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题名: Expression characterization, genomic structure and function analysis of fish ubiquitin-specific protease 18 (USP18) genes
作者: Chen, Chen1; Zhang, Yi-Bing1; Gui, Jian-Fang1
关键词: Ubiquitin-specific protease 18 ; Interferon-stimulated gene ; Expression regulation ; Subcellular localization ; Promoter analysis
刊名: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
发表日期: 2015-10-01
DOI: 10.1016/j.dci.2015.05.003
卷: 52, 期:2, 页:112-122
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Immunology ; Zoology
研究领域[WOS]: Immunology ; Zoology
英文摘要: In mammals, USP18 (ubiquitin-specific protease 18) is an interferon (IFN) inducible protein and plays a role in regulation of IFN response upon viral infection. In this study, we first cloned a USP18 homologous gene from virally-infected crucian carp (Carassius auratus) blastula embryonic (CAB) cells, and later found in other fish species including zebrafish. All fish USP18 genes have 10 exons and 9 introns comparable toll exons and 10 introns in non-fish vertebrates. Expression analysis revealed that fish USP18 was significantly induced in vitro and in vivo by IFN and IFN stimuli. Using promoter-driven luciferase reporter assay system to explore the molecular mechanism underlying fish USP18 expression, fish USP18 was identified as a typical interferon (IFN)-stimulated gene (ISG). Intracellular poly(I:C)-triggered zebrafish USP18 expression was regulated through RLR-IFN pathway, which was consistent with the fact that fish USP18 gene promoter contained two typical IFN-stimulated response elements (ISREs). Further mutation assays revealed that the distant ISRE motif primarily contributed to the induction of zebrafish USPI8 by fish IFN and IFN stimuli. Functionally, fish USP18 inhibited poly(I:C)- and IFN-triggered activation of a common ISRE-containing promoter, and attenuated transcriptional expression of some ISGs including Stat1 and PKZ by recombinant IFN. Finally, we found that fish USP18 protein was expressed in cytoplasm and exhibited an ability to interact with ISG15. These results indicate that fish USPI8 likely exerts its function similar to mammalian homologs. (C) 2015 Elsevier Ltd. All rights reserved.
关键词[WOS]: C VIRUS-INFECTION ; ANTIVIRAL RESPONSE ; UBP43 USP18 ; AQUACULTURE ANIMALS ; CARASSIUS-AURATUS ; IMMUNE-RESPONSE ; IFN RESPONSE ; INTERFERON ; ISG15 ; CELLS
语种: 英语
WOS记录号: WOS:000360592200002
ISSN号: 0145-305X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27191
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
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