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题名: ELL Protein-associated Factor 2 (EAF2) Inhibits Transforming Growth Factor beta Signaling through a Direct Interaction with Smad3
作者: Liu, Xing1; Chen, Zhu1, 2; Ouyang, Gang1; Song, Tieshan3; Liang, Huageng4; Liu, Wei1; Xiao, Wuhan1
刊名: JOURNAL OF BIOLOGICAL CHEMISTRY
发表日期: 2015-10-23
DOI: 10.1074/jbc.M115.663542
卷: 290, 期:43, 页:25933-25945
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: A series of in vitro and in vivo studies has shown that EAF2 can affect multiple signaling pathways involved in cellular processes. However, the molecular mechanisms underlying its effects have remained elusive. Here we report the discovery of a new functional link between EAF2 and TGF-beta signaling. Promoter reporter assays indicated that EAF2 suppresses Smad3 transcriptional activity, resulting in inhibition of TGF-beta signaling. Coimmunoprecipitation assays showed that EAF2 specifically interacts with Smad3 in vitro and in vivo but not with other Smad proteins. In addition, we observed that EAF2 binding does not alter Smad3 phosphorylation but causes Smad3 cytoplasmic retention, competes with Smad4 for binding to Smad3, and prevents p300-Smad3 complex formation. Furthermore, we demonstrated that EAF2 suppresses both TGF-beta -induced G(1) cell cycle arrest and TGF-beta -induced cell migration. This study identifies and characterizes a novel repressor of TGF-beta signaling.
关键词[WOS]: TGF-BETA ; NUCLEAR EXPORT ; TRANSCRIPTIONAL ACTIVITY ; II RECEPTORS ; COACTIVATORS ; EXPRESSION ; CBP/P300 ; TRANSLOCATION ; TRANSDUCTION ; CONVERGENCE
语种: 英语
项目资助者: CAS Major Scientific and Technological Project(XDA08010208) ; NSFC (National Natural Science Foundation of China)(31461163003 ; 31071212 ; 91019008 ; 31401072 ; 81160266/H1603)
WOS记录号: WOS:000363527300016
ISSN号: 0021-9258
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27344
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
2.Maternal & Child Hlth Hosp Hubei Prov, Dept Reprod, Wuhan 430070, Peoples R China
3.Hubei Univ Sci & Technol, Xianning 437100, Peoples R China
4.Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Urol, Wuhan 430030, Peoples R China
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