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题名: Soyabean glycinin depresses intestinal growth and function in juvenile Jian carp (Cyprinus carpio var Jian): protective effects of glutamine
作者: Jiang, Wei-Dan1, 2, 3; Hu, Kai1, 6; Zhang, Jin-Xiu1; Liu, Yang1, 2, 3; Jiang, Jun1; Wu, Pei1, 2, 3; Zhao, Juan1; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5; Zhou, Xiao-Qiu1, 2, 3; Feng, Lin1, 2, 3
关键词: Glycinin ; Intestine ; Antioxidant ; Tight junction ; Cytokine ; Apoptosis
刊名: BRITISH JOURNAL OF NUTRITION
发表日期: 2015-11-28
DOI: 10.1017/S0007114515003219
卷: 114, 期:10, 页:1569-1583
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Nutrition & Dietetics
研究领域[WOS]: Nutrition & Dietetics
英文摘要: This study investigated the effects of glycinin on the growth, intestinal oxidative status, tight junction components, cytokines and apoptosis signalling factors of fish. The results showed that an 80 g/kg diet of glycinin exposure for 42 d caused poor growth performance and depressed intestinal growth and function of juvenile Jian carp (Cyprinus carpio var. Jian). Meanwhile, dietary glycinin exposure induced increases in lipid peroxidation and protein oxidation; it caused reductions in superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) activities; and it increased MnSOD, CuZnSOD, GPx1b and GPx4a mRNA levels, suggesting an adaptive mechanism against stress in the intestines of fish. However, dietary glycinin exposure decreased both the activity and mRNA levels of nine isoforms of glutathione-S-transferase (GST) (alpha, mu, pi, rho, theta, kappa, mGST1, mGST2 and mGST3), indicating toxicity to this enzyme activity and corresponding isoform gene expressions. In addition, glycinin exposure caused partial disruption of intestinal cell-cell tight junction components, disturbances of cytokines and induced apoptosis signalling in the distal intestines > mid intestines > proximal intestines of fish. Glycinin exposure also disturbed the mRNA levels of intestinal-related signalling factors Nrf2, Keap1a, Keap1b, eleven isoforms of protein kinase C and target of rapamycin/4E-BP. Interestingly, glutamine was observed to partially block those negative influences. In conclusion, this study indicates that dietary glycinin exposure causes intestinal oxidative damage and disruption of intestinal physical barriers and functions and reduces fish growth, but glutamine can reverse those negative effects in fish. This study provides some information on the mechanism of glycinin-induced negative effects.
关键词[WOS]: INDUCED OXIDATIVE STRESS ; SALMON SALMO-SALAR ; PROTEIN-KINASE-C ; ENZYME-ACTIVITIES ; BETA-CONGLYCININ ; GENE-EXPRESSION ; COPPER EXPOSURE ; RAINBOW-TROUT ; GRADED-LEVELS ; ANTIOXIDANT DEFENSES
语种: 英语
项目资助者: National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045)
WOS记录号: WOS:000364957900005
ISSN号: 0007-1145
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27361
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Peoples R China
3.Sichuan Agr Univ, Key Lab Anim Dis Resistance Nutr, China Minist Educ, Chengdu 611130, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
6.Chengdu Agr Coll, Dept Anim & Vet Sci, Chengdu 611130, Peoples R China
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