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Effects of Phosphorylation of beta Subunits of Phycocyanins on State Transition in the Model Cyanobacterium Synechocystis sp PCC 6803
Chen, Zhuo1; Zhan, Jiao1,2; Chen, Ying1; Yang, Mingkun1; He, Chenliu1; Ge, Feng1; Wang, Qiang1
2015-10-01
Source PublicationPLANT AND CELL PHYSIOLOGY
ISSN0032-0781
Volume56Issue:10Pages:1997-2013
AbstractSynechocystis sp. PCC 6803 (hereafter Synechocystis) is a model cyanobacterium and has been used extensively for studies concerned with photosynthesis and environmental adaptation. Although dozens of protein kinases and phosphatases with specificity for Ser/Thr/Tyr residues have been predicted, only a few substrate proteins are known in Synechocystis. In this study, we report 194 in vivo phosphorylation sites from 149 proteins in Synechocystis, which were identified using a combination of peptide pre-fractionation, TiO2 enrichment and liquid chromatograpy-tandem mass spectrometry (LC-MS/MS) analysis. These phosphorylated proteins are implicated in diverse biological processes, such as photosynthesis. Among all identified phosphoproteins involved in photosynthesis, the beta subunits of phycocyanins (CpcBs) were found to be phosphorylated on Ser22, Ser49, Thr94 and Ser154. Four non-phosphorylated mutants were constructed by using site-directed mutagenesis. The in vivo characterization of the cpcB mutants showed a slower growth under high light irradiance and displayed fluorescence quenching to a lower level and less efficient energy transfer inside the phycobilisome (PBS). Notably, the non-phosphorylated mutants exhibited a slower state transition than the wild type. The current results demonstrated that the phosphorylation status of CpcBs affects the energy transfer and state transition of photosynthesis in Synechocystis. This study provides novel insights into the molecular mechanisms of protein phosphorylation in the regulation of photosynthesis in cyanobacteria and may facilitate the elucidation of the entire regulatory network by linking kinases to their physiological substrates.
SubtypeArticle
KeywordBeta Subunits Of Phycocyanins (Cpcbs) Phosphoproteomics Photosynthesis State Transition Synechocystis Sp Pcc 6803 Tio2 Enrichment
DOI10.1093/pcp/pcv118
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China [973 Program](2012CB518700) ; National Basic Research Program of China [973 Program](2012CB518700) ; National Natural Science Foundation of China(31270094 ; National Natural Science Foundation of China(31270094 ; Natural Science Foundation of Hubei Province of China(2013CFA109) ; Natural Science Foundation of Hubei Province of China(2013CFA109) ; 31370746) ; 31370746) ; National Basic Research Program of China [973 Program](2012CB518700) ; National Basic Research Program of China [973 Program](2012CB518700) ; National Natural Science Foundation of China(31270094 ; National Natural Science Foundation of China(31270094 ; Natural Science Foundation of Hubei Province of China(2013CFA109) ; Natural Science Foundation of Hubei Province of China(2013CFA109) ; 31370746) ; 31370746)
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Cell Biology
WOS SubjectPlant Sciences ; Cell Biology
WOS IDWOS:000364961600011
WOS KeywordSERINE/THREONINE PROTEIN-KINASES ; SP STRAIN PCC-6803 ; EXCITATION-ENERGY TRANSFER ; GRAM-NEGATIVE BACTERIUM ; PHOTOSYSTEM-I ; BACILLUS-SUBTILIS ; MYCOBACTERIUM-TUBERCULOSIS ; PHOSPHOPROTEOMIC ANALYSIS ; INTERACTION NETWORKS ; BLUE-GREEN
Funding OrganizationNational Basic Research Program of China [973 Program](2012CB518700) ; National Basic Research Program of China [973 Program](2012CB518700) ; National Natural Science Foundation of China(31270094 ; National Natural Science Foundation of China(31270094 ; Natural Science Foundation of Hubei Province of China(2013CFA109) ; Natural Science Foundation of Hubei Province of China(2013CFA109) ; 31370746) ; 31370746) ; National Basic Research Program of China [973 Program](2012CB518700) ; National Basic Research Program of China [973 Program](2012CB518700) ; National Natural Science Foundation of China(31270094 ; National Natural Science Foundation of China(31270094 ; Natural Science Foundation of Hubei Province of China(2013CFA109) ; Natural Science Foundation of Hubei Province of China(2013CFA109) ; 31370746) ; 31370746)
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Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27364
Collection水生生物分子与细胞生物学研究中心_期刊论文
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100094, Peoples R China
Recommended Citation
GB/T 7714
Chen, Zhuo,Zhan, Jiao,Chen, Ying,et al. Effects of Phosphorylation of beta Subunits of Phycocyanins on State Transition in the Model Cyanobacterium Synechocystis sp PCC 6803[J]. PLANT AND CELL PHYSIOLOGY,2015,56(10):1997-2013.
APA Chen, Zhuo.,Zhan, Jiao.,Chen, Ying.,Yang, Mingkun.,He, Chenliu.,...&Wang, Qiang.(2015).Effects of Phosphorylation of beta Subunits of Phycocyanins on State Transition in the Model Cyanobacterium Synechocystis sp PCC 6803.PLANT AND CELL PHYSIOLOGY,56(10),1997-2013.
MLA Chen, Zhuo,et al."Effects of Phosphorylation of beta Subunits of Phycocyanins on State Transition in the Model Cyanobacterium Synechocystis sp PCC 6803".PLANT AND CELL PHYSIOLOGY 56.10(2015):1997-2013.
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