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题名: Investigating the potential immune role of fish NCAMs: Molecular cloning and expression analysis in mandarin fish
作者: Wu, Nan1; Zhang, Xiang-Yang1, 2; Huang, Bei3; Zhang, Nu1, 2; Zhang, Xu-Jie1, 4; Guo, Xia1, 5; Chen, Xiao-Ling1; Zhang, Yu1; Wu, Han1; Li, Shun1; Li, Ai-Hua1; Zhang, Yong-An1
关键词: NCAM ; NCCRP-1 ; NK cell ; Systemic immune ; Mucosal immune ; Mandarin fish
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2015-10-01
DOI: 10.1016/j.fsi.2015.08.006
卷: 46, 期:2, 页:765-777
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
英文摘要: The immune role of NCAMs has been revealed in mammals, yet there is no such report in fish. Hence, we analyzed the molecular characterizations and immune-associated expression patterns of NCAMs in mandarin fish. Three NCAM members, named mfNCAM1a, mfNCAM1b and mfNCAM2, were identified. Among the cDNA sequences of mJNCAMs, AU-rich elements in the 3' UTRs of mfNCAM1b and mfNCAM2 as well as VASE sequences in the fourth Ig-like domain-encoding regions of mfNCAM1a and mfNCAM1b were discovered. Moreover, the syntenic analysis suggested that the duplication of NCAM1 is fish-specific. At mRNA and protein levels, the expression analyses revealed that mfNCAMs existed in both systemic and mucosal immune tissues, and located within lymphoid cells. Upon stimulated either by LPS or poly I:C, the expression level of mfNCAM1a was significantly up-regulated in head kidney, spleen, liver, and gut, whereas mfNCAM1b only in head kidney and liver, and mfNCAM2 only in liver. Additionally, the cells coexpressed mfNCAM1 and mfNCCRP-1 might imply the equivalents to mammalian NK cells. Our finding firstly demonstrates the member-specific immune-related tissue expression pattern and immune activity for fish NCAMs. Current data indicate that mfNCAM2 has little immune activity, while the immune activity of mfNCAM1a exists in more tissues than mfNCAM1b, and mfNCAM1a may tend to respond more actively to viral while mfNCAM1b to bacterial stimulants. Additionally, NCAM1b should be a fish-specific member with unique immune function, judging from its different expression pattern, immune activity as well as phylogenetic relationship to mfNCAM1a. (C) 2015 Elsevier Ltd. All rights reserved.
关键词[WOS]: CELL-ADHESION MOLECULE ; NATURAL-KILLER-CELLS ; IN-SITU HYBRIDIZATION ; CYTOTOXIC-CELLS ; OREOCHROMIS-NILOTICUS ; RECEPTOR (NCCRP)-1 ; POLYSIALIC ACID ; STEM-CELLS ; NK CELLS ; T-CELLS
语种: 英语
项目资助者: National Natural Science Foundation of China(31172431 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2012FB20) ; 31472307)
WOS记录号: WOS:000363071200067
ISSN号: 1050-4648
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27337
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Jimei Univ, Coll Fisheries, Xiamen 361021, Peoples R China
4.Shanghai Ocean Univ, Shanghai 201306, Peoples R China
5.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 4302231, Peoples R China
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