Investigating the potential immune role of fish NCAMs: Molecular cloning and expression analysis in mandarin fish
Wu, Nan1; Zhang, Xiang-Yang1,2; Huang, Bei3; Zhang, Nu1,2; Zhang, Xu-Jie1,4; Guo, Xia1,5; Chen, Xiao-Ling1; Zhang, Yu1; Wu, Han1; Li, Shun1; Li, Ai-Hua1; Zhang, Yong-An1
2015-10-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume46Issue:2Pages:765-777
AbstractThe immune role of NCAMs has been revealed in mammals, yet there is no such report in fish. Hence, we analyzed the molecular characterizations and immune-associated expression patterns of NCAMs in mandarin fish. Three NCAM members, named mfNCAM1a, mfNCAM1b and mfNCAM2, were identified. Among the cDNA sequences of mJNCAMs, AU-rich elements in the 3' UTRs of mfNCAM1b and mfNCAM2 as well as VASE sequences in the fourth Ig-like domain-encoding regions of mfNCAM1a and mfNCAM1b were discovered. Moreover, the syntenic analysis suggested that the duplication of NCAM1 is fish-specific. At mRNA and protein levels, the expression analyses revealed that mfNCAMs existed in both systemic and mucosal immune tissues, and located within lymphoid cells. Upon stimulated either by LPS or poly I:C, the expression level of mfNCAM1a was significantly up-regulated in head kidney, spleen, liver, and gut, whereas mfNCAM1b only in head kidney and liver, and mfNCAM2 only in liver. Additionally, the cells coexpressed mfNCAM1 and mfNCCRP-1 might imply the equivalents to mammalian NK cells. Our finding firstly demonstrates the member-specific immune-related tissue expression pattern and immune activity for fish NCAMs. Current data indicate that mfNCAM2 has little immune activity, while the immune activity of mfNCAM1a exists in more tissues than mfNCAM1b, and mfNCAM1a may tend to respond more actively to viral while mfNCAM1b to bacterial stimulants. Additionally, NCAM1b should be a fish-specific member with unique immune function, judging from its different expression pattern, immune activity as well as phylogenetic relationship to mfNCAM1a. (C) 2015 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordNcam Nccrp-1 Nk Cell Systemic Immune Mucosal Immune Mandarin Fish
DOI10.1016/j.fsi.2015.08.006
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31172431 ; National Natural Science Foundation of China(31172431 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2012FB20) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2012FB20) ; 31472307) ; 31472307) ; National Natural Science Foundation of China(31172431 ; National Natural Science Foundation of China(31172431 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2012FB20) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2012FB20) ; 31472307) ; 31472307)
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordCELL-ADHESION MOLECULE ; NATURAL-KILLER-CELLS ; IN-SITU HYBRIDIZATION ; CYTOTOXIC-CELLS ; OREOCHROMIS-NILOTICUS ; RECEPTOR (NCCRP)-1 ; POLYSIALIC ACID ; STEM-CELLS ; NK CELLS ; T-CELLS
WOS IDWOS:000363071200067
Funding OrganizationNational Natural Science Foundation of China(31172431 ; National Natural Science Foundation of China(31172431 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2012FB20) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2012FB20) ; 31472307) ; 31472307) ; National Natural Science Foundation of China(31172431 ; National Natural Science Foundation of China(31172431 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2012FB20) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2012FB20) ; 31472307) ; 31472307)
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27337
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Jimei Univ, Coll Fisheries, Xiamen 361021, Peoples R China
4.Shanghai Ocean Univ, Shanghai 201306, Peoples R China
5.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 4302231, Peoples R China
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Wu, Nan,Zhang, Xiang-Yang,Huang, Bei,et al. Investigating the potential immune role of fish NCAMs: Molecular cloning and expression analysis in mandarin fish[J]. FISH & SHELLFISH IMMUNOLOGY,2015,46(2):765-777.
APA Wu, Nan.,Zhang, Xiang-Yang.,Huang, Bei.,Zhang, Nu.,Zhang, Xu-Jie.,...&Zhang, Yong-An.(2015).Investigating the potential immune role of fish NCAMs: Molecular cloning and expression analysis in mandarin fish.FISH & SHELLFISH IMMUNOLOGY,46(2),765-777.
MLA Wu, Nan,et al."Investigating the potential immune role of fish NCAMs: Molecular cloning and expression analysis in mandarin fish".FISH & SHELLFISH IMMUNOLOGY 46.2(2015):765-777.
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