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题名: Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation
作者: Li, Wen-Hua1, 2; Zhou, Li1; Wang, Yang1; Shi, Jian-Tao1; Yang, Yan-Jing1, 2; Gui, Jian-Fang1
关键词: Lbh-like ; retina development ; photoreceptor differentiation ; otx2 ; Notch
刊名: INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
发表日期: 2015
DOI: 10.7150/ijbs.11244
卷: 11, 期:6, 页:688-700
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
英文摘要: The homeobox transcription factor orthodenticle homolog 2 (otx2) is supposed as an organizer that orchestrates a transcription factor network during photoreceptor development. However, its regulation in the process remains unclear. In this study, we have identified a zebrafish limb bud and heart-like gene (lbh-like), which is expressed initially at 30 hours post fertilization (hpf) in the developing brain and eyes. Lbh-like knockdown by morpholinos specifically inhibits expression of multiple photoreceptor-specific genes, such as opsins, gnat1, gnat2 and irbp. Interestingly, otx2 expression in the morphants is not significantly reduced until 32 hpf when lbh-like begins to express, but its expression level in 72 hpf morphants is higher than that in wild type embryos. Co-injection of otx2 and its downstream target neuroD mRNAs can rescue the faults in eyes of Lbh-like morphants. Combined with the results of promoter-reporter assay, we suggest that lbh-like is a new regulator of photoreceptor differentiation directly through affecting otx2 expression in zebrafish. Furthermore, knockdown of lbh-like increases the activity of Notch pathway and perturbs the balance among proliferation, differentiation and survival of photoreceptor precursors.
关键词[WOS]: TRANSCRIPTIONAL REGULATOR LBH ; HOMEOBOX GENE OTX2 ; RETINAL STEM-CELLS ; SIGNALING PATHWAY ; PROMOTES RETINOGENESIS ; EMBRYONIC-DEVELOPMENT ; NEURONAL PROGENITORS ; MAMMALIAN RETINA ; COFACTOR LBH ; MULLER GLIA
语种: 英语
WOS记录号: WOS:000359007300008
ISSN号: 1449-2288
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/25976
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Univ, Wuhan 430072, Hubei, Peoples R China
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