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题名: Identification and characterization of MAVS from black carp Mylopharyngodon piceus
作者: Zhou, Wei1; Zhou, Jujun1; Lv, Ying1; Qu, Yixiao1; Chi, Mengdie1; Li, Jun1; Feng, Hao1, 2
关键词: Black carp ; MAVS ; Innate immunity
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2015-04-01
DOI: 10.1016/j.fsi.2015.01.016
卷: 43, 期:2, 页:460-468
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
英文摘要: MAVS (mitochondria antiviral signaling protein) plays an important role in the host cellular innate immune response against microbial pathogens. In this study, MAVS has been cloned and characterized from black carp (Mylopharyngodon piceus). The full-length cDNA of black carp MAVS (bcMAVS) consists of 2352 nucleotides and the predicted bcMAVS protein contains 579 amino acids. Structural analysis showed that bcMAVS is composed of functional domains including an N-terminal CARD, a central proline-rich domain, a putative TRAF2-binding motif and a C-terminal TM domain, which is similar to mammalian MAVS. bcMAVS is constitutively transcribed in all the selected tissues including gill, kidney, heart, intestine, liver, muscle, skin and spleen; bcMAVS mRNA level in intestine, liver, muscle increased but decreased in spleen right after GCRV or SVCV infection. Multiple bands of bcMAVS were detected in western blot when it was expressed in tissue culture, which is similar to mammalian MAVS. Immunofluorescence assay determined that bcMAVS is a mitochondria protein and luciferase reporter assay demonstrated that bcMAVS could induce zebrafish IFN and EPC IFN expression in tissue culture. Data generated in this manuscript has built a solid foundation for further elucidating the function of bcMAVS in the innate immune system of black carp. (C) 2015 Elsevier Ltd. All rights reserved.
关键词[WOS]: ANTIVIRAL SIGNALING PROTEIN ; TOLL-LIKE RECEPTORS ; I-LIKE RECEPTORS ; NF-KAPPA-B ; RIG-I ; ADAPTER PROTEIN ; INNATE IMMUNITY ; RNA HELICASE ; TELEOST FISH ; HOST-DEFENSE
语种: 英语
WOS记录号: WOS:000352923700018
ISSN号: 1050-4648
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27115
Appears in Collections:其他_期刊论文

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作者单位: 1.Hunan Normal Univ, Coll Life Sci, Minist Educ China, Key Lab Prot Chem & Dev Biol, Changsha 410081, Hunan, Peoples R China
2.State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
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