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The progestin levonorgestrel affects sex differentiation in zebrafish at environmentally relevant concentrations
Hua, Jianghuan1,2; Han, Jian3; Guo, Yongyong1; Zhou, Bingsheng1
2015-09-01
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume166Pages:1-9
AbstractSynthetic progestins have become widespread environmental contaminants and may cause adverse effects on fish. In the present study, we investigated the effects of levonorgestrel (LNG) on sex differentiation in zebrafish (Danio rerio). Embryos were exposed to LNG at environmentally relevant concentrations (0, 1, 10, 33, and 100 ng/L) and allowed to develop until sexual maturity. Histological examination at 63 days post fertilization (dpf) caused complete sex reversal and 100% males were observed in the 10,33 and 100 ng/L treatments; gross morphological and histological examination of gonads at 142 dpf further confirmed 100% males at these exposure concentrations. The results indicate androgenic activity of LNG, and masculinization during zebrafish gonadal differentiation. The mRNA expression levels of genes involved in fish sex differentiation and gonadal development were examined at 28 and 42 dpf. Down-regulation of the mRNA expression of aromatase (e.g., cyp19a1 a, cyp19a1b), the forkhead transcription factor gene L2 (foxl2) and the Fushi tarazu factor-1d (nr5a1b) were observed. In contrast, transcription of the doublesex and mab-3-related transcription factor 1 (dmrt1) gene was up-regulated. Androgen receptor (ar) mRNA expression was significantly down-regulated at 28 and 42 dpf. Co-exposure to flutamide (an androgen antagonist) and LNG, led to a decrease in the sex inversion potency of LNG. Our study has demonstrated that environmentally relevant concentrations of LNG could alter sex differentiation and gonadal development in zebrafish. Our results also suggest a potentially high ecological risk of LNG to fish populations in LNG-contaminated aquatic environments. (C) 2015 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordProgestin Levonorgestrel Sex Differentiation Sex Ratio Androgenic Activity Zebrafish
DOI10.1016/j.aquatox.2015.06.013
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000361256600001
WOS KeywordMINNOW PIMEPHALES-PROMELAS ; STICKLEBACK GASTEROSTEUS-ACULEATUS ; ADVERSELY AFFECT REPRODUCTION ; AROMATASE GENE-TRANSCRIPTION ; DANIO-RERIO ; SYNTHETIC PROGESTINS ; OVARIAN-FOLLICLES ; BINARY-MIXTURES ; XENOPUS-LAEVIS ; FOXL2 GENE
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27210
Collection水环境工程研究中心_期刊论文
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Shangdong Acad Sci, Inst Biol, Jinan 250014, Peoples R China
Recommended Citation
GB/T 7714
Hua, Jianghuan,Han, Jian,Guo, Yongyong,et al. The progestin levonorgestrel affects sex differentiation in zebrafish at environmentally relevant concentrations[J]. AQUATIC TOXICOLOGY,2015,166:1-9.
APA Hua, Jianghuan,Han, Jian,Guo, Yongyong,&Zhou, Bingsheng.(2015).The progestin levonorgestrel affects sex differentiation in zebrafish at environmentally relevant concentrations.AQUATIC TOXICOLOGY,166,1-9.
MLA Hua, Jianghuan,et al."The progestin levonorgestrel affects sex differentiation in zebrafish at environmentally relevant concentrations".AQUATIC TOXICOLOGY 166(2015):1-9.
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