Manganese deficiency or excess caused the depression of intestinal immunity, induction of inflammation and dysfunction of the intestinal physical barrier, as regulated by NF-kappa B, TOR and Nrf2 signalling, in grass carp (Ctenopharyngodon idella)
Intestinal mucosal immune components and mRNA levels of inflammatory cytokines, tight junction proteins, antioxidant enzymes and related signalling molecules in young grass carp (Ctenopharyngodon idellus) under dietary manganese (Mn) deficiency or excess were investigated. Fish were fed the diets containing graded levels of Mn [3.65-27.86 mg Mn kg(-1) diet] for 8 weeks. The results demonstrated that Mn deficiency significantly decreased the lysozyme and acid phosphatase (ACP) activities, up-regulated tumour necrosis factor alpha (TNF-alpha), interleukin 8 and the signalling factor nuclear factor-kappa B p65, and down-regulated interleukin 10 (IL-10), transforming growth factor beta 1, inhibitor of signalling factors kappa B-alpha and target of rapamycin mRNA levels in the proximal intestine (PI), mid intestine (MI) and distal intestine (DI). However, Mn deficiency did not change the C3 content in the PI, whereas it decreased the C3 contents in the MI and DI. Additionally, Mn depletion also resulted in significantly low mRNA levels for tight junction proteins (claudin-b, claudin-c, claudin-15, occludin and zonula occludens-1), antioxidant enzymes (MnSOD, GPx and CAT) and NF-E2-related factor-2 in the intestines of fish. Excessive Mn exhibited toxic effects similar to Mn deficiency, where optimal Mn contents reversed those indicators. In conclusion, Mn deficiency or excess causes the depression of intestinal immunity, induction of inflammation and dysfunction of the intestinal physical barrier relating to NF-kappa B, TOR and Nrf2 signalling in grass carp. Furthermore, quadratic regression analysis at 95% maximum response of lysozyme and acid phosphatase activities in the distal intestine of young grass carp revealed the optimum dietary Mn levels to be 8.90 and 8.99 mg kg(-1) diet, respectively. (C) 2015 Elsevier Ltd. All rights reserved.
National Basic Research Program of China (973 Program)(2014CB138600)
; National Department Public Benefit Research Foundation (Agriculture) of China(201003020)
; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003)
; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007
; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011)
; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007)
; Sichuan Province Research Foundation for Basic Research(2013JY0082)
; 2013NC0045)
Manganese deficiency or excess caused the depression of intestinal immunity, induction of inflammation and dysfunction of the intestinal physical barrier.pdf（1834KB）
Manganese deficiency or excess caused the depression of intestinal immunity, induction of inflammation and dysfunction of the intestinal physical barrier.pdf
