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Interferon regulatory factor 10 (IRF10): Cloning in orange spotted grouper, Epinephelus coioides, and evolutionary analysis in vertebrates
Huang, Bei1; Jia, Qin Qin1; Liang, Ying1; Huang, Wen Shu1; Nie, P.1,2
2015-10-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume46Issue:2Pages:669-677
AbstractIRF10 gene was cloned in orange spotted grouper, Epinephelus coioides, and its expression was examined following poly(I:C) stimulation and bacterial infection. The cDNA sequence of grouper IRF10 contains an open reading frame of 1197 bp, flanked by 99 bp 5'-untranslated region and 480 bp 3'- untranslated region. Multiple alignments showed that the grouper IRF10 has a highly conserved DNA binding domain in the N terminus with characteristic motif containing five tryptophan residues. Quantitative real-time PCR analysis revealed that the expression of IRF10 was responsive to both poly(I:C) stimulation and Vibrio parahemolyticus infection, with a higher increase to poly(I:C), indicating an important role of IRF10 in host immune response during infection. A phyletic distribution of IRF members was also examined in vertebrates, and IRF10 was found in most lineages of vertebrates, not in modern primates and rodents. It is suggested that the first divergence of IRF members might have occurred before the evolutionary split of vertebrate and cephalochordates, producing ancestors of IRF (1/2/11) and IRF (4/8/9/10)[(3/7) (5/6)], and that the second and/or third divergence of IRF members occurred following the split, thus leading to the subsets of the IRF family in vertebrates. (C) 2015 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordIrf10 Orange Spotted Grouper Epinephelus Coioides Evolution Vertebrate
DOI10.1016/j.fsi.2015.08.003
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31402329 ; National Natural Science Foundation of China(31402329 ; Natural Science Foundation of Fujian province(2012J06008) ; Natural Science Foundation of Fujian province(2012J06008) ; National 863 Program of China(2012AA092201) ; National 863 Program of China(2012AA092201) ; Science and Technology Program of Xiamen Southern Oceanographic Center(14PYY050SF03) ; Science and Technology Program of Xiamen Southern Oceanographic Center(14PYY050SF03) ; 31172438) ; 31172438) ; National Natural Science Foundation of China(31402329 ; National Natural Science Foundation of China(31402329 ; Natural Science Foundation of Fujian province(2012J06008) ; Natural Science Foundation of Fujian province(2012J06008) ; National 863 Program of China(2012AA092201) ; National 863 Program of China(2012AA092201) ; Science and Technology Program of Xiamen Southern Oceanographic Center(14PYY050SF03) ; Science and Technology Program of Xiamen Southern Oceanographic Center(14PYY050SF03) ; 31172438) ; 31172438)
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordPROTEIN-PROTEIN INTERACTIONS ; COD GADUS-MORHUA ; TRANSCRIPTION FACTORS ; ONCORHYNCHUS-MYKISS ; MOLECULAR-CLONING ; CRYSTAL-STRUCTURE ; RAINBOW-TROUT ; DNA-SEQUENCES ; HOST-DEFENSE ; FAMILY
WOS IDWOS:000363071200056
Funding OrganizationNational Natural Science Foundation of China(31402329 ; National Natural Science Foundation of China(31402329 ; Natural Science Foundation of Fujian province(2012J06008) ; Natural Science Foundation of Fujian province(2012J06008) ; National 863 Program of China(2012AA092201) ; National 863 Program of China(2012AA092201) ; Science and Technology Program of Xiamen Southern Oceanographic Center(14PYY050SF03) ; Science and Technology Program of Xiamen Southern Oceanographic Center(14PYY050SF03) ; 31172438) ; 31172438) ; National Natural Science Foundation of China(31402329 ; National Natural Science Foundation of China(31402329 ; Natural Science Foundation of Fujian province(2012J06008) ; Natural Science Foundation of Fujian province(2012J06008) ; National 863 Program of China(2012AA092201) ; National 863 Program of China(2012AA092201) ; Science and Technology Program of Xiamen Southern Oceanographic Center(14PYY050SF03) ; Science and Technology Program of Xiamen Southern Oceanographic Center(14PYY050SF03) ; 31172438) ; 31172438)
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27338
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China
2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Huang, Bei,Jia, Qin Qin,Liang, Ying,et al. Interferon regulatory factor 10 (IRF10): Cloning in orange spotted grouper, Epinephelus coioides, and evolutionary analysis in vertebrates[J]. FISH & SHELLFISH IMMUNOLOGY,2015,46(2):669-677.
APA Huang, Bei,Jia, Qin Qin,Liang, Ying,Huang, Wen Shu,&Nie, P..(2015).Interferon regulatory factor 10 (IRF10): Cloning in orange spotted grouper, Epinephelus coioides, and evolutionary analysis in vertebrates.FISH & SHELLFISH IMMUNOLOGY,46(2),669-677.
MLA Huang, Bei,et al."Interferon regulatory factor 10 (IRF10): Cloning in orange spotted grouper, Epinephelus coioides, and evolutionary analysis in vertebrates".FISH & SHELLFISH IMMUNOLOGY 46.2(2015):669-677.
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