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Functions of the two zebrafish MAVS variants are opposite in the induction of IFN1 by targeting IRF7
Lu, Long-Feng1,2; Li, Shun1; Lu, Xiao-Bing1,2; Zhang, Yong-An1
2015-08-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume45Issue:2Pages:574-582
AbstractIFNs create the first line of host cells to defense viral infection, however, unrestricted expression of IFN can be hazardous to the host. IRF7 is the master regulator of type I IFN expression. To our knowledge, non research about the inhibition of IFN expression by targeting IRF7 has been reported in fish. In this study, we reported that the splicing variant of wildtype MAVS (MAVS_tv1), MAVS_tv2, negatively regulated IRF7-mediated IFN production. Firstly, in vivo, the transcriptional levels of MAVS_tv2 in trunk kidney and spleen from the zebrafish infected with SVCV were monitored. Then, in vitro, the protein expression pattern of MAVS_tv2 in zebrafish cell lines was detected using anti-MAVS_tv2 antibody. Furthermore, overexpression of MAVS_tv2 decreased the activation of IFNI promoter that induced by IRF7 in a dose-dependent manner, whereas it had little effect on IRF3, a close relative of IRF7. In addition, such inhibition was also observed in IRF7-mediated epcIFN promoter and ISRE activities, but not in the activation of the promoters of type II IFNs and NF-kappa B, due to IRF7 not regulating their expression. Lastly, overexpression of MAVS_tv2 decreased the transcriptional levels of several IFN-stimulated genes activated by IRF7. These findings suggest that MAVS_tv2 is a negative regulator of IFNI by targeting IRF7. (C) 2015 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordMavs Variants Negative Regulator Interferon Irf7 Zebrafish
DOI10.1016/j.fsi.2015.05.019
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000358626800043
WOS KeywordANTIVIRAL SIGNALING PROTEIN ; INNATE IMMUNE-RESPONSE ; TOLL-LIKE RECEPTORS ; HEPATITIS-C-VIRUS ; NF-KAPPA-B ; RIG-I ; PATHOGEN RECOGNITION ; NEGATIVE REGULATION ; SPLICING VARIANTS ; ADAPTER PROTEIN
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Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/25972
Collection水生生物分子与细胞生物学研究中心_期刊论文
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Lu, Long-Feng,Li, Shun,Lu, Xiao-Bing,et al. Functions of the two zebrafish MAVS variants are opposite in the induction of IFN1 by targeting IRF7[J]. FISH & SHELLFISH IMMUNOLOGY,2015,45(2):574-582.
APA Lu, Long-Feng,Li, Shun,Lu, Xiao-Bing,&Zhang, Yong-An.(2015).Functions of the two zebrafish MAVS variants are opposite in the induction of IFN1 by targeting IRF7.FISH & SHELLFISH IMMUNOLOGY,45(2),574-582.
MLA Lu, Long-Feng,et al."Functions of the two zebrafish MAVS variants are opposite in the induction of IFN1 by targeting IRF7".FISH & SHELLFISH IMMUNOLOGY 45.2(2015):574-582.
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