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题名: Ca2+ and caspases are involved in hydroxyl radical-induced apoptosis in erythrocytes of Jian carp (Cyprinus carpio var. Jian)
作者: Li, HuaTao1, 2; Feng, Lin1, 3, 4; Jiang, WeiDan1, 3, 4; Liu, Yang1, 3, 4; Jiang, Jun1, 3, 4; Zhang, YongAn5; Wu, Pei1, 3, 4; Zhou, XiaoQiu1, 3, 4
关键词: Fish erythrocyte ; Apoptosis ; Ca2+ ; Caspase ; PS exposure ; DNA fragmentation
刊名: FISH PHYSIOLOGY AND BIOCHEMISTRY
发表日期: 2015-10-01
DOI: 10.1007/s10695-015-0087-0
卷: 41, 期:5, 页:1305-1319
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Fisheries ; Physiology
研究领域[WOS]: Biochemistry & Molecular Biology ; Fisheries ; Physiology
英文摘要: There are young erythrocytes and mature erythrocytes in the peripheral blood of fish. The present study explored the apoptosis in hydroxyl radical ((OH)-O-center dot)-induced young and mature erythrocytes of Jian carp (Cyprinus carpio var. Jian). Carp erythrocytes from the peripheral blood were separated into the young fraction, the intermediate fraction and the mature fraction using fixed-angle centrifugation. The erythrocytes in three age fractions were treated with the caspase inhibitors (zVAD-fmk) in physiological carp saline (PCS) or Ca2+-free PCS in the presence of 40 mu M FeSO4/20 mu M H2O2. The results showed that the (OH)-O-center dot-induced reactive oxygen species (ROS) generation, phosphatidylserine (PS) exposure and DNA fragmentation are caspase dependent in carp erythrocytes. Furthermore, the ROS generation, PS exposure and DNA fragmentation in the more young fraction are more dependent on the caspase activity. This suggested that the caspases are involved in the (OH)-O-center dot-induced apoptosis in the young erythrocytes of fish. Results also indicated that Ca2+ is involved in (OH)-O-center dot-induced calpain activation, PS exposure and DNA fragmentation in carp erythrocytes. Moreover, the calpain activation, DNA fragmentation and PS exposure in the more mature fraction are more dependent on the levels of Ca2+. This revealed that (OH)-O-center dot-induced apoptosis is Ca2+ dependent in the mature erythrocytes of fish. Taken together, there might be two apoptosis pathways in fish erythrocytes: one is the caspase-dependent apoptosis in the young erythrocytes and the other is the Ca2+-involved apoptosis in the mature erythrocytes.
关键词[WOS]: RED-BLOOD-CELLS ; OXIDATIVE STRESS ; HYDROGEN-PEROXIDE ; PHOSPHATIDYLSERINE EXTERNALIZATION ; CHALCALBURNUS-TARICHI ; BID CLEAVAGE ; CANCER-CELLS ; LAKE VAN ; DEATH ; FISH
语种: 英语
WOS记录号: WOS:000361539100017
ISSN号: 0920-1742
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27207
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
2.Neijiang Normal Univ, Conservat & Utilizat Fishes Resources Upper Reach, Neijiang 641000, Sichuan, Peoples R China
3.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Agr Univ, Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
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