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题名: Involvement of oxidative stress and cytoskeletal disruption in microcystin-induced apoptosis in CIK cells
作者: Huang, Xiao1; Chen, Liang2, 3; Liu, Wanjing1; Qiao, Qin1; Wu, Kang1; Wen, Jing1; Huang, Cuihong1; Tang, Rong1; Zhang, Xuezhen1
关键词: Microcystin-LR ; Apoptosis ; Oxidative stress ; Cytoskeleton ; CIK cell ; Nephrotoxicity
刊名: AQUATIC TOXICOLOGY
发表日期: 2015-08-01
DOI: 10.1016/j.aquatox.2015.05.009
卷: 165, 页:41-50
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Marine & Freshwater Biology ; Toxicology
研究领域[WOS]: Marine & Freshwater Biology ; Toxicology
英文摘要: The outbreak of cyanobacterial blooms induces the production and release of microcystins (MCs) into water, representing a health hazard to aquatic organisms and even humans. Some recent studies have suggested that kidney is another important target organ of MCs except liver, however, the potential toxicity mechanisms are still unclear. In this study, we first investigated the collaborative effect of oxidative stress and cytoskeletal disruption in microcystin-induced apoptosis in CIK (Ctenopharyngodon idellus kidney) cells in vitro. CIK cells were treated with 0, 1, 10, and 100 mu g/L microcystin-LR (MC-LR) for 24 and 48 h. Cell viability was increased by MC-LR in 1 mu g/L group, while decreased in 100 mu g/L group at 48 h. Cell cycle assay showed that 1 and 10 mu g/L MC-LR induced cell cycle through G(1) into S and G(2)/M phases, while 100 mu g/L MC-LR reduced G2/M phase population. MC-LR markedly induced apoptosis in 10 and 100 mu g/L groups. Elevated reactive oxygen species (ROS) production, increased malondialdehyde (MDA) contents, decreased glutathione (GSH) levels, and modulated antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) were observed in CIK cells exposed to MC-LR. These alterations were more pronounced at higher doses (10 and 100 mu g/L), indicating that oxidative stress was induced by MC-LR. Laser scanning confocal microscope observation showed aggregation and collapse of microfilaments (MFs) and microtubules (MTs) in CIK cells, and even loss of some cytoskeleton structure. Moreover, transcriptional changes of cytoskeletal genes (beta-actin, Ic3a, and keratin) were also determined, which have a high probability with cytoskeleton structure damage. Our data suggest that oxidative stress and cytoskeletal disruption may interact with each other and jointly lead to apoptosis and renal toxicity induced by MCs. (C) 2015 Elsevier B.V. All rights reserved.
关键词[WOS]: TROUT ONCORHYNCHUS-MYKISS ; OXYGEN SPECIES FORMATION ; PRIMARY LIVER-CANCER ; IN-VITRO ; CULTURED-HEPATOCYTES ; ACTIN CYTOSKELETON ; RAT HEPATOCYTES ; REFERENCE GENES ; DRINKING-WATER ; LR
语种: 英语
WOS记录号: WOS:000359030300005
ISSN号: 0166-445X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/25975
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Huazhong Agr Univ, Coll Fisheries, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
3.Univ Chinese Acad Sci, Being 100049, Peoples R China
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