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Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques
Li, Huan1; Zhong, Qiuping1; Wirth, Stephan2; Wang, Weiwei1; Hao, Yaotong1; Wu, Shangong1; Zou, Hong1; Li, Wenxiang1; Wang, Guitang1
2015-10-01
Source PublicationAQUACULTURE RESEARCH
ISSN1355-557X
Volume46Issue:10Pages:2344-2359
AbstractTraditional culture-based technique and 16S rDNA sequencing method were used to investigate the mucosa-associated autochthonous microbiota of grass carp (Ctenopharyngodon idellus). Twenty-one phylotypes were detected from culturable microbiota, with Aeromonas, Shewanella, Lactococcus, Serratia, Brevibacillus, Delftia, Pseudomonas, Pantoea, Enterobacter, Buttiauxella and Yersinia as their closest relatives. Genomic DNA was directly extracted from the gut mucosa of C. idellus originating from six different geographical regions, and used to generate 609 random bacterial clones from six clone libraries and 99 archaeal clones from one library, which were grouped into 67 bacterial and four archaeal phylotypes. Sequence analysis revealed that the intestinal mucosa harboured a diversified bacterial microbiota, where Proteobacteria, Firmicutes and Bacteroidetes were dominant, followed by Actinobacteria, Verrucomicrobia and Deinococcus-Thermus. The autochthonous bacterial communities in the gut mucosa of fish from different aquatic environments were not similar (C-s < 0.80), but gamma-Proteobacteria was a common bacterial class. In comparison to bacterial communities, the archaeal community obtained from one library consisted of Crenarchaeota and Euryarchaeota. These results demonstrate that molecular methods facilitate culture-independent studies, and that fish gut mucosa harbours a larger bacterial diversity than previously recognized. The grass carp intestinal habitat selects for specific bacterial taxa despite pronounced differences in host environments.
SubtypeArticle
KeywordGrass Carp Autochthonous Microbiota Gut Mucosa 16s Rdna
DOI10.1111/are.12391
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries
WOS SubjectFisheries
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; COMPLETE GENOME SEQUENCE ; 16S RIBOSOMAL-RNA ; MOLECULAR CHARACTERIZATION ; PHYLOGENETIC ANALYSIS ; MICROBIAL DIVERSITY ; GROWTH-PERFORMANCE ; GUT MICROBIOTA ; AUREUS MALE ; FISH
WOS IDWOS:000360671700004
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27174
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Leibniz Ctr Agr Landscape Res ZALF, Inst Landscape Biogeochem, Muncheberg, Germany
Recommended Citation
GB/T 7714
Li, Huan,Zhong, Qiuping,Wirth, Stephan,et al. Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques[J]. AQUACULTURE RESEARCH,2015,46(10):2344-2359.
APA Li, Huan.,Zhong, Qiuping.,Wirth, Stephan.,Wang, Weiwei.,Hao, Yaotong.,...&Wang, Guitang.(2015).Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques.AQUACULTURE RESEARCH,46(10),2344-2359.
MLA Li, Huan,et al."Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques".AQUACULTURE RESEARCH 46.10(2015):2344-2359.
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