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题名: Molecular mechanisms for photosynthetic carbon partitioning into storage neutral lipids in Nannochloropsis oceanica under nitrogen-depletion conditions
作者: Jia, Jing1, 2, 3; Han, Danxiang4; Gerken, Henri G.4; Li, Yantao5; Sommerfeld, Milton4; Hu, Qiang6; Xu, Jian1, 2
关键词: Nannochloropsis ; Carbon partitioning ; Storage carbohydrates ; Lipidomes ; Membrane lipids
刊名: ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
发表日期: 2015
DOI: 10.1016/j.algal.2014.11.005
卷: 7, 页:66-77
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: Polysaccharides are a major carbon/energy-reservoir in microalgae, yet their relationship with another form of carbon/energy storage, triacylglycerol (TAG), is poorly understood. Here employing oleaginous microalga Nannochloropsis oceanica as a model, we probed the crosstalk between carbohydrate metabolism and TAG accumulation by tracking the temporal dynamics of lipidomes, monosaccharides and polysaccharides and transcripts of selected genes over 14 days under nitrogen-depleted (N-) and nitrogen-replete (N+) conditions. Glucose, galactose and mannitol were the main monosaccharides in IMET1, and laminarin may be the storage polysaccharide that competes for carbon precursors with TAG. Transcriptional expression analysis revealed that the beta-1,3-glucan degradation and pyruvate dehydrogenases pathways were the main regulatory components involved in driving carbon flow to TAG synthesis. Furthermore, temporal changes of lipidomes and transcripts of glycerolipid metabolism genes were indicative of possible conversion of membrane lipids to TAG, especially under an early stage of nitrogen deprivation conditions. A carbon partitioning model for Nannochloropsis oceanica was proposed, in which beta-1,3-glucan metabolism, acetyl-CoA synthesis and membrane lipid turnover/degradation, in addition to de novo fatty acid synthesis, all contributed to TAG synthesis. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: CHLAMYDOMONAS-REINHARDTII ; DIACYLGLYCEROL ACYLTRANSFERASE ; TRIACYLGLYCEROL ACCUMULATION ; STARCH METABOLISM ; NITZSCHIA-LAEVIS ; GENOME SEQUENCE ; FATTY-ACIDS ; BIOSYNTHESIS ; MICROALGAE ; EUSTIGMATOPHYCEAE
语种: 英语
WOS记录号: WOS:000352274300010
ISSN号: 2211-9264
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27114
Appears in Collections:藻类生物技术和生物能源研发中心_期刊论文

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作者单位: 1.Chinese Acad Sci, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China
2.Chinese Acad Sci, Shandong Key Lab Energy Genet, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.AZ State Univ, Dept Appl Biol Sci, Lab Algae Res & Biotechnol, Mesa, AZ 85212 USA
5.Univ Maryland, Inst Marine & Environm Technol, Baltimore, MD 21202 USA
6.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan 430072, Hubei, Peoples R China
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