Ca2+ and caspases are involved in hydroxyl radical-induced apoptosis in erythrocytes of Jian carp (Cyprinus carpio var. Jian)
Li, HuaTao1,2; Feng, Lin1,3,4; Jiang, WeiDan1,3,4; Liu, Yang1,3,4; Jiang, Jun1,3,4; Zhang, YongAn5; Wu, Pei1,3,4; Zhou, XiaoQiu1,3,4
2015-10-01
Source PublicationFISH PHYSIOLOGY AND BIOCHEMISTRY
ISSN0920-1742
Volume41Issue:5Pages:1305-1319
AbstractThere are young erythrocytes and mature erythrocytes in the peripheral blood of fish. The present study explored the apoptosis in hydroxyl radical ((OH)-O-center dot)-induced young and mature erythrocytes of Jian carp (Cyprinus carpio var. Jian). Carp erythrocytes from the peripheral blood were separated into the young fraction, the intermediate fraction and the mature fraction using fixed-angle centrifugation. The erythrocytes in three age fractions were treated with the caspase inhibitors (zVAD-fmk) in physiological carp saline (PCS) or Ca2+-free PCS in the presence of 40 mu M FeSO4/20 mu M H2O2. The results showed that the (OH)-O-center dot-induced reactive oxygen species (ROS) generation, phosphatidylserine (PS) exposure and DNA fragmentation are caspase dependent in carp erythrocytes. Furthermore, the ROS generation, PS exposure and DNA fragmentation in the more young fraction are more dependent on the caspase activity. This suggested that the caspases are involved in the (OH)-O-center dot-induced apoptosis in the young erythrocytes of fish. Results also indicated that Ca2+ is involved in (OH)-O-center dot-induced calpain activation, PS exposure and DNA fragmentation in carp erythrocytes. Moreover, the calpain activation, DNA fragmentation and PS exposure in the more mature fraction are more dependent on the levels of Ca2+. This revealed that (OH)-O-center dot-induced apoptosis is Ca2+ dependent in the mature erythrocytes of fish. Taken together, there might be two apoptosis pathways in fish erythrocytes: one is the caspase-dependent apoptosis in the young erythrocytes and the other is the Ca2+-involved apoptosis in the mature erythrocytes.
SubtypeArticle
KeywordFish Erythrocyte Apoptosis Ca2++ Caspase Ps Exposure Dna Fragmentation
DOI10.1007/s10695-015-0087-0
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Fisheries ; Physiology
WOS SubjectBiochemistry & Molecular Biology ; Fisheries ; Physiology
WOS KeywordRED-BLOOD-CELLS ; OXIDATIVE STRESS ; HYDROGEN-PEROXIDE ; PHOSPHATIDYLSERINE EXTERNALIZATION ; CHALCALBURNUS-TARICHI ; BID CLEAVAGE ; CANCER-CELLS ; LAKE VAN ; DEATH ; FISH
WOS IDWOS:000361539100017
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27207
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
2.Neijiang Normal Univ, Conservat & Utilizat Fishes Resources Upper Reach, Neijiang 641000, Sichuan, Peoples R China
3.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Agr Univ, Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Li, HuaTao,Feng, Lin,Jiang, WeiDan,et al. Ca2+ and caspases are involved in hydroxyl radical-induced apoptosis in erythrocytes of Jian carp (Cyprinus carpio var. Jian)[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2015,41(5):1305-1319.
APA Li, HuaTao.,Feng, Lin.,Jiang, WeiDan.,Liu, Yang.,Jiang, Jun.,...&Zhou, XiaoQiu.(2015).Ca2+ and caspases are involved in hydroxyl radical-induced apoptosis in erythrocytes of Jian carp (Cyprinus carpio var. Jian).FISH PHYSIOLOGY AND BIOCHEMISTRY,41(5),1305-1319.
MLA Li, HuaTao,et al."Ca2+ and caspases are involved in hydroxyl radical-induced apoptosis in erythrocytes of Jian carp (Cyprinus carpio var. Jian)".FISH PHYSIOLOGY AND BIOCHEMISTRY 41.5(2015):1305-1319.
Files in This Item:
File Name/Size DocType Version Access License
Ca2+ and caspases ar(735KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, HuaTao]'s Articles
[Feng, Lin]'s Articles
[Jiang, WeiDan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, HuaTao]'s Articles
[Feng, Lin]'s Articles
[Jiang, WeiDan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, HuaTao]'s Articles
[Feng, Lin]'s Articles
[Jiang, WeiDan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Ca2+ and caspases are involved in hydroxyl radical-induced apoptosis in erythrocytes of Jian carp (Cyprinus carpio var. Jian).pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.