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Assessment of the Toxicity of CuO Nanoparticles by Using Saccharomyces cerevisiae Mutants with Multiple Genes Deleted
Bao, Shaopan1,2; Lu, Qicong1,2; Fang, Tao1; Dai, Heping1; Zhang, Chao1,2
2015-12-01
Source PublicationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
Volume81Issue:23Pages:8098-8107
AbstractTo develop applicable and susceptible models to evaluate the toxicity of nanoparticles, the antimicrobial effects of CuO nanoparticles (CuO-NPs) on various Saccharomyces cerevisiae (S. cerevisiae) strains (wild type, single-gene-deleted mutants, and multiple-gene-deleted mutants) were determined and compared. Further experiments were also conducted to analyze the mechanisms associated with toxicity using copper salt, bulk CuO (bCuO), carbon-shelled copper nanoparticles (C/Cu-NPs), and carbon nanoparticles (C-NPs) for comparisons. The results indicated that the growth inhibition rates of CuO-NPs for the wild-type and the single-gene-deleted strains were comparable, while for the multiple-gene deletion mutant, significantly higher toxicity was observed (P < 0.05). When the toxicity of the CuO-NPs to yeast cells was compared with the toxicities of copper salt and bCuO, we concluded that the toxicity of CuO-NPs should be attributed to soluble copper rather than to the nanoparticles. The striking difference in adverse effects of C-NPs and C/Cu-NPs with equivalent surface areas also proved this. A toxicity assay revealed that the multiple-gene-deleted mutant was significantly more sensitive to CuO-NPs than the wild type. Specifically, compared with the wild-type strain, copper was readily taken up by mutant strains when cell permeability genes were knocked out, and the mutants with deletions of genes regulated under oxidative stress (OS) were likely producing more reactive oxygen species (ROS). Hence, as mechanism-based gene inactivation could increase the susceptibility of yeast, the multiple-gene-deleted mutants should be improved model organisms to investigate the toxicity of nanoparticles.
SubtypeArticle
DOI10.1128/AEM.02035-15
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNatural Science Foundation of China(21477159 ; Natural Science Foundation of China(21477159 ; 21037004) ; 21037004) ; Natural Science Foundation of China(21477159 ; Natural Science Foundation of China(21477159 ; 21037004) ; 21037004)
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000364775700014
WOS KeywordMETAL-OXIDE NANOPARTICLES ; DISSOLVED ORGANIC-MATTER ; CELLS IN-VITRO ; OXIDATIVE STRESS ; CARBON NANOTUBES ; GENOTOXIC AGENTS ; PLASMA-MEMBRANE ; TESTING SYSTEM ; CWP GENES ; YEAST
Funding OrganizationNatural Science Foundation of China(21477159 ; Natural Science Foundation of China(21477159 ; 21037004) ; 21037004) ; Natural Science Foundation of China(21477159 ; Natural Science Foundation of China(21477159 ; 21037004) ; 21037004)
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Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27359
Collection水生生物分子与细胞生物学研究中心_期刊论文
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Bao, Shaopan,Lu, Qicong,Fang, Tao,et al. Assessment of the Toxicity of CuO Nanoparticles by Using Saccharomyces cerevisiae Mutants with Multiple Genes Deleted[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2015,81(23):8098-8107.
APA Bao, Shaopan,Lu, Qicong,Fang, Tao,Dai, Heping,&Zhang, Chao.(2015).Assessment of the Toxicity of CuO Nanoparticles by Using Saccharomyces cerevisiae Mutants with Multiple Genes Deleted.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,81(23),8098-8107.
MLA Bao, Shaopan,et al."Assessment of the Toxicity of CuO Nanoparticles by Using Saccharomyces cerevisiae Mutants with Multiple Genes Deleted".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 81.23(2015):8098-8107.
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