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题名: The progestin levonorgestrel affects sex differentiation in zebrafish at environmentally relevant concentrations
作者: Hua, Jianghuan1, 2; Han, Jian3; Guo, Yongyong1; Zhou, Bingsheng1
关键词: Progestin ; Levonorgestrel ; Sex differentiation ; Sex ratio ; Androgenic activity ; Zebrafish
刊名: AQUATIC TOXICOLOGY
发表日期: 2015-09-01
DOI: 10.1016/j.aquatox.2015.06.013
卷: 166, 页:1-9
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Marine & Freshwater Biology ; Toxicology
研究领域[WOS]: Marine & Freshwater Biology ; Toxicology
英文摘要: Synthetic progestins have become widespread environmental contaminants and may cause adverse effects on fish. In the present study, we investigated the effects of levonorgestrel (LNG) on sex differentiation in zebrafish (Danio rerio). Embryos were exposed to LNG at environmentally relevant concentrations (0, 1, 10, 33, and 100 ng/L) and allowed to develop until sexual maturity. Histological examination at 63 days post fertilization (dpf) caused complete sex reversal and 100% males were observed in the 10,33 and 100 ng/L treatments; gross morphological and histological examination of gonads at 142 dpf further confirmed 100% males at these exposure concentrations. The results indicate androgenic activity of LNG, and masculinization during zebrafish gonadal differentiation. The mRNA expression levels of genes involved in fish sex differentiation and gonadal development were examined at 28 and 42 dpf. Down-regulation of the mRNA expression of aromatase (e.g., cyp19a1 a, cyp19a1b), the forkhead transcription factor gene L2 (foxl2) and the Fushi tarazu factor-1d (nr5a1b) were observed. In contrast, transcription of the doublesex and mab-3-related transcription factor 1 (dmrt1) gene was up-regulated. Androgen receptor (ar) mRNA expression was significantly down-regulated at 28 and 42 dpf. Co-exposure to flutamide (an androgen antagonist) and LNG, led to a decrease in the sex inversion potency of LNG. Our study has demonstrated that environmentally relevant concentrations of LNG could alter sex differentiation and gonadal development in zebrafish. Our results also suggest a potentially high ecological risk of LNG to fish populations in LNG-contaminated aquatic environments. (C) 2015 Elsevier B.V. All rights reserved.
关键词[WOS]: MINNOW PIMEPHALES-PROMELAS ; STICKLEBACK GASTEROSTEUS-ACULEATUS ; ADVERSELY AFFECT REPRODUCTION ; AROMATASE GENE-TRANSCRIPTION ; DANIO-RERIO ; SYNTHETIC PROGESTINS ; OVARIAN-FOLLICLES ; BINARY-MIXTURES ; XENOPUS-LAEVIS ; FOXL2 GENE
语种: 英语
WOS记录号: WOS:000361256600001
ISSN号: 0166-445X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27210
Appears in Collections:水环境工程研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Shangdong Acad Sci, Inst Biol, Jinan 250014, Peoples R China
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