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题名: Dietary phenylalanine-improved intestinal barrier health in young grass carp (Ctenopharyngodon idella) is associated with increased immune status and regulated gene expression of cytokines, tight junction proteins, antioxidant enzymes and related signalling molecules
作者: Feng, Lin1, 2, 3; Li, Wen1; Liu, Yang1, 2, 3; Jiang, Wei-Dan1, 2, 3; Kuang, Sheng-Yao4; Jiang, Jun1, 2, 3; Tang, Ling4; Wu, Pei1, 2, 3; Tang, Wu-Neng4; Zhang, Yong-An5; Zhou, Xiao-Qiu1, 2, 3
关键词: Phenylalanine ; Immune status ; Cytokines ; Tight junction proteins ; TOR ; Nrf
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2015-08-01
DOI: 10.1016/j.fsi.2015.05.001
卷: 45, 期:2, 页:495-509
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
英文摘要: The present work evaluated the effects of dietary phenylalanine (Phe) on the intestinal immune response, tight junction proteins transcript abundance, and the gene expression of immune- and antioxidant-related signalling molecules in the intestine. In addition, the dietary Phe (and Phe + Tyr) requirement of young grass carp (Ctenopharyngodon idella) was also estimated. Fish were fed fish meal -casein-gelatin based diets (302.3 g crude protein kg(-1)) containing 3.4 (basal diet), 6.1, 9.1, 11.5, 14.0 and 16.8 g Phe kg(-1), with a fixed amount of 10.7 g tyrosine kg(-1) for 8 weeks. The results showed that Phe deficiency or excess Phe reduced the lysozyme and acid phosphatase activities and complement C 3 content in the intestine (P < 0.05). Moreover, zonula occludens-1 (ZO-1), ocdudin and claudin c mRNA levels were highest in the fish fed the diet containing 11.5 g Phe kg(-1) (P < 0.05). However, claudin 12 and claudin b mRNA levels were not significantly affected by dietary Phe (P > 0.05). Gene expression of interleukin-10 (IL-10), transforming growth factor-beta 1 (TGF-beta 1), target of rapamycin (TOR) and inhibitor of nuclear factor kappa B alpha (I kappa B alpha) in proximal intestine (PI), mid intestine (MI) and distal intestine (DI) increased as dietary Phe increased up to 6.1, 9.1, 11.5 and 14.0 g kg(-1), respectively (P < 0.05). However, interleukin-8 (IL-8), tumour necrosis factor-alpha (TNF-alpha) and nuclear factor-kappa B p6.5 (NF-kappa B p65) mRNA levels showed opposite tendencies. In addition, the mRNA level of superoxide dismutase (SOD) was significantly lower in the intestinal tissue of the group fed a diet with Phe levels of 16.8 g kg(-1) than in those of other groups (P < 0.05). The expression of NF-E2-related factor 2 (Nrf2) gene was increased as dietary Phe increased up to 9.1 g kg(-1) (P < 0.05). In conclusion, Phe improved intestinal immune status, and regulated gene expression of cytokines, tight junction proteins, antioxidant enzymes, NF-kappa B p65, I kappa B alpha, TOR, and Nrf2 in the fish intestine. Based on the quadratic regression analysis of lysozyme activity at a 95% maximum, the dietary Phe requirement of young grass carp (256-629 g) was estimated to be 8.31 g kg(-1), corresponding to 2.75 g 100 g(-1) protein. (C) 2015 Elsevier Ltd. All rights reserved.
关键词[WOS]: NF-KAPPA-B ; INFLAMMATORY-BOWEL-DISEASE ; AMINO-ACID REQUIREMENT ; OXIDATIVE STRESS ; EPITHELIAL PERMEABILITY ; PROINFLAMMATORY CYTOKINES ; MAMMALIAN TARGET ; INNATE IMMUNITY ; JUVENILE JIAN ; DANIO-RERIO
语种: 英语
WOS记录号: WOS:000358626800034
ISSN号: 1050-4648
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/25969
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
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