IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Generation of an Enhancer-Trapping Vector for Insertional Mutagenesis in Zebrafish
Liu, Chunyan1,2,3; Song, Guili1,2; Mao, Lin1,2,3; Long, Yong1,2; Li, Qing1,2; Cui, Zongbin1,2
2015-10-05
Source PublicationPLOS ONE
ISSN1932-6203
Volume10Issue:10Pages:21
Abstract

Enhancer trapping (ET) is a powerful approach to establish tissue-or cell-specific reporters and identify expression patterns of uncharacterized genes. Although a number of enhancer-trapping vectors have been developed and a large library of fish lines with distinct tissue- or cell-specific expression of reporter genes have been generated, the specificity and efficiency of trapping vectors need to be improved because of the bias interaction of minimal promoters with genomic enhancers. Accordingly, we generated an enhancer-trapping vector pTME that contains a minimal mouse metallothionein gene (mMTI) promoter upstream of EGFP reporter. In the first round of screening, twelve zebrafish lines that carry a single copy of ET cassettes were characterized to have tissue-or cell-specific EGFP expression. One of the highly conserved noncoding elements near an insertion site of trapping cassettes was characterized as an enhancer that can specifically regulate the expression of EGFP in cells of the central nervous system. In addition, the pTME vector contains a mutation-cassette that is able to effectively block the transcription of an endogenous gene in an ET line with ubiquitous EGFP expression. Thus, the pTME vector can be used as an alternative tool for both enhancer trapping and mutagenesis across a target genome.

SubtypeArticle
DOI10.1371/journal.pone.0139612
WOS HeadingsScience & Technology
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000362499200036
WOS KeywordCONSERVED NONCODING SEQUENCES ; SLEEPING-BEAUTY TRANSPOSON ; IN-SITU HYBRIDIZATION ; TRANSCRIPTION FACTOR ; GENE-EXPRESSION ; GENOME ; VIVO ; DROSOPHILA ; DISSECTION ; MUTATIONS
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27272
Collection水生生物分子与细胞生物学研究中心_期刊论文
Affiliation1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Liu, Chunyan,Song, Guili,Mao, Lin,et al. Generation of an Enhancer-Trapping Vector for Insertional Mutagenesis in Zebrafish[J]. PLOS ONE,2015,10(10):21.
APA Liu, Chunyan,Song, Guili,Mao, Lin,Long, Yong,Li, Qing,&Cui, Zongbin.(2015).Generation of an Enhancer-Trapping Vector for Insertional Mutagenesis in Zebrafish.PLOS ONE,10(10),21.
MLA Liu, Chunyan,et al."Generation of an Enhancer-Trapping Vector for Insertional Mutagenesis in Zebrafish".PLOS ONE 10.10(2015):21.
Files in This Item:
File Name/Size DocType Version Access License
journal.pone.0139612(3390KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu, Chunyan]'s Articles
[Song, Guili]'s Articles
[Mao, Lin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu, Chunyan]'s Articles
[Song, Guili]'s Articles
[Mao, Lin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu, Chunyan]'s Articles
[Song, Guili]'s Articles
[Mao, Lin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: journal.pone.0139612.PDF
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.