中国科学院水生生物研究所机构知识库

Microsatellite markers can provide information on the family structure and genetic diversity based on legible co-dominant Mendel inheritance fashion in diploids. However, parentage and genetics analysis of sturgeon using microsatellite markers is complicated by the polyploidy nature of the species. In the present study, the inheritance mode of microsatellite loci is shown using 3 families created from wild Chinese sturgeon ( Acipenser sinensis). Primers As-073, Spl-168, and As-100 indicated a maximum of four even-intensity bands per individual and pairwise combination of alleles segregation in the progeny that fit tetrasomic inheritance ( P > 0.05). Double reduction exhibited at As-073 simultaneously gave evidence of tetrasomic inheritance. The mode of inheritance at As-074 and Afu-54, both expressing more than two alleles in one individual, could not be ultimately determined because of few inheritance data. The occurrence of null allele at Spl-168 and As-074 was noted.