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题名: Functional characterizations of IPS-1 in CIK cells: Potential roles in regulating IFN-I response dependent on IRF7 but not IRF3
作者: Feng, Xiaoli1; Zhang, Yixuan1; Yang, Chunrong2; Liao, Lanjie3; Wang, Yaping3; Su, Jianguo1, 4, 5
关键词: Grass carp (Ctenopharyngodon idella) ; IPS-1 ; Functional characteristics ; Grass carp reovirus
刊名: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
发表日期: 2015-11-01
DOI: 10.1016/j.dc12015.06.005
卷: 53, 期:1, 页:23-32
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Immunology ; Zoology
研究领域[WOS]: Immunology ; Zoology
英文摘要: IPS-1, as the sole adaptor of RIG-I and MDA5, plays a central role in innate antiviral immunity. In this study, we investigated potential roles of IPS-1 in innate immunity and the domain-requirement of IPS-1 for its signaling in grass carp (Ctenopharyngodon idella). Overexpression experiment showed that CiIPS-1 mediated IFN-I signal possibly dependent on CiIRF7 but not CiIRF3. Post GCRV challenge, CiIPS-1 could enhance antiviral immune responses. CARD and TM domains were crucial for antiviral function of CiIPS-1, and TRAF motif played an assistant role. PRO domain seemed as a negative regulator but was pivotal for the initiation of CiIFN-I and CiMx1. Post viral/bacterial PAMPs stimulation, CiIPS-1-mediated signaling was tightly controlled. CARD domain of CiIPS-1 could significantly elicit poly I:C/LPS/PGN-mediated signaling. PRO domain negatively regulated CiIRF7 and CiIFN-I but was indispensable for inductions of CiMx1 and CiIL-1 beta. TRAF motif and TM domain regulated the signaling presumably in a cooperative fashion. Post poly I:C stimulation, TRAF motif negatively regulated CiIRF7, CiIFN-I and CiIL-1 beta at a relative early time while TM domain functioned at a relative late time. TRAF motif was indispensable for the production of CiMx1, while TM domain slightly negatively regulated the expression. Post LPS and PGN stimulation, TRAF motif excited an assistant and persistent negative role on CiIFN-I, CiIRF7 and CiIL-1 beta induction, but was crucial for induction of CiMx1. TM domain slightly negatively regulated LPS- and PGN-triggered signaling. Taken together, CiIPS-1 not only exerted important functions in antiviral immune response but also participated in viral/bacterial PAMPs-triggered immune response which was tightly controlled to prevent harmful effects resulting from excessive activation. This study provided novel insights into the pivotal role of IFS-1 in innate immunity. (C) 2015 Elsevier Ltd. All rights reserved.
关键词[WOS]: INTERFERON ANTIVIRAL RESPONSE ; INNATE IMMUNE-RESPONSES ; MAVS SPLICING VARIANTS ; NF-KAPPA-B ; VIRAL/BACTERIAL PAMPS ; SIGNALING PROTEIN ; RECEPTORS ; INDUCTION ; PATHWAY ; ACTIVATION
语种: 英语
WOS记录号: WOS:000360772900003
ISSN号: 0145-305X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27178
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China
2.Huazhong Agr Univ, Coll Vet Med, Wuhan 430070, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
4.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
5.Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Peoples R China
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