| ELL Protein-associated Factor 2 (EAF2) Inhibits Transforming Growth Factor beta Signaling through a Direct Interaction with Smad3 | |
| Liu, Xing1; Chen, Zhu1,2; Ouyang, Gang1; Song, Tieshan3; Liang, Huageng4; Liu, Wei1; Xiao, Wuhan1 | |
| 2015-10-23 | |
| Source Publication | JOURNAL OF BIOLOGICAL CHEMISTRY
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| ISSN | 0021-9258 |
| Volume | 290Issue:43Pages:25933-25945 |
| Abstract | A series of in vitro and in vivo studies has shown that EAF2 can affect multiple signaling pathways involved in cellular processes. However, the molecular mechanisms underlying its effects have remained elusive. Here we report the discovery of a new functional link between EAF2 and TGF-beta signaling. Promoter reporter assays indicated that EAF2 suppresses Smad3 transcriptional activity, resulting in inhibition of TGF-beta signaling. Coimmunoprecipitation assays showed that EAF2 specifically interacts with Smad3 in vitro and in vivo but not with other Smad proteins. In addition, we observed that EAF2 binding does not alter Smad3 phosphorylation but causes Smad3 cytoplasmic retention, competes with Smad4 for binding to Smad3, and prevents p300-Smad3 complex formation. Furthermore, we demonstrated that EAF2 suppresses both TGF-beta -induced G(1) cell cycle arrest and TGF-beta -induced cell migration. This study identifies and characterizes a novel repressor of TGF-beta signaling. |
| Subtype | Article |
| DOI | 10.1074/jbc.M115.663542 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | CAS Major Scientific and Technological Project(XDA08010208) ; CAS Major Scientific and Technological Project(XDA08010208) ; NSFC (National Natural Science Foundation of China)(31461163003 ; NSFC (National Natural Science Foundation of China)(31461163003 ; 31071212 ; 31071212 ; 91019008 ; 91019008 ; 31401072 ; 31401072 ; 81160266/H1603) ; 81160266/H1603) ; CAS Major Scientific and Technological Project(XDA08010208) ; CAS Major Scientific and Technological Project(XDA08010208) ; NSFC (National Natural Science Foundation of China)(31461163003 ; NSFC (National Natural Science Foundation of China)(31461163003 ; 31071212 ; 31071212 ; 91019008 ; 91019008 ; 31401072 ; 31401072 ; 81160266/H1603) ; 81160266/H1603) |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology |
| WOS Subject | Biochemistry & Molecular Biology |
| WOS ID | WOS:000363527300016 |
| WOS Keyword | TGF-BETA ; NUCLEAR EXPORT ; TRANSCRIPTIONAL ACTIVITY ; II RECEPTORS ; COACTIVATORS ; EXPRESSION ; CBP/P300 ; TRANSLOCATION ; TRANSDUCTION ; CONVERGENCE |
| Funding Organization | CAS Major Scientific and Technological Project(XDA08010208) ; CAS Major Scientific and Technological Project(XDA08010208) ; NSFC (National Natural Science Foundation of China)(31461163003 ; NSFC (National Natural Science Foundation of China)(31461163003 ; 31071212 ; 31071212 ; 91019008 ; 91019008 ; 31401072 ; 31401072 ; 81160266/H1603) ; 81160266/H1603) ; CAS Major Scientific and Technological Project(XDA08010208) ; CAS Major Scientific and Technological Project(XDA08010208) ; NSFC (National Natural Science Foundation of China)(31461163003 ; NSFC (National Natural Science Foundation of China)(31461163003 ; 31071212 ; 31071212 ; 91019008 ; 91019008 ; 31401072 ; 31401072 ; 81160266/H1603) ; 81160266/H1603) |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/27344 |
| Collection | 水生生物分子与细胞生物学研究中心_期刊论文 |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China 2.Maternal & Child Hlth Hosp Hubei Prov, Dept Reprod, Wuhan 430070, Peoples R China 3.Hubei Univ Sci & Technol, Xianning 437100, Peoples R China 4.Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Urol, Wuhan 430030, Peoples R China |
| Recommended Citation GB/T 7714 | Liu, Xing,Chen, Zhu,Ouyang, Gang,et al. ELL Protein-associated Factor 2 (EAF2) Inhibits Transforming Growth Factor beta Signaling through a Direct Interaction with Smad3[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2015,290(43):25933-25945. |
| APA | Liu, Xing.,Chen, Zhu.,Ouyang, Gang.,Song, Tieshan.,Liang, Huageng.,...&Xiao, Wuhan.(2015).ELL Protein-associated Factor 2 (EAF2) Inhibits Transforming Growth Factor beta Signaling through a Direct Interaction with Smad3.JOURNAL OF BIOLOGICAL CHEMISTRY,290(43),25933-25945. |
| MLA | Liu, Xing,et al."ELL Protein-associated Factor 2 (EAF2) Inhibits Transforming Growth Factor beta Signaling through a Direct Interaction with Smad3".JOURNAL OF BIOLOGICAL CHEMISTRY 290.43(2015):25933-25945. |
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