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Expression characterization, genomic structure and function analysis of fish ubiquitin-specific protease 18 (USP18) genes
Chen, Chen; Zhang, Yi-Bing; Gui, Jian-Fang
2015-10-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume52Issue:2Pages:112-122
AbstractIn mammals, USP18 (ubiquitin-specific protease 18) is an interferon (IFN) inducible protein and plays a role in regulation of IFN response upon viral infection. In this study, we first cloned a USP18 homologous gene from virally-infected crucian carp (Carassius auratus) blastula embryonic (CAB) cells, and later found in other fish species including zebrafish. All fish USP18 genes have 10 exons and 9 introns comparable toll exons and 10 introns in non-fish vertebrates. Expression analysis revealed that fish USP18 was significantly induced in vitro and in vivo by IFN and IFN stimuli. Using promoter-driven luciferase reporter assay system to explore the molecular mechanism underlying fish USP18 expression, fish USP18 was identified as a typical interferon (IFN)-stimulated gene (ISG). Intracellular poly(I:C)-triggered zebrafish USP18 expression was regulated through RLR-IFN pathway, which was consistent with the fact that fish USP18 gene promoter contained two typical IFN-stimulated response elements (ISREs). Further mutation assays revealed that the distant ISRE motif primarily contributed to the induction of zebrafish USPI8 by fish IFN and IFN stimuli. Functionally, fish USP18 inhibited poly(I:C)- and IFN-triggered activation of a common ISRE-containing promoter, and attenuated transcriptional expression of some ISGs including Stat1 and PKZ by recombinant IFN. Finally, we found that fish USP18 protein was expressed in cytoplasm and exhibited an ability to interact with ISG15. These results indicate that fish USPI8 likely exerts its function similar to mammalian homologs. (C) 2015 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordUbiquitin-specific Protease 18 Interferon-stimulated Gene Expression Regulation Subcellular Localization Promoter Analysis
DOI10.1016/j.dci.2015.05.003
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS KeywordC VIRUS-INFECTION ; ANTIVIRAL RESPONSE ; UBP43 USP18 ; AQUACULTURE ANIMALS ; CARASSIUS-AURATUS ; IMMUNE-RESPONSE ; IFN RESPONSE ; INTERFERON ; ISG15 ; CELLS
WOS IDWOS:000360592200002
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27191
Collection鱼类生物学及渔业生物技术研究中心
AffiliationUniv Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Chen, Chen,Zhang, Yi-Bing,Gui, Jian-Fang. Expression characterization, genomic structure and function analysis of fish ubiquitin-specific protease 18 (USP18) genes[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,52(2):112-122.
APA Chen, Chen,Zhang, Yi-Bing,&Gui, Jian-Fang.(2015).Expression characterization, genomic structure and function analysis of fish ubiquitin-specific protease 18 (USP18) genes.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,52(2),112-122.
MLA Chen, Chen,et al."Expression characterization, genomic structure and function analysis of fish ubiquitin-specific protease 18 (USP18) genes".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 52.2(2015):112-122.
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