionally, 30 678 simple sequence repeats (SSRs) from all spleen samples, 26 355 single nucleotide polymorphisms (SNPs) from the spleens of uninfected animals and 36 070 SNPs from the spleens of infected animals were detected. The large amount of variation was specific for the Chinese giant salamanders that were infected with GSIV. The results reported herein provided significant and new EST information that could contribute greatly in investigations into the molecular functions of immune genes in the Chinese giant salamander. |
| Subtype | Article |
| DOI | 10.1186/s13567-015-0279-8 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | Special Fund for Agro-scientific Research in the Public Interest(201203086) ; Special Fund for Agro-scientific Research in the Public Interest(201203086) ; Special Fund for Agro-scientific Research in the Public Interest(201203086) ; Special Fund for Agro-scientific Research in the Public Interest(201203086) |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Veterinary Sciences |
| WOS Subject | Veterinary Sciences |
| WOS ID | WOS:000365161200003 |
| WOS Keyword | TOLL-LIKE RECEPTORS ; NOD-LIKE RECEPTORS ; IMMUNE GENE REPERTOIRE ; RNA-SEQ DATA ; INNATE IMMUNITY ; FUNCTIONAL-CHARACTERIZATION ; REFERENCE GENOME ; CHANNEL CATFISH ; SPLICE VARIANTS ; EXPRESSION |
| Funding Organization | Special Fund for Agro-scientific Research in the Public Interest(201203086) ; Special Fund for Agro-scientific Research in the Public Interest(201203086) ; Special Fund for Agro-scientific Research in the Public Interest(201203086) ; Special Fund for Agro-scientific Research in the Public Interest(201203086) |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/27399 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Affiliation | 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 3.Clear Springs Foods Inc, Div Res, Buhl, ID 83316 USA 4.Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430223, Hubei, Peoples R China |
| Recommended Citation GB/T 7714 | Fan, Yuding,Chang, Ming Xian,Ma, Jie,et al. Transcriptomic analysis of the host response to an iridovirus infection in Chinese giant salamander, Andrias davidianus[J]. VETERINARY RESEARCH,2015,46. |
| APA | Fan, Yuding.,Chang, Ming Xian.,Ma, Jie.,LaPatra, Scott E..,Hu, Yi Wei.,...&Zeng, Lingbing.(2015).Transcriptomic analysis of the host response to an iridovirus infection in Chinese giant salamander, Andrias davidianus.VETERINARY RESEARCH,46. |
| MLA | Fan, Yuding,et al."Transcriptomic analysis of the host response to an iridovirus infection in Chinese giant salamander, Andrias davidianus".VETERINARY RESEARCH 46(2015). |
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