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Ca2+-regulated cyclic electron flow supplies ATP for nitrogen starvation-induced lipid biosynthesis in green alga
Chen, Hui1; Hu, Jinlu1,2; Qiao, Yaqin1,2; Chen, Weixian1,2; Rong, Junfeng3; Zhang, Yunming1,2; He, Chenliu1; Wang, Qiang1
2015-10-09
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume5
AbstractWe previously showed that both the linear photosynthetic electron transportation rate and the respiration rate dropped significantly during N starvation-induced neutral lipid accumulation in an oil-producing microalga, Chlorella sorokiniana, and proposed a possible role for cyclic electron flow (CEF) in ATP supply. In this study, we further exploited this hypothesis in both Chlorella sorokiniana C3 and the model green alga Chlamydomonas. We found that both the rate of CEF around photosystem I and the activity of thylakoid membrane-located ATP synthetase increased significantly during N starvation to drive ATP production. Furthermore, we demonstrated that the Chlamydomonas mutant pgrl1, which is deficient in PGRL1-mediated CEF, accumulated less neutral lipids and had reduced rates of CEF under N starvation. Further analysis revealed that Ca2+ signaling regulates N starvation-induced neutral lipid biosynthesis in Chlamydomonas by increasing calmodulin activity and boosting the expression of the calcium sensor protein that regulates Pgrl1-mediated CEF. Thus, Ca2+-regulated CEF supplies ATP for N starvation-induced lipid biosynthesis in green alga. The increased CEF may re-equilibrate the ATP/NADPH balance and recycle excess light energy in photosystems to prevent photooxidative damage, suggesting Ca2+-regulated CEF also played a key role in protecting and sustaining photosystems.
SubtypeArticle
DOI10.1038/srep15117
WOS HeadingsScience & Technology
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS KeywordCHLORELLA SP C2 ; CHLAMYDOMONAS-REINHARDTII ; PHOTOSYSTEM-I ; TRIACYLGLYCEROL ACCUMULATION ; PLANTS ; PHOTOSYNTHESIS ; TRANSCRIPTOME ; SPECIFICITY ; ARABIDOPSIS ; METABOLISM
WOS IDWOS:000362483500001
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27268
Collection藻类生物学及应用研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.SINOPEC Res Inst Petr Proc, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Chen, Hui,Hu, Jinlu,Qiao, Yaqin,et al. Ca2+-regulated cyclic electron flow supplies ATP for nitrogen starvation-induced lipid biosynthesis in green alga[J]. SCIENTIFIC REPORTS,2015,5.
APA Chen, Hui.,Hu, Jinlu.,Qiao, Yaqin.,Chen, Weixian.,Rong, Junfeng.,...&Wang, Qiang.(2015).Ca2+-regulated cyclic electron flow supplies ATP for nitrogen starvation-induced lipid biosynthesis in green alga.SCIENTIFIC REPORTS,5.
MLA Chen, Hui,et al."Ca2+-regulated cyclic electron flow supplies ATP for nitrogen starvation-induced lipid biosynthesis in green alga".SCIENTIFIC REPORTS 5(2015).
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