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Effects of microcystin-LR on gut microflora in different gut regions of mice
Chen, Jing1,2; Xie, Ping1; Lin, Juan1,2; He, Jun1; Zeng, Cheng3; Chen, Jun1
2015-08-01
Source PublicationJOURNAL OF TOXICOLOGICAL SCIENCES
ISSN0388-1350
Volume40Issue:4Pages:485-494
AbstractTo reveal the toxicological effects of the hepatotoxic microcystin-leucine arginine (MC-LR) on gut microbial community composition in different gut regions, we conducted a subchronic exposure of BALB/c mice to MC-LR via intragastric administration. Denaturing gradient gel electrophoresis (DGGE) was employed to profile the shifts of microbes after MC-LR treatment in the jejuno-ileum, caecum and colon. DGGE profiles analysis showed that MC-LR increased the microbial species richness (number of microbial bands) in the caecum and colon as well as microbial diversity (Shannon-Wiener index) in the caecum. The cluster analysis of DGGE profiles indicated that the microbial structures in the caecum and colon shifted significantly after MC-LR treatment, while that in the jejuno-ileum did not. All the relatively decreased gut microbes belonged to Clostridia in the Firmicutes phylum, and most of them were Lachnospiraceae. The increased ones derived from a variety of microbes including species from Porphyromonadaceae and Prevotellaceae in the Bacteroidetes phylum, as well as Lachnospiraceae and Ruminococcaceae in the Firmicutes phylum, and among which, the increase of Barnesiella in Porphyromonadaceae was most remarkable. In conclusion, subchronic exposure to MC-LR could disturb the balance of gut microbes in mice, and its toxicological effects varied between the jejuno-ileum and the other two gut regions.
SubtypeArticle
KeywordMc-lr Gut Microbial Community Mice Intestine
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaToxicology
WOS SubjectToxicology
WOS KeywordSPINNING H-1-NMR SPECTROSCOPY ; FATTY LIVER-DISEASE ; IN-VITRO EVALUATION ; 16S RIBOSOMAL-RNA ; BACTERIAL DIVERSITY ; INTESTINAL MICROBIOTA ; CYANOBACTERIAL TOXINS ; MICROORGANISMS ; HEALTH ; GENES
WOS IDWOS:000359180600007
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/25971
Collection淡水生态学研究中心
Affiliation1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Chen, Jing,Xie, Ping,Lin, Juan,et al. Effects of microcystin-LR on gut microflora in different gut regions of mice[J]. JOURNAL OF TOXICOLOGICAL SCIENCES,2015,40(4):485-494.
APA Chen, Jing,Xie, Ping,Lin, Juan,He, Jun,Zeng, Cheng,&Chen, Jun.(2015).Effects of microcystin-LR on gut microflora in different gut regions of mice.JOURNAL OF TOXICOLOGICAL SCIENCES,40(4),485-494.
MLA Chen, Jing,et al."Effects of microcystin-LR on gut microflora in different gut regions of mice".JOURNAL OF TOXICOLOGICAL SCIENCES 40.4(2015):485-494.
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