| Manganese deficiency or excess caused the depression of intestinal immunity, induction of inflammation and dysfunction of the intestinal physical barrier, as regulated by NF-kappa B, TOR and Nrf2 signalling, in grass carp (Ctenopharyngodon idella) | |
| Jiang, Wei-Dan1,2,3; Tang, Ren-Jun1; Liu, Yang1,2,3; Kuang, Sheng-Yao4; Jiang, Jun1,2,3; Wu, Pei1,2,3; Zhao, Juan1; Zhang, Yong-An5; Tang, Ling4; Tang, Wu-Neng4; Zhou, Xiao-Qiu1,2,3; Feng, Lin1,2,3 | |
| 2015-10-01 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
 |
| ISSN | 1050-4648 |
| Volume | 46Issue:2Pages:406-416 |
| Abstract | Intestinal mucosal immune components and mRNA levels of inflammatory cytokines, tight junction proteins, antioxidant enzymes and related signalling molecules in young grass carp (Ctenopharyngodon idellus) under dietary manganese (Mn) deficiency or excess were investigated. Fish were fed the diets containing graded levels of Mn [3.65-27.86 mg Mn kg(-1) diet] for 8 weeks. The results demonstrated that Mn deficiency significantly decreased the lysozyme and acid phosphatase (ACP) activities, up-regulated tumour necrosis factor alpha (TNF-alpha), interleukin 8 and the signalling factor nuclear factor-kappa B p65, and down-regulated interleukin 10 (IL-10), transforming growth factor beta 1, inhibitor of signalling factors kappa B-alpha and target of rapamycin mRNA levels in the proximal intestine (PI), mid intestine (MI) and distal intestine (DI). However, Mn deficiency did not change the C3 content in the PI, whereas it decreased the C3 contents in the MI and DI. Additionally, Mn depletion also resulted in significantly low mRNA levels for tight junction proteins (claudin-b, claudin-c, claudin-15, occludin and zonula occludens-1), antioxidant enzymes (MnSOD, GPx and CAT) and NF-E2-related factor-2 in the intestines of fish. Excessive Mn exhibited toxic effects similar to Mn deficiency, where optimal Mn contents reversed those indicators. In conclusion, Mn deficiency or excess causes the depression of intestinal immunity, induction of inflammation and dysfunction of the intestinal physical barrier relating to NF-kappa B, TOR and Nrf2 signalling in grass carp. Furthermore, quadratic regression analysis at 95% maximum response of lysozyme and acid phosphatase activities in the distal intestine of young grass carp revealed the optimum dietary Mn levels to be 8.90 and 8.99 mg kg(-1) diet, respectively. (C) 2015 Elsevier Ltd. All rights reserved. |
| Subtype | Article |
| Keyword | Manganese Cytokine Tight Junction Antioxidant Status Tor Nrf2 |
| DOI | 10.1016/j.fsi.2015.06.007 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045) |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS ID | WOS:000363071200028 |
| WOS Keyword | SALMON SALMO-SALAR ; TIGHT JUNCTION PERMEABILITY ; TROUT ONCORHYNCHUS-MYKISS ; ATLANTIC SALMON ; DIETARY MANGANESE ; GENE-EXPRESSION ; MITOCHONDRIAL DYSFUNCTION ; AEROMONAS-SALMONICIDA ; OREOCHROMIS-NILOTICUS ; TRANSCRIPT ABUNDANCE |
| Funding Organization | National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045) |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/27329 |
| Collection | 水生生物分子与细胞生物学研究中心_期刊论文 |
| Affiliation | 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China 2.Sichuan Agr Univ, Fish Nutr & Safety Prod Sichuan Univ Key Lab, Chengdu 611130, Sichuan, Peoples R China 3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China 4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Jiang, Wei-Dan,Tang, Ren-Jun,Liu, Yang,et al. Manganese deficiency or excess caused the depression of intestinal immunity, induction of inflammation and dysfunction of the intestinal physical barrier, as regulated by NF-kappa B, TOR and Nrf2 signalling, in grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2015,46(2):406-416. |
| APA | Jiang, Wei-Dan.,Tang, Ren-Jun.,Liu, Yang.,Kuang, Sheng-Yao.,Jiang, Jun.,...&Feng, Lin.(2015).Manganese deficiency or excess caused the depression of intestinal immunity, induction of inflammation and dysfunction of the intestinal physical barrier, as regulated by NF-kappa B, TOR and Nrf2 signalling, in grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,46(2),406-416. |
| MLA | Jiang, Wei-Dan,et al."Manganese deficiency or excess caused the depression of intestinal immunity, induction of inflammation and dysfunction of the intestinal physical barrier, as regulated by NF-kappa B, TOR and Nrf2 signalling, in grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 46.2(2015):406-416. |
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