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Analysis of the nicotinamide phosphoribosyltransferase family provides insight into vertebrate adaptation to different oxygen levels during the water-to-land transition
Fang, Chengchi1,2; Guan, Lihong1,2; Zhong, Zaixuan1,2; Gan, Xiaoni1; He, Shunping1
2015-08-01
Source PublicationFEBS JOURNAL
ISSN1742-464X
Volume282Issue:15Pages:2858-2878
AbstractOne of the most important events in vertebrate evolutionary history is the water-to-land transition, during which some morphological and physiological changes occurred in concert with the loss of specific genes in tetrapods. However, the molecular mechanisms underlying this transition have not been well explored. To explore vertebrate adaptation to different oxygen levels during the water-to-land transition, we performed comprehensive bioinformatics and experimental analysis aiming to investigate the NAMPT family in vertebrates. NAMPT, a rate-limiting enzyme in the salvage pathway of NAD+ biosynthesis, is critical for cell survival in a hypoxic environment, and a high level of NAMPT significantly augments oxidative stress in normoxic environments. Phylogenetic analysis showed that NAMPT duplicates arose from a second round whole-genome duplication event. NAMPTA existed in all classes of vertebrates, whereas NAMPTB was only found in fishes and not tetrapods. Asymmetric evolutionary rates and purifying selection were the main evolutionary forces involved. Although functional analysis identified several functionally divergent sites during NAMPT family evolution, invitro experimental data demonstrated that NAMPTA and NAMPTB were functionally conserved for NAMPT enzymatic function in the NAD+ salvage pathway. Insitu hybridization revealed broad NAMPTA and NAMPTB expression patterns, implying regulatory functions over a wide range of developmental processes. The morpholino-mediated knockdown data demonstrated that NAMPTA was more essential than NAMPTB for vertebrate embryo development. We propose that the retention of NAMPTB in water-breathing fishes and its loss in air-breathing tetrapods resulted from vertebrate adaptation to different oxygen levels during the water-to-land transition.
SubtypeArticle
KeywordAdaptation Nampt Oxygen Level Vertebrates Water-to-land Transition
DOI10.1111/febs.13327
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS KeywordCOLONY-ENHANCING FACTOR ; FUNCTIONAL DIVERGENCE ; MAXIMUM-LIKELIHOOD ; BIOSYNTHETIC ENZYME ; POSITIVE SELECTION ; GENE DUPLICATION ; CELL-SURVIVAL ; EVOLUTION ; VISFATIN ; EXPRESSION
WOS IDWOS:000358840500006
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/25973
Collection水生生物多样性与资源保护研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Fang, Chengchi,Guan, Lihong,Zhong, Zaixuan,et al. Analysis of the nicotinamide phosphoribosyltransferase family provides insight into vertebrate adaptation to different oxygen levels during the water-to-land transition[J]. FEBS JOURNAL,2015,282(15):2858-2878.
APA Fang, Chengchi,Guan, Lihong,Zhong, Zaixuan,Gan, Xiaoni,&He, Shunping.(2015).Analysis of the nicotinamide phosphoribosyltransferase family provides insight into vertebrate adaptation to different oxygen levels during the water-to-land transition.FEBS JOURNAL,282(15),2858-2878.
MLA Fang, Chengchi,et al."Analysis of the nicotinamide phosphoribosyltransferase family provides insight into vertebrate adaptation to different oxygen levels during the water-to-land transition".FEBS JOURNAL 282.15(2015):2858-2878.
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