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Functional characterizations of IPS-1 in CIK cells: Potential roles in regulating IFN-I response dependent on IRF7 but not IRF3
Feng, Xiaoli1; Zhang, Yixuan1; Yang, Chunrong2; Liao, Lanjie3; Wang, Yaping3; Su, Jianguo1,4,5
2015-11-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume53Issue:1Pages:23-32
AbstractIPS-1, as the sole adaptor of RIG-I and MDA5, plays a central role in innate antiviral immunity. In this study, we investigated potential roles of IPS-1 in innate immunity and the domain-requirement of IPS-1 for its signaling in grass carp (Ctenopharyngodon idella). Overexpression experiment showed that CiIPS-1 mediated IFN-I signal possibly dependent on CiIRF7 but not CiIRF3. Post GCRV challenge, CiIPS-1 could enhance antiviral immune responses. CARD and TM domains were crucial for antiviral function of CiIPS-1, and TRAF motif played an assistant role. PRO domain seemed as a negative regulator but was pivotal for the initiation of CiIFN-I and CiMx1. Post viral/bacterial PAMPs stimulation, CiIPS-1-mediated signaling was tightly controlled. CARD domain of CiIPS-1 could significantly elicit poly I:C/LPS/PGN-mediated signaling. PRO domain negatively regulated CiIRF7 and CiIFN-I but was indispensable for inductions of CiMx1 and CiIL-1 beta. TRAF motif and TM domain regulated the signaling presumably in a cooperative fashion. Post poly I:C stimulation, TRAF motif negatively regulated CiIRF7, CiIFN-I and CiIL-1 beta at a relative early time while TM domain functioned at a relative late time. TRAF motif was indispensable for the production of CiMx1, while TM domain slightly negatively regulated the expression. Post LPS and PGN stimulation, TRAF motif excited an assistant and persistent negative role on CiIFN-I, CiIRF7 and CiIL-1 beta induction, but was crucial for induction of CiMx1. TM domain slightly negatively regulated LPS- and PGN-triggered signaling. Taken together, CiIPS-1 not only exerted important functions in antiviral immune response but also participated in viral/bacterial PAMPs-triggered immune response which was tightly controlled to prevent harmful effects resulting from excessive activation. This study provided novel insights into the pivotal role of IFS-1 in innate immunity. (C) 2015 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordGrass Carp (Ctenopharyngodon Idella) Ips-1 Functional Characteristics Grass Carp Reovirus
DOI10.1016/j.dc12015.06.005
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS KeywordINTERFERON ANTIVIRAL RESPONSE ; INNATE IMMUNE-RESPONSES ; MAVS SPLICING VARIANTS ; NF-KAPPA-B ; VIRAL/BACTERIAL PAMPS ; SIGNALING PROTEIN ; RECEPTORS ; INDUCTION ; PATHWAY ; ACTIVATION
WOS IDWOS:000360772900003
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27178
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China
2.Huazhong Agr Univ, Coll Vet Med, Wuhan 430070, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
4.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
5.Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Feng, Xiaoli,Zhang, Yixuan,Yang, Chunrong,et al. Functional characterizations of IPS-1 in CIK cells: Potential roles in regulating IFN-I response dependent on IRF7 but not IRF3[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,53(1):23-32.
APA Feng, Xiaoli,Zhang, Yixuan,Yang, Chunrong,Liao, Lanjie,Wang, Yaping,&Su, Jianguo.(2015).Functional characterizations of IPS-1 in CIK cells: Potential roles in regulating IFN-I response dependent on IRF7 but not IRF3.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,53(1),23-32.
MLA Feng, Xiaoli,et al."Functional characterizations of IPS-1 in CIK cells: Potential roles in regulating IFN-I response dependent on IRF7 but not IRF3".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 53.1(2015):23-32.
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