Science & Technology
; Life Sciences & Biomedicine
类目[WOS]:
Pathology
; Veterinary Sciences
研究领域[WOS]:
Pathology
; Veterinary Sciences
英文摘要:
Siniperca chuatsi rhabdovirus (SCRV) is one of myriad rhabdoviruses recorded in fish. Preliminary data show that inhibition of the SCRV nucleoprotein (N) could significantly reduce the progeny virus titers in infected Epithelioma papulosum cyprinid (EPC) cells. Here, the authors propose that cleavage of the viral 47-kDa N protein is caspase-mediated based on caspase inhibition experiments, transient expression in EPC transfection, and analysis of cleavage sites. Cleavage of the SCRV N protein in culture was prevented by a pan-caspase inhibitor, z-VAD-FMK (z-Val-Ala-DL-Asp-fluoromethyl ketone). Subsequently, N was transiently expressed in EPC cells, the results of which indicated that the specific cleavage of N also occurred in the cells transfected with N-GFP plasmid. Several truncated fragments of the N gene were constructed and transiently transfected into EPC cells. Immunoblotting results indicated that D324 and D374 are the cleavage sites of N by caspases. The authors also found that z-VAD-FMK could inhibit the cytopathic effect in SCRV-infected EPC cells but not affect the production of infectious progeny, suggesting that the caspase-mediated cleavage of N protein is not required for in vitro SCRV replication. To the authors' knowledge, this is the first report on the cleavage of rhabdovirus proteins.
关键词[WOS]:
VIRUS-INFECTION
; CELL-LINE
; APOPTOSIS
语种:
英语
项目资助者:
National Natural Science Foundation of China(31101931)
; Plan for Scientific Innovation Talent of Henan University of Technology(11CXRC13)
; Training Program for Youth Backbone Teacher of Henan University of Technology
; Earmarked Fund for Conventional Freshwater Fish Industries of Henan Province(S2014-10-G01)
