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The Role of Glutathione Detoxification Pathway in MCLR-Induced Hepatotoxicity in SD Rats
Li, Shangchun1,2; Chen, Jun1; Xie, Ping1; Guo, Xiaochun1,3; Fan, Huihui1,3; Yu, Dezhao1,2; Zeng, Cheng1,3; Chen, Liang1,2
2015-12-01
Source PublicationENVIRONMENTAL TOXICOLOGY
ISSN1520-4081
Volume30Issue:12Pages:1470-1480
AbstractIn the present study, we investigated the role of glutathione (GSH) and its related enzymes in Sprague Dawley (SD) rats subjected to microcystin-leucine-arginine (MCLR)-induced hepatotoxicity. SD rats were intraperitoneally (i.p.) injected with MCLR after pretreating with or without buthionine-(S,R)-sulfoximine (BSO), an inhibitor of GSH synthesis. The depletion of GSH with BSO enhanced MCLR-induced oxidative stress, resulting in more severe liver damage and higher MCLR accumulation. Similarly, the contents of malondialdehyde (MDA), total GSH (T-GSH), oxidized GSH (GSSG) and GSH were significantly enhanced in BSO pretreated rats following MCLR treatment. The study showed that the transcription of GSH-related enzymes such as glutathione-S-transferase (GST), gamma-glutamylcysteine synthetase (gamma-GCS), glutathione reductase (GR) varied in different ways (expect for glutathione peroxidase (GPx), whose gene expression was induced in all treated groups) with or without BSO pretreatment before MCLR exposure, suggesting an adaptative response of GSH-related enzymes at transcription level to combat enhancement of oxidative stress induced by MCLR when pretreated with BSO. These data suggested the tissues with low GSH concentration are highly vulnerable to MCLR toxicity and GSH was critical for the detoxification in MCLR-induced hepatotoxicity in vivo. (C) 2014 Wiley Periodicals, Inc.
SubtypeArticle
KeywordMicrocystin-lr Glutathione Oxidative Stress Liver Bso Sd Rat
DOI10.1002/tox.22017
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Funding OrganizationNational Natural Science Foundations of China ; National Natural Science Foundations of China ; (31070457) ; (31070457) ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; (31070457) ; (31070457)
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology ; Water Resources
WOS SubjectEnvironmental Sciences ; Toxicology ; Water Resources
WOS IDWOS:000364781900012
WOS KeywordINDUCED DNA-DAMAGE ; MICROCYSTIN-LR ; OXIDATIVE STRESS ; CYANOBACTERIAL TOXINS ; ANTIOXIDANT ENZYMES ; HUMAN HEALTH ; IN-VIVO ; LIVER ; TOXICITY ; EXPOSURE
Funding OrganizationNational Natural Science Foundations of China ; National Natural Science Foundations of China ; (31070457) ; (31070457) ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; (31070457) ; (31070457)
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27356
Collection淡水生态学研究中心_期刊论文
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Li, Shangchun,Chen, Jun,Xie, Ping,et al. The Role of Glutathione Detoxification Pathway in MCLR-Induced Hepatotoxicity in SD Rats[J]. ENVIRONMENTAL TOXICOLOGY,2015,30(12):1470-1480.
APA Li, Shangchun.,Chen, Jun.,Xie, Ping.,Guo, Xiaochun.,Fan, Huihui.,...&Chen, Liang.(2015).The Role of Glutathione Detoxification Pathway in MCLR-Induced Hepatotoxicity in SD Rats.ENVIRONMENTAL TOXICOLOGY,30(12),1470-1480.
MLA Li, Shangchun,et al."The Role of Glutathione Detoxification Pathway in MCLR-Induced Hepatotoxicity in SD Rats".ENVIRONMENTAL TOXICOLOGY 30.12(2015):1470-1480.
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