IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
Specific Cleavage of the Nucleoprotein of Fish Rhabdovirus
Zhou, G. -Z.1; Yi, Y. -J.1; Chen, Z. -Y.2; Zhang, Q. -Y.2
2015-11-01
Source PublicationVETERINARY PATHOLOGY
ISSN0300-9858
Volume52Issue:6Pages:1258-1262
AbstractSiniperca chuatsi rhabdovirus (SCRV) is one of myriad rhabdoviruses recorded in fish. Preliminary data show that inhibition of the SCRV nucleoprotein (N) could significantly reduce the progeny virus titers in infected Epithelioma papulosum cyprinid (EPC) cells. Here, the authors propose that cleavage of the viral 47-kDa N protein is caspase-mediated based on caspase inhibition experiments, transient expression in EPC transfection, and analysis of cleavage sites. Cleavage of the SCRV N protein in culture was prevented by a pan-caspase inhibitor, z-VAD-FMK (z-Val-Ala-DL-Asp-fluoromethyl ketone). Subsequently, N was transiently expressed in EPC cells, the results of which indicated that the specific cleavage of N also occurred in the cells transfected with N-GFP plasmid. Several truncated fragments of the N gene were constructed and transiently transfected into EPC cells. Immunoblotting results indicated that D324 and D374 are the cleavage sites of N by caspases. The authors also found that z-VAD-FMK could inhibit the cytopathic effect in SCRV-infected EPC cells but not affect the production of infectious progeny, suggesting that the caspase-mediated cleavage of N protein is not required for in vitro SCRV replication. To the authors' knowledge, this is the first report on the cleavage of rhabdovirus proteins.
SubtypeArticle
KeywordMandarin Fish Rhabdovirus Nucleoprotein Cleavage Caspases Virology Apoptosis
DOI10.1177/0300985815570068
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31101931) ; National Natural Science Foundation of China(31101931) ; Plan for Scientific Innovation Talent of Henan University of Technology(11CXRC13) ; Plan for Scientific Innovation Talent of Henan University of Technology(11CXRC13) ; Training Program for Youth Backbone Teacher of Henan University of Technology ; Training Program for Youth Backbone Teacher of Henan University of Technology ; Earmarked Fund for Conventional Freshwater Fish Industries of Henan Province(S2014-10-G01) ; Earmarked Fund for Conventional Freshwater Fish Industries of Henan Province(S2014-10-G01) ; National Natural Science Foundation of China(31101931) ; National Natural Science Foundation of China(31101931) ; Plan for Scientific Innovation Talent of Henan University of Technology(11CXRC13) ; Plan for Scientific Innovation Talent of Henan University of Technology(11CXRC13) ; Training Program for Youth Backbone Teacher of Henan University of Technology ; Training Program for Youth Backbone Teacher of Henan University of Technology ; Earmarked Fund for Conventional Freshwater Fish Industries of Henan Province(S2014-10-G01) ; Earmarked Fund for Conventional Freshwater Fish Industries of Henan Province(S2014-10-G01)
Language英语
WOS Research AreaPathology ; Veterinary Sciences
WOS SubjectPathology ; Veterinary Sciences
WOS KeywordVIRUS-INFECTION ; CELL-LINE ; APOPTOSIS
WOS IDWOS:000364734300039
Funding OrganizationNational Natural Science Foundation of China(31101931) ; National Natural Science Foundation of China(31101931) ; Plan for Scientific Innovation Talent of Henan University of Technology(11CXRC13) ; Plan for Scientific Innovation Talent of Henan University of Technology(11CXRC13) ; Training Program for Youth Backbone Teacher of Henan University of Technology ; Training Program for Youth Backbone Teacher of Henan University of Technology ; Earmarked Fund for Conventional Freshwater Fish Industries of Henan Province(S2014-10-G01) ; Earmarked Fund for Conventional Freshwater Fish Industries of Henan Province(S2014-10-G01) ; National Natural Science Foundation of China(31101931) ; National Natural Science Foundation of China(31101931) ; Plan for Scientific Innovation Talent of Henan University of Technology(11CXRC13) ; Plan for Scientific Innovation Talent of Henan University of Technology(11CXRC13) ; Training Program for Youth Backbone Teacher of Henan University of Technology ; Training Program for Youth Backbone Teacher of Henan University of Technology ; Earmarked Fund for Conventional Freshwater Fish Industries of Henan Province(S2014-10-G01) ; Earmarked Fund for Conventional Freshwater Fish Industries of Henan Province(S2014-10-G01)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27354
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Henan Univ Technol, Coll Bioengn, Zhengzhou 450001, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Zhou, G. -Z.,Yi, Y. -J.,Chen, Z. -Y.,et al. Specific Cleavage of the Nucleoprotein of Fish Rhabdovirus[J]. VETERINARY PATHOLOGY,2015,52(6):1258-1262.
APA Zhou, G. -Z.,Yi, Y. -J.,Chen, Z. -Y.,&Zhang, Q. -Y..(2015).Specific Cleavage of the Nucleoprotein of Fish Rhabdovirus.VETERINARY PATHOLOGY,52(6),1258-1262.
MLA Zhou, G. -Z.,et al."Specific Cleavage of the Nucleoprotein of Fish Rhabdovirus".VETERINARY PATHOLOGY 52.6(2015):1258-1262.
Files in This Item:
File Name/Size DocType Version Access License
Specific Cleavage of(865KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhou, G. -Z.]'s Articles
[Yi, Y. -J.]'s Articles
[Chen, Z. -Y.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhou, G. -Z.]'s Articles
[Yi, Y. -J.]'s Articles
[Chen, Z. -Y.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhou, G. -Z.]'s Articles
[Yi, Y. -J.]'s Articles
[Chen, Z. -Y.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Specific Cleavage of the Nucleoprotein of Fish Rhabdovirus.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.