中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
题名: Direct production of XYDMY- sex reversal female medaka (Oryzias latipes) by embryo microinjection of TALENs
作者: Luo, Daji1, 2; Liu, Yun3; Chen, Ji2; Xia, Xiaoqin2; Cao, Mengxi2; Cheng, Bin1; Wang, Xuejuan1; Gong, Wuming4; Qiu, Chao1; Zhang, Yunsheng2; Cheng, Christopher Hon Ki3; Zhu, Zuoyan2; Hu, Wei2
刊名: SCIENTIFIC REPORTS
发表日期: 2015-09-14
DOI: 10.1038/srep14057
卷: 5
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: Medaka is an ideal model for sex determination and sex reversal, such as XY phenotypically female patients in humans. Here, we assembled improved TALENs targeting the DMY gene and generated XYDMY- mutants to investigate gonadal dysgenesis in medaka. DMY-TALENs resulted in indel mutations at the targeted loci (46.8%). DMY-nanos3UTR-TALENs induced mutations were passed through the germline to F1 generation with efficiencies of up to 91.7%. XYDMY- mutants developed into females, laid eggs, and stably passed the YDMY- chromosome to next generation. RNA-seq generated 157 million raw reads from WT male (WT_M_TE), WT female (WT_F_OV) and XYDMY- female medaka (TA_F_OV) gonad libraries. Differential expression analysis identified 144 up-and 293 down-regulated genes in TA_F_OV compared with WT_F_OV, 387 up-and 338 down-regulated genes in TA_F_OV compared with WT_ M_ TE. According to genes annotation and functional prediction, such as Wnt1 and PRCK, it revealed that incomplete ovarian function and reduced fertility of XYDMY- mutant is closely related to the wnt signaling pathway. Our results provided the transcriptional profiles of XYDMY- mutants, revealed the mechanism between sex reversal and DMY in medaka, and suggested that XYDMY- medaka was a novel mutant that is useful for investigating gonadal dysgenesis in phenotypic female patients with the 46, XY karyotype.
关键词[WOS]: TARGETED GENE DISRUPTION ; EFFECTOR NUCLEASES ; DETERMINING REGION ; PHENOTYPIC FEMALE ; CLEAVAGE DOMAIN ; Y-CHROMOSOME ; FISH ; ZEBRAFISH ; DMRT1 ; DMY
语种: 英语
WOS记录号: WOS:000361149600001
ISSN号: 2045-2322
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27190
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Direct production of XYDMY- sex reversal female medaka (Oryzias latipes) by embryo microinjection of TALENs.pdf(2555KB)----开放获取--View Download

作者单位: 1.Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430071, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
3.Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China
4.Univ Minnesota, Lillehei Heart Inst, Minneapolis, MN USA
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Luo, Daji]'s Articles
[Liu, Yun]'s Articles
[Chen, Ji]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Luo, Daji]‘s Articles
[Liu, Yun]‘s Articles
[Chen, Ji]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Direct production of XYDMY- sex reversal female medaka (Oryzias latipes) by embryo microinjection of TALENs.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace