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Comparative Transcriptome Analysis of Differentially Expressed Genes and Signaling Pathways between XY and YY Testis in Yellow Catfish
Wu, Junjie1; Xiong, Shuting1; Jing, Jing1; Chen, Xin1; Wang, Weimin1; Gui, Jian-Fang1,2; Mei, Jie1
2015-08-04
Source PublicationPLOS ONE
ISSN1932-6203
Volume10Issue:8
AbstractYY super-males have rarely been detected in nature and only been artificially created in some fish species including tilapia and yellow catfish (Pelteobagrusfulvidraco), which provides a promising model for testis development and spermatogenesis. In our previous study, significant differences in morphology and miRNA expression were detected between XY and YY testis of yellow catfish. Here, solexa sequencing technology was further performed to compare mRNA expression between XY and YY testis. Compared with unigenes expressed in XY testis, 1146 and 1235 unigenes have significantly higher and lower expression in YY testis, respectively. 605 differentially expressed unigenes were annotated to 1604 GO terms with 319 and 286 genes having relative higher expression in XY and YY testis. KEGG analysis suggested different levels of PI3K-AKT and G protein-coupled receptor (GPCR) signaling pathways between XY and YY testis. Down-regulation of miR-141/429 in YY testis was speculated to promote testis development and maturation, and several factors in PI3K-AKT and GPCR signaling pathways were found as predicted targets of miR-141/429, several of which were confirmed by dual-luciferase reporter assays. Our study provides a comparative transcriptome analysis between XY and YY testis, and reveals interactions between miRNAs and their target genes that are possibly involved in regulating testis development and spermatogenesis.
SubtypeArticle
DOI10.1371/journal.pone.0134626
WOS HeadingsScience & Technology
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS KeywordSEX-DETERMINING REGION ; GERM-CELL DEVELOPMENT ; TYROSINE PHOSPHORYLATION ; ONCORHYNCHUS-MYKISS ; MICRORNA TARGETS ; BOAR SPERMATOZOA ; RAINBOW-TROUT ; FISH ; MARKERS ; ACTIVATION
WOS IDWOS:000358942700054
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/25970
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Key Lab Freshwater Anim Breeding, Coll Fisheries,Minist Agr, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Wu, Junjie,Xiong, Shuting,Jing, Jing,et al. Comparative Transcriptome Analysis of Differentially Expressed Genes and Signaling Pathways between XY and YY Testis in Yellow Catfish[J]. PLOS ONE,2015,10(8).
APA Wu, Junjie.,Xiong, Shuting.,Jing, Jing.,Chen, Xin.,Wang, Weimin.,...&Mei, Jie.(2015).Comparative Transcriptome Analysis of Differentially Expressed Genes and Signaling Pathways between XY and YY Testis in Yellow Catfish.PLOS ONE,10(8).
MLA Wu, Junjie,et al."Comparative Transcriptome Analysis of Differentially Expressed Genes and Signaling Pathways between XY and YY Testis in Yellow Catfish".PLOS ONE 10.8(2015).
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