IHB OpenIR  > 淡水生态学研究中心
Involvement of oxidative stress and cytoskeletal disruption in microcystin-induced apoptosis in CIK cells
Huang, Xiao1; Chen, Liang2,3; Liu, Wanjing1; Qiao, Qin1; Wu, Kang1; Wen, Jing1; Huang, Cuihong1; Tang, Rong1; Zhang, Xuezhen1
2015-08-01
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume165Pages:41-50
AbstractThe outbreak of cyanobacterial blooms induces the production and release of microcystins (MCs) into water, representing a health hazard to aquatic organisms and even humans. Some recent studies have suggested that kidney is another important target organ of MCs except liver, however, the potential toxicity mechanisms are still unclear. In this study, we first investigated the collaborative effect of oxidative stress and cytoskeletal disruption in microcystin-induced apoptosis in CIK (Ctenopharyngodon idellus kidney) cells in vitro. CIK cells were treated with 0, 1, 10, and 100 mu g/L microcystin-LR (MC-LR) for 24 and 48 h. Cell viability was increased by MC-LR in 1 mu g/L group, while decreased in 100 mu g/L group at 48 h. Cell cycle assay showed that 1 and 10 mu g/L MC-LR induced cell cycle through G(1) into S and G(2)/M phases, while 100 mu g/L MC-LR reduced G2/M phase population. MC-LR markedly induced apoptosis in 10 and 100 mu g/L groups. Elevated reactive oxygen species (ROS) production, increased malondialdehyde (MDA) contents, decreased glutathione (GSH) levels, and modulated antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) were observed in CIK cells exposed to MC-LR. These alterations were more pronounced at higher doses (10 and 100 mu g/L), indicating that oxidative stress was induced by MC-LR. Laser scanning confocal microscope observation showed aggregation and collapse of microfilaments (MFs) and microtubules (MTs) in CIK cells, and even loss of some cytoskeleton structure. Moreover, transcriptional changes of cytoskeletal genes (beta-actin, Ic3a, and keratin) were also determined, which have a high probability with cytoskeleton structure damage. Our data suggest that oxidative stress and cytoskeletal disruption may interact with each other and jointly lead to apoptosis and renal toxicity induced by MCs. (C) 2015 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordMicrocystin-lr Apoptosis Oxidative Stress Cytoskeleton Cik Cell Nephrotoxicity
DOI10.1016/j.aquatox.2015.05.009
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; OXYGEN SPECIES FORMATION ; PRIMARY LIVER-CANCER ; IN-VITRO ; CULTURED-HEPATOCYTES ; ACTIN CYTOSKELETON ; RAT HEPATOCYTES ; REFERENCE GENES ; DRINKING-WATER ; LR
WOS IDWOS:000359030300005
Citation statistics
Cited Times:29[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/25975
Collection淡水生态学研究中心
Affiliation1.Huazhong Agr Univ, Coll Fisheries, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
3.Univ Chinese Acad Sci, Being 100049, Peoples R China
Recommended Citation
GB/T 7714
Huang, Xiao,Chen, Liang,Liu, Wanjing,et al. Involvement of oxidative stress and cytoskeletal disruption in microcystin-induced apoptosis in CIK cells[J]. AQUATIC TOXICOLOGY,2015,165:41-50.
APA Huang, Xiao.,Chen, Liang.,Liu, Wanjing.,Qiao, Qin.,Wu, Kang.,...&Zhang, Xuezhen.(2015).Involvement of oxidative stress and cytoskeletal disruption in microcystin-induced apoptosis in CIK cells.AQUATIC TOXICOLOGY,165,41-50.
MLA Huang, Xiao,et al."Involvement of oxidative stress and cytoskeletal disruption in microcystin-induced apoptosis in CIK cells".AQUATIC TOXICOLOGY 165(2015):41-50.
Files in This Item:
File Name/Size DocType Version Access License
Involvement of oxida(2759KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Huang, Xiao]'s Articles
[Chen, Liang]'s Articles
[Liu, Wanjing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Huang, Xiao]'s Articles
[Chen, Liang]'s Articles
[Liu, Wanjing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Huang, Xiao]'s Articles
[Chen, Liang]'s Articles
[Liu, Wanjing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Involvement of oxidative stress and cytoskeletal disruption in microcystin-induced apoptosis in CIK cells.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.