Science & Technology
; Life Sciences & Biomedicine
Bacteria from 30 duck liver samples were characterized using VITEK System. Two strains showed 99% similarity to Edwardsiella tarda (E. tarda). A set of seven virulence genes viz. citC (citrate lyaseligase), fimA (fimbrial operon), gadB (glutamate decarboxylase), katB (catalase), mukF (putative killing factor), esrB (TTSS regulator) and gyrB (ATPase domain of DNA gyrase) were amplified from obtained duck E. tarda and reference fish E. tarda. The results obtained show that all seven genes were present in the isolate X1 and reference strain, while only gyrB, citC and mukF genes were present in the isolate X2. The results obtained also indicate that X1, X2, and reference strain were all virulence strains.
1.Zhejiang Mariculture Res Inst, Zhejiang Key Lab Exploitat & Preservat Coastal Bi, Wenzhou 325005, Peoples R China 2.Shandong Agr Univ, Coll Anim Sci & Technol, Tai An 271018, Shandong, Peoples R China 3.Chinese Acad Sci, Huaian Res Ctr, Inst Hydrobiol, Huaian 223002, Peoples R China