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题名: Molecular characterization and expression of an oocyte-specific histone stem-loop binding protein in Carassius gibelio
作者: Liu, Zhen1; Zhang, Xiao-Juan1; Wang, Wei1; Zhang, Jun1; Li, Zhi1; Gui, Jian-Fang1
关键词: Carassius gibelio ; Stem-loop-binding protein ; SLBP2 ; Oocyte ; Oogenesis
刊名: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
发表日期: 2015-12-01
DOI: 10.1016/j.cbpb.2015.08.011
卷: 190, 页:46-53
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Zoology
研究领域[WOS]: Biochemistry & Molecular Biology ; Zoology
英文摘要: Stem-loop-binding proteins (SLBPs) have been revealed to interact with stem-loop of histones, and oocyte-specific and oocyte-preferential SLBP2 have been identified in vertebrates including Xenopus (S. tropicalis) and B. taurus to play a key role in histone translation regulation, but no oocyte-specific SLBPs have been characterized in fish. Here, we have identified and characterized the first fish oocyte-specific SLBP2 in Carassius gibelio. Its full-length cDNA contains 975 bp ORF encoding 324 amino acids. Firstly, the polyclonal antibody specific to C gibelio SLBP2 was prepared. Then, RT-PCR analysis and Western blot detection revealed its oocyte-specific and dynamic expression pattern during oogenesis and embryogenesis of C gibelio. Moreover, in situ hybridization and immunofluorescence localization observed its abundant expression in cortical alveolar stage oocytes and dynamic distribution in different stage oocytes. Altogether, our current data suggest that C. gibelio SLBP2 might play significant role in the early oogenesis and oocyte growth. (C) 2015 Elsevier Inc All rights reserved.
关键词[WOS]: SILVER CRUCIAN CARP ; PRE-MESSENGER-RNA ; AURATUS-GIBELIO ; CORTICAL GRANULES ; GENETIC-BASIS ; ZEBRAFISH ; FISH ; TRANSITION ; MATURATION ; OOGENESIS
语种: 英语
项目资助者: Modern Agro-industry Technology Research System(NYCYTX-49) ; National Natural Science Foundation(31123001) ; National Science and Technology Support Program(2012BA026B02) ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ22) ; Autonomous Projects of the Institute of Hydrobiology, CAS(Y25A17 ; Y45A171301)
WOS记录号: WOS:000364252600006
ISSN号: 1096-4959
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27347
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
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