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题名: Functions of the two zebrafish MAVS variants are opposite in the induction of IFN1 by targeting IRF7
作者: Lu, Long-Feng1, 2; Li, Shun1; Lu, Xiao-Bing1, 2; Zhang, Yong-An1
关键词: MAVS variants ; Negative regulator ; Interferon ; IRF7 ; Zebrafish
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2015-08-01
DOI: 10.1016/j.fsi.2015.05.019
卷: 45, 期:2, 页:574-582
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
英文摘要: IFNs create the first line of host cells to defense viral infection, however, unrestricted expression of IFN can be hazardous to the host. IRF7 is the master regulator of type I IFN expression. To our knowledge, non research about the inhibition of IFN expression by targeting IRF7 has been reported in fish. In this study, we reported that the splicing variant of wildtype MAVS (MAVS_tv1), MAVS_tv2, negatively regulated IRF7-mediated IFN production. Firstly, in vivo, the transcriptional levels of MAVS_tv2 in trunk kidney and spleen from the zebrafish infected with SVCV were monitored. Then, in vitro, the protein expression pattern of MAVS_tv2 in zebrafish cell lines was detected using anti-MAVS_tv2 antibody. Furthermore, overexpression of MAVS_tv2 decreased the activation of IFNI promoter that induced by IRF7 in a dose-dependent manner, whereas it had little effect on IRF3, a close relative of IRF7. In addition, such inhibition was also observed in IRF7-mediated epcIFN promoter and ISRE activities, but not in the activation of the promoters of type II IFNs and NF-kappa B, due to IRF7 not regulating their expression. Lastly, overexpression of MAVS_tv2 decreased the transcriptional levels of several IFN-stimulated genes activated by IRF7. These findings suggest that MAVS_tv2 is a negative regulator of IFNI by targeting IRF7. (C) 2015 Elsevier Ltd. All rights reserved.
关键词[WOS]: ANTIVIRAL SIGNALING PROTEIN ; INNATE IMMUNE-RESPONSE ; TOLL-LIKE RECEPTORS ; HEPATITIS-C-VIRUS ; NF-KAPPA-B ; RIG-I ; PATHOGEN RECOGNITION ; NEGATIVE REGULATION ; SPLICING VARIANTS ; ADAPTER PROTEIN
语种: 英语
WOS记录号: WOS:000358626800043
ISSN号: 1050-4648
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/25972
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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