IHB OpenIR  > 分析测试中心
A Chitin-Like Component on Sclerotic Cells of Fonsecaea pedrosoi Inhibits Dectin-1-Mediated Murine Th17 Development by Masking beta-Glucans
Dong, Bilin1; Li, Dongsheng1; Li, Ruoyu2; Chen, Sharon C. -A.3; Liu, Weihuang4; Liu, Wei5; Chen, Liuqing1; Chen, Yao1; Zhang, Xu1; Tong, Zhongsheng1; Xia, Yun1; Xia, Ping1; Wang, Yan5; Duan, Yiqun1
2014-12-09
Source PublicationPLOS ONE
ISSN1932-6203
Volume9Issue:12Pages:1-20
AbstractFonsecaea pedrosoi (F. pedrosoi), a major agent of chromoblastomycosis, has been shown to be recognized primarily by C-type lectin receptors (CLRs) in a murine model of chromoblastomycosis. Specifically, the beta-glucan receptor, Dectin-1, mediates Th17 development and consequent recruitment of neutrophils, and is evidenced to have the capacity to bind to saprophytic hyphae of F. pedrosoi in vitro. However, when embedded in tissue, most etiological agents of chromoblastomycosis including F. pedrosoi will transform into the sclerotic cells, which are linked to the greatest survival of melanized fungi in tissue. In this study, using immunocompetent and athymic (nu/nu) murine models infected subcutaneously or intraperitoneally with F. pedrosoi, we demonstrated that T lymphocytes play an active role in the resolution of localized footpad infection, and there existed a significantly decreased expression of Th17-defining transcription factor Ror gamma t and inefficient recruitment of neutrophils in chronically infected spleen where the inoculated mycelium of F. pedrosoi transformed into the sclerotic cells. We also found that Dectin-1-expressing histocytes and neutrophils participated in the enclosure of transformed sclerotic cells in the infectious foci. Furthermore, we induced the formation of sclerotic cells in vitro, and evidenced a significantly decreased binding capacity of human or murine-derived Dectin-1 to the induced sclerotic cells in comparison with the saprophytic mycelial forms. Our analysis of beta-glucans-masking components revealed that it is a chitin-like component, but not the mannose moiety on the sclerotic cells, that interferes with the binding of beta-glucans by human or murine Dectin-1. Notably, we demonstrated that although Dectin-1 contributed to the development of IL-17A-producing CD3+CD4+ murine splenocytes upon in vitro-stimulation by saprophytic F. pedrosoi, the masking effect of chitin components partly inhibited Dectin-1-mediated Th17 development upon in vitro-stimulation by induced sclerotic cells. Therefore, these findings extend our understanding of the chronicity of chromoblastomycosis.
SubtypeArticle
DOI10.1371/journal.pone.0114113
WOS HeadingsScience & Technology
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS KeywordHOST-DEFENSE ; CHROMOBLASTOMYCOSIS ; RECEPTOR ; INFECTION ; IMMUNITY ; FORMS ; RECOGNITION ; CYTOKINES ; AGENT ; FUNGI
WOS IDWOS:000347515300017
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27132
Collection分析测试中心
Corresponding AuthorTong, Zhongsheng; Duan, Yiqun
Affiliation1.1 Hosp Wuhan, Ctr Infect Skin Dis, Dept Dermatol, Wuhan, Peoples R China
2.Peking Univ, Res Ctr Med Mycol, Beijing 100871, Peoples R China
3.Univ Sydney, Westmead Hosp, ICPMR Pathol W, Ctr Infect Dis & Microbiol,Lab Serv, Westmead, NSW 2145, Australia
4.Wuhan Univ, Med Res Ctr, Wuhan 430072, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Dong, Bilin,Li, Dongsheng,Li, Ruoyu,et al. A Chitin-Like Component on Sclerotic Cells of Fonsecaea pedrosoi Inhibits Dectin-1-Mediated Murine Th17 Development by Masking beta-Glucans[J]. PLOS ONE,2014,9(12):1-20.
APA Dong, Bilin.,Li, Dongsheng.,Li, Ruoyu.,Chen, Sharon C. -A..,Liu, Weihuang.,...&Duan, Yiqun.(2014).A Chitin-Like Component on Sclerotic Cells of Fonsecaea pedrosoi Inhibits Dectin-1-Mediated Murine Th17 Development by Masking beta-Glucans.PLOS ONE,9(12),1-20.
MLA Dong, Bilin,et al."A Chitin-Like Component on Sclerotic Cells of Fonsecaea pedrosoi Inhibits Dectin-1-Mediated Murine Th17 Development by Masking beta-Glucans".PLOS ONE 9.12(2014):1-20.
Files in This Item:
File Name/Size DocType Version Access License
journal.pone.0114113(1821KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Dong, Bilin]'s Articles
[Li, Dongsheng]'s Articles
[Li, Ruoyu]'s Articles
Baidu academic
Similar articles in Baidu academic
[Dong, Bilin]'s Articles
[Li, Dongsheng]'s Articles
[Li, Ruoyu]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Dong, Bilin]'s Articles
[Li, Dongsheng]'s Articles
[Li, Ruoyu]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: journal.pone.0114113.PDF
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.