| Specific Cleavage of the Nucleoprotein of Fish Rhabdovirus | |
| Zhou, G. -Z.1; Yi, Y. -J.1; Chen, Z. -Y.2; Zhang, Q. -Y.2 | |
| 2015-11-01 | |
| Source Publication | VETERINARY PATHOLOGY
 |
| ISSN | 0300-9858 |
| Volume | 52Issue:6Pages:1258-1262 |
| Abstract | Siniperca chuatsi rhabdovirus (SCRV) is one of myriad rhabdoviruses recorded in fish. Preliminary data show that inhibition of the SCRV nucleoprotein (N) could significantly reduce the progeny virus titers in infected Epithelioma papulosum cyprinid (EPC) cells. Here, the authors propose that cleavage of the viral 47-kDa N protein is caspase-mediated based on caspase inhibition experiments, transient expression in EPC transfection, and analysis of cleavage sites. Cleavage of the SCRV N protein in culture was prevented by a pan-caspase inhibitor, z-VAD-FMK (z-Val-Ala-DL-Asp-fluoromethyl ketone). Subsequently, N was transiently expressed in EPC cells, the results of which indicated that the specific cleavage of N also occurred in the cells transfected with N-GFP plasmid. Several truncated fragments of the N gene were constructed and transiently transfected into EPC cells. Immunoblotting results indicated that D324 and D374 are the cleavage sites of N by caspases. The authors also found that z-VAD-FMK could inhibit the cytopathic effect in SCRV-infected EPC cells but not affect the production of infectious progeny, suggesting that the caspase-mediated cleavage of N protein is not required for in vitro SCRV replication. To the authors' knowledge, this is the first report on the cleavage of rhabdovirus proteins. |
| Subtype | Article |
| Keyword | Mandarin Fish Rhabdovirus Nucleoprotein Cleavage Caspases Virology Apoptosis |
| DOI | 10.1177/0300985815570068 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Natural Science Foundation of China(31101931) ; National Natural Science Foundation of China(31101931) ; Plan for Scientific Innovation Talent of Henan University of Technology(11CXRC13) ; Plan for Scientific Innovation Talent of Henan University of Technology(11CXRC13) ; Training Program for Youth Backbone Teacher of Henan University of Technology ; Training Program for Youth Backbone Teacher of Henan University of Technology ; Earmarked Fund for Conventional Freshwater Fish Industries of Henan Province(S2014-10-G01) ; Earmarked Fund for Conventional Freshwater Fish Industries of Henan Province(S2014-10-G01) ; National Natural Science Foundation of China(31101931) ; National Natural Science Foundation of China(31101931) ; Plan for Scientific Innovation Talent of Henan University of Technology(11CXRC13) ; Plan for Scientific Innovation Talent of Henan University of Technology(11CXRC13) ; Training Program for Youth Backbone Teacher of Henan University of Technology ; Training Program for Youth Backbone Teacher of Henan University of Technology ; Earmarked Fund for Conventional Freshwater Fish Industries of Henan Province(S2014-10-G01) ; Earmarked Fund for Conventional Freshwater Fish Industries of Henan Province(S2014-10-G01) |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Pathology ; Veterinary Sciences |
| WOS Subject | Pathology ; Veterinary Sciences |
| WOS ID | WOS:000364734300039 |
| WOS Keyword | VIRUS-INFECTION ; CELL-LINE ; APOPTOSIS |
| Funding Organization | National Natural Science Foundation of China(31101931) ; National Natural Science Foundation of China(31101931) ; Plan for Scientific Innovation Talent of Henan University of Technology(11CXRC13) ; Plan for Scientific Innovation Talent of Henan University of Technology(11CXRC13) ; Training Program for Youth Backbone Teacher of Henan University of Technology ; Training Program for Youth Backbone Teacher of Henan University of Technology ; Earmarked Fund for Conventional Freshwater Fish Industries of Henan Province(S2014-10-G01) ; Earmarked Fund for Conventional Freshwater Fish Industries of Henan Province(S2014-10-G01) ; National Natural Science Foundation of China(31101931) ; National Natural Science Foundation of China(31101931) ; Plan for Scientific Innovation Talent of Henan University of Technology(11CXRC13) ; Plan for Scientific Innovation Talent of Henan University of Technology(11CXRC13) ; Training Program for Youth Backbone Teacher of Henan University of Technology ; Training Program for Youth Backbone Teacher of Henan University of Technology ; Earmarked Fund for Conventional Freshwater Fish Industries of Henan Province(S2014-10-G01) ; Earmarked Fund for Conventional Freshwater Fish Industries of Henan Province(S2014-10-G01) |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/27354 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Affiliation | 1.Henan Univ Technol, Coll Bioengn, Zhengzhou 450001, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China |
| Recommended Citation GB/T 7714 | Zhou, G. -Z.,Yi, Y. -J.,Chen, Z. -Y.,et al. Specific Cleavage of the Nucleoprotein of Fish Rhabdovirus[J]. VETERINARY PATHOLOGY,2015,52(6):1258-1262. |
| APA | Zhou, G. -Z.,Yi, Y. -J.,Chen, Z. -Y.,&Zhang, Q. -Y..(2015).Specific Cleavage of the Nucleoprotein of Fish Rhabdovirus.VETERINARY PATHOLOGY,52(6),1258-1262. |
| MLA | Zhou, G. -Z.,et al."Specific Cleavage of the Nucleoprotein of Fish Rhabdovirus".VETERINARY PATHOLOGY 52.6(2015):1258-1262. |
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