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题名: The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance
作者: Chen, Ji1; Luo, Qing1, 2; Bao, Hairong1; Liao, Lanjie1; Li, Yongming1; Zhu, Zuoyan1; Wang, Yaping1; Hu, Wei1
关键词: Common carp ; Growth hormone ; Transgene ; Integration site ; Copy number
刊名: SCIENCE BULLETIN
发表日期: 2015-10-01
DOI: 10.1007/s11434-015-0893-x
卷: 60, 期:19, 页:1654-1660
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: The genetic stability and expression efficiency of exogenous genes in transgenic animals are closely related to integration site and copy number. In our laboratory, by transgenic manipulation and subsequent test crosses, we established an "all-fish" growth hormone (GH) transgenic common carp family that exhibits fast growth. In this present study, genome walking, real-time quantitative polymerase chain reaction, and fluorescence in situ hybridization techniques were applied to identify the integration characteristics of the exogenous grass carp GH gene in the transgenic common carp. The exogenous GH genes, in the form of two complete and one incomplete tandem repeats, were found to have integrated into an AT-rich region near the end of a chromosome pair. We hypothesize that the high efficiency of exogenous GH gene expression might be due to the low copy number in the genome and the AT-rich integration site.
关键词[WOS]: SALMON ONCORHYNCHUS-KISUTCH ; MISGURNUS-MIZOLEPIS ; ACCELERATED-GROWTH ; ZEBRAFISH EMBRYOS ; ATLANTIC SALMON ; MUD LOACH ; FISH ; EXPRESSION ; GENOME ; CELLS
语种: 英语
项目资助者: National High Technology Research and Development Program of China ("863" Program)(2011AA100404 ; National Natural Science Foundation of China(31325026 ; 2011AA100403) ; 31272649)
WOS记录号: WOS:000363043800004
ISSN号: 2095-9273
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27332
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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