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题名: Unique diversity of the venom peptides from the scorpion Androctonus bicolor revealed by transcriptomic and proteomic analysis
作者: Zhang, Lei1, 2; Shi, Wanxia1, 2; Zeng, Xian-Chun1, 2; Ge, Feng3; Yang, Mingkun3; Nie, Yao1, 2; Bao, Aorigele1, 2; Wu, Shifen1, 2; E, Guoji1, 2
关键词: Scorpion toxin ; Androctonus bicolor ; Venom peptide ; Transcriptomic and proteomic analysis ; Venom composition ; Ion channels
刊名: JOURNAL OF PROTEOMICS
发表日期: 2015-10-14
DOI: 10.1016/j.jprot.2015.07.030
卷: 128, 页:231-250
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Androctonus bicolor is one of the most poisonous scorpion species in the world. However, little has been known about the venom composition of the scorpion. To better understand the molecular diversity and medical significance of the venom from the scorpion, we systematically analyzed the venom components by combining transcriptomic and proteomic surveys. Random sequencing of 1000 clones from a cDNA library prepared from the venom glands of the scorpion revealed that 70% of the total transcripts code for venom peptide precursors. Our efforts led to a discovery of 103 novel putative venom peptides. These peptides include NaTx-like, ICTx-like and CaTx-like peptides, putative antimicrobial peptides, defensin-like peptides, BPP-like peptides, BmKa2-like peptides, Kunitz-type toxins and some new-type venom peptides without disulfide bridges, as well as many new-type venom peptides that are cross-linked with one, two, three, five or six disulfide bridges, respectively. We also identified three peptides that are identical to known toxins from scorpions. The venom was also analyzed using a proteomic technique. The presence of a total of 16 different venom peptides was confirmed by LC-MS/MS analysis. The discovery of a wide range of new and new-type venom peptides highlights the unique diversity of the venom peptides from A. bicolor. These data also provide a series of novel templates for the development of therapeutic drugs for treating ion channel-associated diseases and infections caused by antibiotic-resistant pathogens, and offer molecular probes for the exploration of structures and functions of various ion channels. (C) 2015 Elsevier B.V. All rights reserved.
关键词[WOS]: BUTHUS-MARTENSII KARSCH ; ANTI-EPILEPSY PEPTIDE ; MESOBUTHUS-MARTENSII ; MOLECULAR DIVERSITY ; PARABUTHUS-TRANSVAALICUS ; ANTIMICROBIAL PEPTIDES ; TITYUS-SERRULATUS ; CHANNEL TOXIN ; MASS-SPECTROMETRY ; GAINING INSIGHT
语种: 英语
项目资助者: China National 973 Research Project, Basic Research and Clinical Application of Venom Peptides from Toxic Animals(2010CB529800) ; Fundamental Research Funds for the Central Universities(CUGL100613 ; CUGL110604 ; CUGL120608)
WOS记录号: WOS:000364882200021
ISSN号: 1874-3919
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27395
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.China Univ Geosci, Dept Biol Sci & Technol, Sch Environm Studies, Wuhan 430074, Peoples R China
2.China Univ Geosci, State Key Lab Biogeol & Environm Geol, Wuhan 430074, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
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