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The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance
Chen, Ji1; Luo, Qing1,2; Bao, Hairong1; Liao, Lanjie1; Li, Yongming1; Zhu, Zuoyan1; Wang, Yaping1; Hu, Wei1
2015-10-01
Source PublicationSCIENCE BULLETIN
ISSN2095-9273
Volume60Issue:19Pages:1654-1660
AbstractThe genetic stability and expression efficiency of exogenous genes in transgenic animals are closely related to integration site and copy number. In our laboratory, by transgenic manipulation and subsequent test crosses, we established an "all-fish" growth hormone (GH) transgenic common carp family that exhibits fast growth. In this present study, genome walking, real-time quantitative polymerase chain reaction, and fluorescence in situ hybridization techniques were applied to identify the integration characteristics of the exogenous grass carp GH gene in the transgenic common carp. The exogenous GH genes, in the form of two complete and one incomplete tandem repeats, were found to have integrated into an AT-rich region near the end of a chromosome pair. We hypothesize that the high efficiency of exogenous GH gene expression might be due to the low copy number in the genome and the AT-rich integration site.
SubtypeArticle
KeywordCommon Carp Growth Hormone Transgene Integration Site Copy Number
DOI10.1007/s11434-015-0893-x
WOS HeadingsScience & Technology
Indexed BySCI
Funding OrganizationNational High Technology Research and Development Program of China ("863" Program)(2011AA100404 ; National High Technology Research and Development Program of China ("863" Program)(2011AA100404 ; National Natural Science Foundation of China(31325026 ; National Natural Science Foundation of China(31325026 ; 2011AA100403) ; 2011AA100403) ; 31272649) ; 31272649) ; National High Technology Research and Development Program of China ("863" Program)(2011AA100404 ; National High Technology Research and Development Program of China ("863" Program)(2011AA100404 ; National Natural Science Foundation of China(31325026 ; National Natural Science Foundation of China(31325026 ; 2011AA100403) ; 2011AA100403) ; 31272649) ; 31272649)
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS KeywordSALMON ONCORHYNCHUS-KISUTCH ; MISGURNUS-MIZOLEPIS ; ACCELERATED-GROWTH ; ZEBRAFISH EMBRYOS ; ATLANTIC SALMON ; MUD LOACH ; FISH ; EXPRESSION ; GENOME ; CELLS
WOS IDWOS:000363043800004
Funding OrganizationNational High Technology Research and Development Program of China ("863" Program)(2011AA100404 ; National High Technology Research and Development Program of China ("863" Program)(2011AA100404 ; National Natural Science Foundation of China(31325026 ; National Natural Science Foundation of China(31325026 ; 2011AA100403) ; 2011AA100403) ; 31272649) ; 31272649) ; National High Technology Research and Development Program of China ("863" Program)(2011AA100404 ; National High Technology Research and Development Program of China ("863" Program)(2011AA100404 ; National Natural Science Foundation of China(31325026 ; National Natural Science Foundation of China(31325026 ; 2011AA100403) ; 2011AA100403) ; 31272649) ; 31272649)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27332
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Chen, Ji,Luo, Qing,Bao, Hairong,et al. The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance[J]. SCIENCE BULLETIN,2015,60(19):1654-1660.
APA Chen, Ji.,Luo, Qing.,Bao, Hairong.,Liao, Lanjie.,Li, Yongming.,...&Hu, Wei.(2015).The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance.SCIENCE BULLETIN,60(19),1654-1660.
MLA Chen, Ji,et al."The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance".SCIENCE BULLETIN 60.19(2015):1654-1660.
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