IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
Molecular characterization and expression of an oocyte-specific histone stem-loop binding protein in Carassius gibelio
Liu, Zhen; Zhang, Xiao-Juan; Wang, Wei; Zhang, Jun; Li, Zhi; Gui, Jian-Fang
2015-12-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
Volume190Pages:46-53
AbstractStem-loop-binding proteins (SLBPs) have been revealed to interact with stem-loop of histones, and oocyte-specific and oocyte-preferential SLBP2 have been identified in vertebrates including Xenopus (S. tropicalis) and B. taurus to play a key role in histone translation regulation, but no oocyte-specific SLBPs have been characterized in fish. Here, we have identified and characterized the first fish oocyte-specific SLBP2 in Carassius gibelio. Its full-length cDNA contains 975 bp ORF encoding 324 amino acids. Firstly, the polyclonal antibody specific to C gibelio SLBP2 was prepared. Then, RT-PCR analysis and Western blot detection revealed its oocyte-specific and dynamic expression pattern during oogenesis and embryogenesis of C gibelio. Moreover, in situ hybridization and immunofluorescence localization observed its abundant expression in cortical alveolar stage oocytes and dynamic distribution in different stage oocytes. Altogether, our current data suggest that C. gibelio SLBP2 might play significant role in the early oogenesis and oocyte growth. (C) 2015 Elsevier Inc All rights reserved.
SubtypeArticle
KeywordCarassius Gibelio Stem-loop-binding Protein Slbp2 Oocyte Oogenesis
DOI10.1016/j.cbpb.2015.08.011
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationModern Agro-industry Technology Research System(NYCYTX-49) ; Modern Agro-industry Technology Research System(NYCYTX-49) ; National Natural Science Foundation(31123001) ; National Natural Science Foundation(31123001) ; National Science and Technology Support Program(2012BA026B02) ; National Science and Technology Support Program(2012BA026B02) ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ22) ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ22) ; Autonomous Projects of the Institute of Hydrobiology, CAS(Y25A17 ; Autonomous Projects of the Institute of Hydrobiology, CAS(Y25A17 ; Y45A171301) ; Y45A171301) ; Modern Agro-industry Technology Research System(NYCYTX-49) ; Modern Agro-industry Technology Research System(NYCYTX-49) ; National Natural Science Foundation(31123001) ; National Natural Science Foundation(31123001) ; National Science and Technology Support Program(2012BA026B02) ; National Science and Technology Support Program(2012BA026B02) ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ22) ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ22) ; Autonomous Projects of the Institute of Hydrobiology, CAS(Y25A17 ; Autonomous Projects of the Institute of Hydrobiology, CAS(Y25A17 ; Y45A171301) ; Y45A171301)
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Zoology
WOS SubjectBiochemistry & Molecular Biology ; Zoology
WOS KeywordSILVER CRUCIAN CARP ; PRE-MESSENGER-RNA ; AURATUS-GIBELIO ; CORTICAL GRANULES ; GENETIC-BASIS ; ZEBRAFISH ; FISH ; TRANSITION ; MATURATION ; OOGENESIS
WOS IDWOS:000364252600006
Funding OrganizationModern Agro-industry Technology Research System(NYCYTX-49) ; Modern Agro-industry Technology Research System(NYCYTX-49) ; National Natural Science Foundation(31123001) ; National Natural Science Foundation(31123001) ; National Science and Technology Support Program(2012BA026B02) ; National Science and Technology Support Program(2012BA026B02) ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ22) ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ22) ; Autonomous Projects of the Institute of Hydrobiology, CAS(Y25A17 ; Autonomous Projects of the Institute of Hydrobiology, CAS(Y25A17 ; Y45A171301) ; Y45A171301) ; Modern Agro-industry Technology Research System(NYCYTX-49) ; Modern Agro-industry Technology Research System(NYCYTX-49) ; National Natural Science Foundation(31123001) ; National Natural Science Foundation(31123001) ; National Science and Technology Support Program(2012BA026B02) ; National Science and Technology Support Program(2012BA026B02) ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ22) ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ22) ; Autonomous Projects of the Institute of Hydrobiology, CAS(Y25A17 ; Autonomous Projects of the Institute of Hydrobiology, CAS(Y25A17 ; Y45A171301) ; Y45A171301)
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27347
Collection鱼类生物学及渔业生物技术研究中心
AffiliationUniv Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Liu, Zhen,Zhang, Xiao-Juan,Wang, Wei,et al. Molecular characterization and expression of an oocyte-specific histone stem-loop binding protein in Carassius gibelio[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2015,190:46-53.
APA Liu, Zhen,Zhang, Xiao-Juan,Wang, Wei,Zhang, Jun,Li, Zhi,&Gui, Jian-Fang.(2015).Molecular characterization and expression of an oocyte-specific histone stem-loop binding protein in Carassius gibelio.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,190,46-53.
MLA Liu, Zhen,et al."Molecular characterization and expression of an oocyte-specific histone stem-loop binding protein in Carassius gibelio".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 190(2015):46-53.
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