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Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation
Li, Wen-Hua1,2; Zhou, Li1; Wang, Yang1; Shi, Jian-Tao1; Yang, Yan-Jing1,2; Gui, Jian-Fang1
2015
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
ISSN1449-2288
Volume11Issue:6Pages:688-700
Abstract

The homeobox transcription factor orthodenticle homolog 2 (otx2) is supposed as an organizer that orchestrates a transcription factor network during photoreceptor development. However, its regulation in the process remains unclear. In this study, we have identified a zebrafish limb bud and heart-like gene (lbh-like), which is expressed initially at 30 hours post fertilization (hpf) in the developing brain and eyes. Lbh-like knockdown by morpholinos specifically inhibits expression of multiple photoreceptor-specific genes, such as opsins, gnat1, gnat2 and irbp. Interestingly, otx2 expression in the morphants is not significantly reduced until 32 hpf when lbh-like begins to express, but its expression level in 72 hpf morphants is higher than that in wild type embryos. Co-injection of otx2 and its downstream target neuroD mRNAs can rescue the faults in eyes of Lbh-like morphants. Combined with the results of promoter-reporter assay, we suggest that lbh-like is a new regulator of photoreceptor differentiation directly through affecting otx2 expression in zebrafish. Furthermore, knockdown of lbh-like increases the activity of Notch pathway and perturbs the balance among proliferation, differentiation and survival of photoreceptor precursors.

SubtypeArticle
KeywordLbh-like Retina Development Photoreceptor Differentiation Otx2 Notch
DOI10.7150/ijbs.11244
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS SubjectBiochemistry & Molecular Biology ; Biology
WOS IDWOS:000359007300008
WOS KeywordTRANSCRIPTIONAL REGULATOR LBH ; HOMEOBOX GENE OTX2 ; RETINAL STEM-CELLS ; SIGNALING PATHWAY ; PROMOTES RETINOGENESIS ; EMBRYONIC-DEVELOPMENT ; NEURONAL PROGENITORS ; MAMMALIAN RETINA ; COFACTOR LBH ; MULLER GLIA
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/25976
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Univ, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Li, Wen-Hua,Zhou, Li,Wang, Yang,et al. Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2015,11(6):688-700.
APA Li, Wen-Hua,Zhou, Li,Wang, Yang,Shi, Jian-Tao,Yang, Yan-Jing,&Gui, Jian-Fang.(2015).Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,11(6),688-700.
MLA Li, Wen-Hua,et al."Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 11.6(2015):688-700.
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