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Molecular mechanisms for photosynthetic carbon partitioning into storage neutral lipids in Nannochloropsis oceanica under nitrogen-depletion conditions
Jia, Jing1,2,3; Han, Danxiang4; Gerken, Henri G.4; Li, Yantao5; Sommerfeld, Milton4; Hu, Qiang6; Xu, Jian1,2
2015
Source PublicationALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN2211-9264
Volume7Pages:66-77
AbstractPolysaccharides are a major carbon/energy-reservoir in microalgae, yet their relationship with another form of carbon/energy storage, triacylglycerol (TAG), is poorly understood. Here employing oleaginous microalga Nannochloropsis oceanica as a model, we probed the crosstalk between carbohydrate metabolism and TAG accumulation by tracking the temporal dynamics of lipidomes, monosaccharides and polysaccharides and transcripts of selected genes over 14 days under nitrogen-depleted (N-) and nitrogen-replete (N+) conditions. Glucose, galactose and mannitol were the main monosaccharides in IMET1, and laminarin may be the storage polysaccharide that competes for carbon precursors with TAG. Transcriptional expression analysis revealed that the beta-1,3-glucan degradation and pyruvate dehydrogenases pathways were the main regulatory components involved in driving carbon flow to TAG synthesis. Furthermore, temporal changes of lipidomes and transcripts of glycerolipid metabolism genes were indicative of possible conversion of membrane lipids to TAG, especially under an early stage of nitrogen deprivation conditions. A carbon partitioning model for Nannochloropsis oceanica was proposed, in which beta-1,3-glucan metabolism, acetyl-CoA synthesis and membrane lipid turnover/degradation, in addition to de novo fatty acid synthesis, all contributed to TAG synthesis. (C) 2014 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordNannochloropsis Carbon Partitioning Storage Carbohydrates Lipidomes Membrane Lipids
DOI10.1016/j.algal.2014.11.005
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS KeywordCHLAMYDOMONAS-REINHARDTII ; DIACYLGLYCEROL ACYLTRANSFERASE ; TRIACYLGLYCEROL ACCUMULATION ; STARCH METABOLISM ; NITZSCHIA-LAEVIS ; GENOME SEQUENCE ; FATTY-ACIDS ; BIOSYNTHESIS ; MICROALGAE ; EUSTIGMATOPHYCEAE
WOS IDWOS:000352274300010
Citation statistics
Cited Times:74[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27114
Collection藻类生物技术和生物能源研发中心
Affiliation1.Chinese Acad Sci, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China
2.Chinese Acad Sci, Shandong Key Lab Energy Genet, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.AZ State Univ, Dept Appl Biol Sci, Lab Algae Res & Biotechnol, Mesa, AZ 85212 USA
5.Univ Maryland, Inst Marine & Environm Technol, Baltimore, MD 21202 USA
6.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Jia, Jing,Han, Danxiang,Gerken, Henri G.,et al. Molecular mechanisms for photosynthetic carbon partitioning into storage neutral lipids in Nannochloropsis oceanica under nitrogen-depletion conditions[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2015,7:66-77.
APA Jia, Jing.,Han, Danxiang.,Gerken, Henri G..,Li, Yantao.,Sommerfeld, Milton.,...&Xu, Jian.(2015).Molecular mechanisms for photosynthetic carbon partitioning into storage neutral lipids in Nannochloropsis oceanica under nitrogen-depletion conditions.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,7,66-77.
MLA Jia, Jing,et al."Molecular mechanisms for photosynthetic carbon partitioning into storage neutral lipids in Nannochloropsis oceanica under nitrogen-depletion conditions".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 7(2015):66-77.
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