Science & Technology
; Life Sciences & Biomedicine
类目[WOS]:
Marine & Freshwater Biology
; Toxicology
研究领域[WOS]:
Marine & Freshwater Biology
; Toxicology
英文摘要:
This study was conducted to evaluate possible bystander effects induced by the model chemical 6-propyl-2-thiouracil (PTU) on melanin synthesis. Zebrafish (Danio rerio) embryos were treated with PTU by either microinjection exposure, via waterborne exposure or indirectly through bystander exposure. Melanin content, related mRNA and protein expression were examined at the end of exposure (36 h post-fertilization). Direct exposure to FLU decreased the melanin content, up-regulated mRNA expressions of oculocutaneous albinism type 2 (OCA2), tyrosinase (7YR), dopachrometautomerase (DCT), tyrosinase-related protein 1 (TYRP1) and silver (SILV), and increased the protein expressions of TYR and SILV. Bystander exposure also up-regulated mRNA and protein expressions of TYR and SILV but increased melanin contents. Correlation analysis demonstrated that mRNA expressions of OCA2, TYR, DCT, TYRP1, SILV and protein expressions of TYR and SILV in bystander exposure groups were positively correlated with corresponding expressions in microinjection exposure groups. The results might have environmental implications and highlight the need to consider the bystander effects when assessing potential risks of endocrine-disrupting chemicals. (C) 2012 Elsevier B.V. All rights reserved.
1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China 2.Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing 210093, Peoples R China 3.Nanjing Univ, Sch Environm, Nanjing 210093, Peoples R China 4.Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Wuhan 430064, Hubei, Peoples R China 5.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 6.Univ Saskatchewan, Dept Vet Biomed Sci, Saskatoon, SK, Canada 7.Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK, Canada 8.City Univ Hong Kong, Dept Biol & Chem, Kowloon, Hong Kong, Peoples R China 9.City Univ Hong Kong, State Key Lab Marine Pollut, Kowloon, Hong Kong, Peoples R China
