Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation | |
Li, Wen-Hua1,2; Zhou, Li1; Wang, Yang1; Shi, Jian-Tao1; Yang, Yan-Jing1,2; Gui, Jian-Fang1 | |
2015 | |
Source Publication | INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
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ISSN | 1449-2288 |
Volume | 11Issue:6Pages:688-700 |
Abstract | The homeobox transcription factor orthodenticle homolog 2 (otx2) is supposed as an organizer that orchestrates a transcription factor network during photoreceptor development. However, its regulation in the process remains unclear. In this study, we have identified a zebrafish limb bud and heart-like gene (lbh-like), which is expressed initially at 30 hours post fertilization (hpf) in the developing brain and eyes. Lbh-like knockdown by morpholinos specifically inhibits expression of multiple photoreceptor-specific genes, such as opsins, gnat1, gnat2 and irbp. Interestingly, otx2 expression in the morphants is not significantly reduced until 32 hpf when lbh-like begins to express, but its expression level in 72 hpf morphants is higher than that in wild type embryos. Co-injection of otx2 and its downstream target neuroD mRNAs can rescue the faults in eyes of Lbh-like morphants. Combined with the results of promoter-reporter assay, we suggest that lbh-like is a new regulator of photoreceptor differentiation directly through affecting otx2 expression in zebrafish. Furthermore, knockdown of lbh-like increases the activity of Notch pathway and perturbs the balance among proliferation, differentiation and survival of photoreceptor precursors. |
Subtype | Article |
Keyword | Lbh-like Retina Development Photoreceptor Differentiation Otx2 Notch |
DOI | 10.7150/ijbs.11244 |
WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
Indexed By | SCI |
Language | 英语 |
WOS Research Area | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics |
WOS Subject | Biochemistry & Molecular Biology ; Biology |
WOS ID | WOS:000359007300008 |
WOS Keyword | TRANSCRIPTIONAL REGULATOR LBH ; HOMEOBOX GENE OTX2 ; RETINAL STEM-CELLS ; SIGNALING PATHWAY ; PROMOTES RETINOGENESIS ; EMBRYONIC-DEVELOPMENT ; NEURONAL PROGENITORS ; MAMMALIAN RETINA ; COFACTOR LBH ; MULLER GLIA |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/342005/25976 |
Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Univ, Wuhan 430072, Hubei, Peoples R China |
Recommended Citation GB/T 7714 | Li, Wen-Hua,Zhou, Li,Wang, Yang,et al. Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2015,11(6):688-700. |
APA | Li, Wen-Hua,Zhou, Li,Wang, Yang,Shi, Jian-Tao,Yang, Yan-Jing,&Gui, Jian-Fang.(2015).Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,11(6),688-700. |
MLA | Li, Wen-Hua,et al."Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 11.6(2015):688-700. |
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v11p0688.pdf(2628KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text |
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