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Identification and characterization of MAVS from black carp Mylopharyngodon piceus
Zhou, Wei1; Zhou, Jujun1; Lv, Ying1; Qu, Yixiao1; Chi, Mengdie1; Li, Jun1; Feng, Hao1,2
2015-04-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume43Issue:2Pages:460-468
AbstractMAVS (mitochondria antiviral signaling protein) plays an important role in the host cellular innate immune response against microbial pathogens. In this study, MAVS has been cloned and characterized from black carp (Mylopharyngodon piceus). The full-length cDNA of black carp MAVS (bcMAVS) consists of 2352 nucleotides and the predicted bcMAVS protein contains 579 amino acids. Structural analysis showed that bcMAVS is composed of functional domains including an N-terminal CARD, a central proline-rich domain, a putative TRAF2-binding motif and a C-terminal TM domain, which is similar to mammalian MAVS. bcMAVS is constitutively transcribed in all the selected tissues including gill, kidney, heart, intestine, liver, muscle, skin and spleen; bcMAVS mRNA level in intestine, liver, muscle increased but decreased in spleen right after GCRV or SVCV infection. Multiple bands of bcMAVS were detected in western blot when it was expressed in tissue culture, which is similar to mammalian MAVS. Immunofluorescence assay determined that bcMAVS is a mitochondria protein and luciferase reporter assay demonstrated that bcMAVS could induce zebrafish IFN and EPC IFN expression in tissue culture. Data generated in this manuscript has built a solid foundation for further elucidating the function of bcMAVS in the innate immune system of black carp. (C) 2015 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordBlack Carp Mavs Innate Immunity
DOI10.1016/j.fsi.2015.01.016
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000352923700018
WOS KeywordANTIVIRAL SIGNALING PROTEIN ; TOLL-LIKE RECEPTORS ; I-LIKE RECEPTORS ; NF-KAPPA-B ; RIG-I ; ADAPTER PROTEIN ; INNATE IMMUNITY ; RNA HELICASE ; TELEOST FISH ; HOST-DEFENSE
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27115
Collection其他_期刊论文
Affiliation1.Hunan Normal Univ, Coll Life Sci, Minist Educ China, Key Lab Prot Chem & Dev Biol, Changsha 410081, Hunan, Peoples R China
2.State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Wei,Zhou, Jujun,Lv, Ying,et al. Identification and characterization of MAVS from black carp Mylopharyngodon piceus[J]. FISH & SHELLFISH IMMUNOLOGY,2015,43(2):460-468.
APA Zhou, Wei.,Zhou, Jujun.,Lv, Ying.,Qu, Yixiao.,Chi, Mengdie.,...&Feng, Hao.(2015).Identification and characterization of MAVS from black carp Mylopharyngodon piceus.FISH & SHELLFISH IMMUNOLOGY,43(2),460-468.
MLA Zhou, Wei,et al."Identification and characterization of MAVS from black carp Mylopharyngodon piceus".FISH & SHELLFISH IMMUNOLOGY 43.2(2015):460-468.
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